Studies on the Antagonistic Action Between Chloramphenicol and Norfloxacin by Fluorescence Spectrum

Abstract

Chloramphenicol (CHL) and Norfloxacin (NFLX) can both quench the fluorescence of bovine serum albumin (BSA) in the aqueous solution of pH ¼ 7.40. This quenching effect becomes more significant when Chloramphenicol and Norfloxacin coexist. Based on this, further studies on the interactions between Chloramphenicol and Norfloxacin using fluorescence spectrum are established. The results showed that the interaction between the drugs would increase the binding constant and binding stability of the drug and protein, thus reducing the free drug concentration. This phenomenon indicates that there exists antagonistic action between drugs. Studies utilizing synchronous spectra showed that the antagonistic action between the drugs would affect the conformation of BSA.