Agglutinating serum for distinguishing Staphylococcus aureus of human biotype

Abstract

Antiserum to Staphylococcus aureus strain 17 was treated with S. aureus strain 61218 until the antibodies against thermostable agglutinogen were removed. The absorbed serum agglutinated phage-typable as well as phageuntypable staphylococci of human biotype, whether recovered from people or from dogs.

New serologically distinguishable type of coagulase-positive staphylococcus of canine origin

“…The fibrinolysin test, the coagulase test with human and canine plasma, the production of immune sera, the preparation of absorbed immune serum to strain 61218, and the slide agglutination test were done as previously described (6). Absorbed immune serum to strain 17 was prepared as previously described (7). The preparation of absorbed immune serum to strain 887 was analogous to that of absorbed immune serum 61218, i.e., immune serum 887 was absorbed in succession with strains 17 and Cowan I (11).…”

Specific and cross-reacting antigens of Staphylococcus aureus of human and canine origins

“…As in serological studies in general, in this report S. aureus designates coagulase-positive staphylococci as a group by the classification in Bergey's Manual of Determinative Bacteriology (2), without biochemical differentiation of the recently proposed species Staphylococcus intermedius (3). After proper absorption, antiserum against strain 61218 and antiserum against strain 17, group-representative strains of S. aureus of canine and human origins, respectively, have been found to agglutinate specifically S. aureus of canine and human origins (5,6,11). The untreated antisera will be referred to as antiserum 61218 and antiserum 17, whereas after the necessary absorption they will be designated as absorbed antiserum 61218 and absorbed antiserum 17.…”