Studies on the association of<i>BF</i>1/<i>BF</i>2 gene expression patterns with traits of genetic resistance to Marek's disease in chickens

Jin, Yuanchang; Wei, Ping; Niu, Bo-Xue; Li, Meng; Yang, Qingli

doi:10.1080/09540105.2013.768963

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…The Xiayan is a well‐known and the largest local line of the meat‐type chicken in China. During about 5 years of research, we have arrived at almost the same conclusion that we present in this study . Also, in narrowing down the BF gene further to it's alternative exon 7 splice variant and especially in developing a snPCR assay that amplifies the alternative exon 7 splice variant of the BF gene , we have increased the chance for the rapid detection of resistant/susceptible BF haplotypes to MD .…”

Section: Discussionsupporting

confidence: 71%

The Relationship Between the Alternative exon 7 Splice Variant of the BF Gene and MHC‐Related Marek's Disease Resistance in Chickens

et al. 2014

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The study was to analyse the relationship between the alternative exon 7 splice variant of the BF gene and MHC-related Marek's disease (MD) resistance in chickens. The experiment first determined whether or not the cocks of Xiayan chickens have alternative splicing-out of the exon 7 of the BF gene from peripheral blood leucocytes (PBLs). Then, selected two groups: Group K included the offspring of the selected cocks which have no alternative splicingout of the exon 7 of the BF gene; Group Y included the offspring of the selected cocks which have alternative splicing-out of the exon 7 of the BF gene. All hens used in the cross-breeding were non-selected. The experimental chickens were challenged with a very virulent strain of Marek's disease virus (MDV) at 4 days old and were raised for 12 weeks. At this time, all the surviving chickens were killed and necropsy was also performed during the experiment whenever chickens died from the infection. Tumour incidence and mortality were calculated using SPSS, and the tissues were collected to detect MDV by PCR. The results showed that the mortalities of Group K and Y were 52.75% and 70.65%, respectively; and that the tumour incidences of non-alternative splicing-out of the exon 7 of the BF for Groups K and Y were 15.38% and 38.89%, respectively; the tumour incidences for the alternative splicing-out of the exon 7 were 46.15% and 56.76%, respectively. The results demonstrated the tumour incidence was highly related with the alternative exon 7 splice variant of the BF gene (P < 0.05).

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Section: Discussionsupporting

confidence: 71%

The Relationship Between the Alternative exon 7 Splice Variant of the BF Gene and MHC‐Related Marek's Disease Resistance in Chickens

et al. 2014

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“…The 20 N-terminal amino acid sequences of the 37 kDa OMP have a high homology to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which catalyzes glycolysis in eukaryotes. GAPDH is also found on the bacterial cell surface and is used as a house keeping gene and protein in quantitative polymerase chain reaction (PCR) and western blot analysis respectively (Jin, Wei, Niu, Li, & Yang, 2014;Ueno, Nishiguchi, Takeshita, Yamaguchi, & Oda, 2017;Wu et al, 2012;Yu et al, 2011;Zainuddin, Chua, Rahim, & Makpol, 2010).…”

Section: Introductionmentioning

confidence: 99%

Monoclonal antibodies against recombinant GAPDH of Edwardsiella tarda reveal the conserved nature of the protein

Banu

Anand

Bedekar

et al. 2017

Food and Agricultural Immunology

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The outer membrane protein, encoded by glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, of Edwardsiella tarda is a highly conserved immunogenic protein. The GAPDH was cloned and expressed in Escherichia coli. The purified protein was used to produce mouse monoclonal antibodies (MAbs). Four stable hybridomas producing MAbs (3G12, 4E9, 5A11 and 9G1) against rGAPDH were obtained. The heavy chains of antibodies produced by the hybridomas were of the isotypes IgG1 and IgM. Cross reactivity of MAbs (3G12 and 9G1) was observed with GAPDH of Aeromonas hydrophila and Micrococcus luteus. MAbs 3G12 and 4E9 reacted with Vibrio cholerae, Salmonella enterica and Penaeus monodon tissues but not with vertebrate GAPDH. None of the MAbs reacted with Staphylococcus aureus. The results indicate that the level of conservation of GAPDH is high among evolutionarily close species. The MAbs developed will be a useful tool to study the evolutionarily conserved and functionally diverse GAPDH. ARTICLE HISTORY

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“…BF1 was poorly transcribed and BF2 well transcribed at a ratio varying from 1.5 to 33, supporting the 'minimal essential MHC' hypothesis that a single dominant class I molecule (BF2) determines MHC haplotype-associated differences in disease resistance. Previously, Wei [1] found that the expression modes of BF1 and BF2 genes might be related to traits of genetic resistance to MD in the chicken. However, variation in amino acid sequences between the full-length BF1 and BF2 mRNA transcripts has not been compared.…”

mentioning

confidence: 99%

Comparison of Full‐length Amino Acid Sequences of Chickens BF1 and BF2

et al. 2015

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Studies on the association ofBF1/BF2 gene expression patterns with traits of genetic resistance to Marek's disease in chickens

Cited by 3 publications

References 21 publications

The Relationship Between the Alternative exon 7 Splice Variant of the BF Gene and MHC‐Related Marek's Disease Resistance in Chickens

The Relationship Between the Alternative exon 7 Splice Variant of the BF Gene and MHC‐Related Marek's Disease Resistance in Chickens

Monoclonal antibodies against recombinant GAPDH of Edwardsiella tarda reveal the conserved nature of the protein

Comparison of Full‐length Amino Acid Sequences of Chickens BF1 and BF2

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