Studies on the anti-inflammatory effects ofclerodendron trichotomum thunberg leaves

Abstract

Clerodendron trichotomum Thunberg Leaves (CTL) have been used for centuries in Chinese folk medicine for their anti-inflammatory properties. We have studied the anti-inflammatory effects of CTL extracts in rats, mice and in Raw 264.7 cells. 1 mg/kg solutions of the 30% and 60% methanol extracts of CTL were used and a 1 mg/kg of indomethacin was used as a positive anti-inflammatory standard; these were then administrated to rats. Carrageenan was injected subcutaneously to induce hind paw edema in rats. The result of carrageenan-induced rat paw oedema showed that a 1 mg/kg of the 30%, and 60% methanol fraction of CTL and 1 mg/kg of indomethacin inhibited the hind paw edema by 19.5%, 23.0%, and 20.5% respectively. The effect of CTL on inflammation in mice by a capillary permeability assay was examined by detecting Evans blue leakage from capillaries after the intraperitoneal injection of acetic acid, a potent inflammatory stimulus. The 60% methanol fraction of CTL inhibited Evans blue dye leakage by 47.0%, which was 10% higher than that of the inhibition of 1 mg/kg of indomethacin. Also, the 60% methanol fraction of CTL suppressed the prostaglandin E2 (PGE2) generation in RAW 264.7 macrophage cells after treatment with lipopolysaccharide (LPS) by as much as the inhibition of 1 mg/kg of indomethacin and this led to the synthesis of PGE2 by COX-2 induction. The inhibition of the carrageenan-induced rat paw oedema, vascular permeability and the PGE2 generation demonstrates that the 60% methanol fraction of CTL contains a potent anti-inflammatory activity.

Anti-inflammatory phenylpropanoid glycosides from Clerodendron trichotomum leaves

“…In the present study, an active fraction, 80% methanol fraction and acteoside isolated active fraction have been demonstrated to possess both in vivo and in vitro anti-inflammatory effects, suggesting that acteoside is responsible for the anti-inflammatory activity of C. trichotomum. Choi et al (2004) and Park and Kim (2007) reported that the methanol extract of C. trichotomum leaves decreased edema induced by carrageenan, acetic acid-induced capillary permeability and lipopolysaccharide (LPS)-induced PGE 2 (Choi et al, 2004;Park and Kim, 2007). Using activity-guided fractionation, C. trichotomum methanol extract was chromatographed on a column of Diaion HP-20 (Nippon Rensui Co., Tokyo, Japan) and the anti-inflammatory effect of each fraction was determined using in vitro and in vivo assays.…”

“…Choi et al (2004) reported that a methanol extract of C. trichotomum exerted an anti-inflammatory effect by suppressing the production of prostaglandin E 2 (PGE 2 ). However, nothing is known yet about the anti-inflammatory components of C. trichotomum that mediate this effect.…”

Evaluation of the in vivo activity of different concentrations of <i>Clerodendrum umbellatum</i> poir against <i>Schistosoma mansoni</i> infection in mice

“…Due to their antiparasitic property, some of these chemicals constituents could be responsible for the schistosomicidal effect of C. umbellatum leaves aqueous extract. Moreover, it has been found that some species of the genus Clerodendrum as C. petasites and C. trichotomum exhibited anti-inflammatory properties (Panthong et al, 2003;Choi et al, 2004). Given the fact that S. mansoni develops unique severe granulomatous inflammatory reactions in the liver, a plant extract with anti-inflammatory properties might be of great interest in the enhancement of the anti-inflammatory barrier.…”

Serotonergic Properties of the Roots of Clerodendron capitatum

“…The genus Clerodendron, is reported to demonstrate biological activities such as antitumorgenic [7,8] , Hypoglycemic, hypolipidemic [5,9] , Hepatoprotective activity against CCl 4 induced liver injury in rats [10,11] , Anti-inflammatory [12][13][14] , free radicalscavenging activity, Anti-diarrhoeal, Antinociceptive and antipyretic effects [15][16][17] . To the best of our knowledge, there is only one scientific report published regarding the phytomedicinal properties of CC by Adeneye et al [6] .…”