Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

Abstract

In the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experiencein vivowhile pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50–500 ng mL−1. Further, the standard sample preparation of this method can be used as a guideline for other methods, particularly when highly hydrophobic drugs with considerable protein binding are involved and could be valuable in the field of bioanalysis to improve the reliability of methods.

Comparative pharmacokinetics of four active components on normal and diabetic rats after oral administration of Gandi capsules

“…18,19 Some articles have reported about the in vivo process of baicalin, wogonoside, wogonin, and loganin respectively or in other compound preparations. [20][21][22][23] However, few analytical methods were developed for simultaneous determination of the four compounds in rat plasma and to describe the prole of Gandi capsule in vivo aer oral administration.…”