STUDIES ON THE ANTIGENIC STRUCTURE OF THE 80/81 COMPLEX OF <i>STAPHYLOCOCCUS AUREUS</i>

Hofstad, Tor

doi:10.1111/apm.1965.65.1.134

Cited by 8 publications

(8 citation statements)

References 21 publications

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“…Similar results were obtained by Yoshida et al (34) who demonstrated that their purified protein A removed 94% of the agglutinins in normal human sera, Lofqvist (23), using specific rabbit antiserum to protein A, was able to remove the agglutinins of S. aureus in serum by adding protein A. Similar results were obtained by Hofstad (15).…”

Section: Discussionsupporting

confidence: 84%

“…From the adsorption experiments with protein A described above (15,16,23,34), it is impossible to conclude that protein A is the substance responsible for agglutination by normal rabbit sera. It is now well known that protein A reacts with the Fc part of mammalian IgG (8.9, 18).…”

Section: Discussionmentioning

confidence: 99%

“…Agglutination of several Staphylococcus aureus strains by normal sera (man, rabbit, mouse) has been observed by many investigators (3,4,9,16). Protein A, a cell wall component of S. aureus (6,13,15,23,34) which reacts with the Fc fragment of immunoglobulin G (IgG) from different species (8,9,18,20), has been suggested to be identical with the agglutinogen (16,17,34). Other investigators have shown that protein A and the agglutinogen are not identical substances (3,4,11).…”

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confidence: 99%

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Agglutination of Staphylococcus aureus by Rabbit Sera

Forsgren¹,

Forsum²

1972

Infect Immun

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Of 137 Staphylococcus aureus strains, 87 agglutinated in normal rabbit serum. The agglutination was shown to be caused by the Fc-part of immunoglobulin G (IgG). F(ab 1 ) 2 -fragments of IgG and immunoglobulin M (IgM) in corresponding concentrations were unreactive. The agglutinating strains had a high or moderate content of protein A. Strains with a low content of protein A and protein A-negative mutants did not agglutinate. The importance of the reaction between the Fc part of IgG and protein A for serotyping of S. aureus is demonstrated. Two alternative methods for serotyping S. aureus are suggested, using either F(ab 1 ) 2 fragments of IgG or intact IgM.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Agglutination of Staphylococcus aureus by Rabbit Sera

Forsgren¹,

Forsum²

1972

Infect Immun

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“…Assays were performed on 0.1-mg quantities of antigen with 0.1 mg of enzyme in 1 ml of maleate buffer (1 mM sodium maleate, 1 mM ethylenediaminetetraacetic acid; pH 6.5) with incubation at 37 C for 24 h. After incubation the mixture was dialyzed three times against DDD water and adjusted to a final volume of 1.0 ml. Periodate treatment of the purified antigen was carried out as described by Hofstad (13). After treatment, the mixture was dialyzed three times against DDD watter and the final volume was adjusted so that the concentration of the antigen would be 100 ,gg/ml (this was based on the original weight of the extract).…”

mentioning

confidence: 99%

Cross-reactions of Streptococcus mutans due to cell wall teichoic acid

Chorpenning¹,

Cooper²,

Rosen³

1975

Infect Immun

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Antisera to the whole cells of Streptococcus mutans cross-reacted with antigen extracts from four other gram-positive species, as well as with those of three other oral streptococci. Similarly, antisera to these bacteria cross-reacted with extracts from S. mutans and with those from each other. Using a purified phenol extract of the walls of S. mutans, which was identified by chemical, immunochemical, and enzymatic analyses as glycerol teichoic acid, the cross-reactions were shown to be specific for a determinant of the teichoic acid backbone. Results were confirmed in immunodiffusion tests where clear bands of identity were shown. These observations point out the need for caution in serological research employing extracts of gram-positive bacteria and may be of interest in investigations of periodontal disease.on August 3, 2020 by guest

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“…Although biochemical criteria have recently proved useful in classifying such phage-untypable cultures with respect to human or canine host specificity (6,8), these properties, likewise, are considered subject to change (4). The antigenic makeup of staphylococci, on the other hand, has been reported to be a stable characteristic (3,5,11) so that an agglutinating serum for distinguishing S. aureus of human origin would seem advantageous. Absorption of immune serum 17 solely with S. aureus strain 61218 resulted in an agglutinating serum which appeared specific for thermolabile agglutinogen of S. aureus of human biotype.…”

mentioning

confidence: 99%