Studies on the Amino Acid Fermentation

Kinoshita, Shukuo; Udaka, Shigezo; Shimono, Masakazu

doi:10.2323/jgam.3.193

Cited by 482 publications

(97 citation statements)

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“…glutamicum was isolated as an L-glutamate-producing bacterium in 1956. 2,3) This was a breakthrough in the biotechnological production of amino acids by microbial fermentation. Although it was suggested that glutamate excretion was catalyzed by a specific carrier system, 15,16) the characteristics of that system were not elucidated for more than 50 years.…”

Section: Discussionmentioning

confidence: 99%

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L-Glutamate Secretion by the N-Terminal Domain of theCorynebacterium glutamicumNCgl1221 Mechanosensitive Channel

Yamashita

Hashimoto

Kumagai

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

“…In 1956, Kinoshita and coworkers isolated Corynebacterium glutamicum, originally designated Micrococcus glutamicus, as an L-glutamate-producing bacterium. 2,3) C. glutamicum is a non-sporulating, Gram-positive bacterium that has an asymmetric rod shape. The mechanism of L-glutamate secretion by C. glutamicum is unique.…”

mentioning

confidence: 99%

L-Glutamate Secretion by the N-Terminal Domain of theCorynebacterium glutamicumNCgl1221 Mechanosensitive Channel

Yamashita

Hashimoto

Kumagai

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…1,2) In addition, it is increasingly popular as a potential building block in the production of useful chemicals. Since 1957, glutamate has been industrially produced by fermentation using Corynebacterium glutamicum, 3,4) an aerobic, gram-positive bacterium.…”

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confidence: 99%

The Protein Encoded byNCgl1221inCorynebacterium glutamicumFunctions as a Mechanosensitive Channel

Hashimoto¹,

Nakamura²,

Kuroda³

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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The function of the NCgl1221-encoded protein of Corynebacterium glutamicum was analyzed using Bacillus subtilis as host because a method for preparing the giant provacuole required for electrophysiological studies has been established. Expression of NCgl1221 in a strain deficient in mscL and ykuT, both of which encode mechanosensitive channels, resulted in an 8.9-fold higher cell survival rate upon osmotic downshock than the control. Electrophysiological investigation showed that the giant provacuole prepared from this strain, expressing NCgl1221, exhibited significantly higher pressuredependent conductance than the control. These findings show that the NCgl1221-encoded protein functions as a mechanosensitive channel.

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“…glutamicum was discovered about 50 years ago. Stimulated by the increasing demand for L-glutamate as a flavor enhancer in human nutrition, a screening program in Japan led to the isolation of a soil microorganism, later classified as C. glutamicum, which was able to accumulate L-glutamate in the growth medium [1,2]. Subsequent analysis revealed that glutamate secretion could be triggered by a limited supply of biotin, opening the possibility for industrial production.…”

Section: Introductionmentioning

confidence: 99%

Analysis and Engineering of Metabolic Pathway Fluxes in Corynebacterium glutamicum

Wittmann

2010

Biosystems Engineering I

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The Gram-positive soil bacterium Corynebacterium glutamicum was discovered as a natural overproducer of glutamate about 50 years ago. Linked to the steadily increasing economical importance of this microorganism for production of glutamate and other amino acids, the quest for efficient production strains has been an intense area of research during the past few decades. Efficient production strains were created by applying classical mutagenesis and selection and especially metabolic engineering strategies with the advent of recombinant DNA technology. Hereby experimental and computational approaches have provided fascinating insights into the metabolism of this microorganism and directed strain engineering. Today, C. glutamicum is applied to the industrial production of more than 2 million tons of amino acids per year. The huge achievements in recent years, including the sequencing of the complete genome and efficient post genomic approaches, now provide the basis for a new, fascinating era of research -analysis of metabolic and regulatory properties of C. glutamicum on a global scale towards novel and superior bioprocesses.

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Studies on the Amino Acid Fermentation

Cited by 482 publications

References 0 publications

L-Glutamate Secretion by the N-Terminal Domain of theCorynebacterium glutamicumNCgl1221 Mechanosensitive Channel

L-Glutamate Secretion by the N-Terminal Domain of theCorynebacterium glutamicumNCgl1221 Mechanosensitive Channel

The Protein Encoded byNCgl1221inCorynebacterium glutamicumFunctions as a Mechanosensitive Channel

Analysis and Engineering of Metabolic Pathway Fluxes in Corynebacterium glutamicum

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