Studies on the adsorption of various proteins on the surface of polystyrene suspension particles

Nuss, P. V.; Krasheninnikova, I. G.; Grzywa-Niksinska, I.; Dorokhova, Ye.A.; Гусев, С. А.; Grzywa, E.

doi:10.14314/polimery.1995.229

Cited by 2 publications

(2 citation statements)

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“…From our results, the high Γ ads value of 8.96±0.08 mg/m 2 was obtained at pH 6.4, whereas the value was decreased to 6.54±0.09 and 6.63±0.08 mg/m 2 at pH 7.0 and 8.0, respectively. Although, at pH 6.4, the highest Γ ads value can be obtained, the prepared sensitized particle seemed to lose the stability during adsorption so called "spontaneous agglutination" which can be inhibited by increasing pH [9]. The conjugated particle adsorbed at pH 8.0 was more stable and was chosen for preparing antibodylatex conjugate.…”

Section: Preparation Of Antibody-latex Conjugatementioning

confidence: 99%

Improving Malaria Diagnosis via Latex Immunoagglutination Assay in Microfluidic Device

Thiramanas

Wanotayan

Rahong

et al. 2010

AMR

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Attempt to improve latex immunoagglutination assay, a rapid method in medical diagnostics, reporting as quantitative results was interested in this study by using microfluidic device. Sensitized latex was produced by physical adsorption of human polyclonal IgG antibody to Plasmodium falciparum malaria parasite onto carboxylated polystyrene particle. Conventional latex agglutination assay was firstly performed to verify specific interaction of antibody on the bead surface versus antigen in malaria plasma. The agglutinate size around 30 µm was observed under optical microscope. The proportion of the plasma and the particle was optimized, and an appropriate ratio was applied in microfluidic device. Three patterns of the device were used with the agglutinate size comparison after 10 min as followed: rapid mixing > U-shaped loop > straight capillary Y-junction patterns. However, compared with patient plasma, small agglutinates were also observed when using normal serum.

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Section: Preparation Of Antibody-latex Conjugatementioning

confidence: 99%

Improving Malaria Diagnosis via Latex Immunoagglutination Assay in Microfluidic Device

Thiramanas

Wanotayan

Rahong

et al. 2010

AMR

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“…The PSA latexes were chosen for our studies because, depending on the composition of the surface, on these particles proteins could be adsorbed and/or immobilized (by reacting with aldehyde groups from acrolein monomeric units). The poly(styrene/acrolein) latexes synthesized previously (17,18), were obtained by grafting polyacrolein onto the polystyrene core. Thus, their surface was composed of polyacrolein and for approaching proteins these particles were not different from the pure polyacrolein latexes.…”

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confidence: 99%

Proteins at the Interfaces of Poly(styrene—acrolein) Latexes and Solvent

Słomkowski

Kowalczyk

Basiǹska

et al. 1993

ACS Symposium Series

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The behavior of human serum albumin (HSA) and gamma globulins (γG) at the interfaces of poly(styrene/acrolein) latex particles and solvent is described. It is shown how the relative amounts of proteins bound to the latex surfaces by hydrophobic adsorption and by covalent immobilization are affected by the chemical composition of the latex particles. The competition in adsorption of "monomelic", "dimeric" and "oligomeric" HSA is also described. Studies of fluorescently marked HSA and γG at interfaces of latex particles and solvent indicate that, after attachment, protein molecules first lose their internal fluidity and only later change their conformation.In medical and veterinary applications of polymeric materials, it is essential to understand what happens to various macromolecules, vital for functions of an organism, when the macromolecules approach the polymeric surface. One of these applications entails using polymeric latexes and microspheres for diagnostic purposes. Latexes with the appropriate antibodies, adsorbed and/or covalently immobilized at their surfaces, are used for detection of the corresponding antigens in standard agglutination tests (1). Recently, the new, so called "dry tests" have been designed (e.g. the tests for detection of antigens against the HIV and hepatitis viruses (2)). In these tests the only liquid being used is the liquid from the analyzed sample.Regardless of the actual design of the test, it is crucial to establish whether the required biologically active compounds could be adsorbed or covalently immobilized at the surface of the prospective latex particles. Special attention has to be paid to the microheterogenity of the surface of polymeric composite materials, because this often leads to the distribution of the protein attachments to the patches with different chemical composition (3). Not less important is to determine the character and degree of changes occurring in the bioactive molecules upon attachment to the surface. In the 0097-6156/94/0548-0449$06.00/0

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Studies on the adsorption of various proteins on the surface of polystyrene suspension particles

Cited by 2 publications

References 0 publications

Improving Malaria Diagnosis via Latex Immunoagglutination Assay in Microfluidic Device

Improving Malaria Diagnosis via Latex Immunoagglutination Assay in Microfluidic Device

Proteins at the Interfaces of Poly(styrene—acrolein) Latexes and Solvent

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