Studies on Sweet Potato Little-Leaf Phytoplasma Detected in Sweet Potato and Other Plant Species Growing in Northern Australia

Gibb, Karen S.; Padovan, Anna; Mogen, Brad

doi:10.1094/phyto-85-169

Cited by 107 publications

(63 citation statements)

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“…A set of overlapping PCR products was generated by re-amplification of P1/P7 segment of 16S-23S rDNA segment with P1/rU3 (Deng and Hiruki 1991;Lorenz et al 1995), R16F2/R2 (Lee et al , 1995a and 16R758/P7 primers (Gibb et al 1995;Schneider et al 1995). The PCR products were obtained according to Fránová et al (2003).…”

Section: Methodsmentioning

confidence: 99%

Phytoplasma associated with witches’-broom disease of Ulmus minor Mill. in the Czech Republic: Electron microscopy and molecular characterization

et al. 2009

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Visual inspections of elm trees in south Moravia in 1997-2007 revealed a rare occurrence of plants with smaller and cowl-forming leaves on some twigs, i.e. a feature resembling witches'-broom disease observed on the end of twigs. The presence of phytoplasma-like bodies was observed by transmission electron microscopy of phloem tissue. On the other hand, no phytoplasmas were found in asymptomatic trees. Nucleic acids extracted from these plants were used in nested-PCR assays with primers amplifying 16S rRNA sequences specific for phytoplasmas. Sequence analyses of the 16S-23S ribosomal operon (1852 bp) allowed for the classification of the detected phytoplasmas in the elm yellows group, but its position remained on the boundary of the 16SrV-A and 16SrV-C ribosomal subgroups. Sequence analyses of the ribosomal protein of the rpl22-rps3 and secY genes lead to further classification and revealed the phytoplasmas' affiliations to the 'Candidates Phytoplasma ulmi'. Some exceptions in unique oligonucleotide sequences defined for 'Ca. Phytoplasma ulmi' were found in the Czech isolate. This is the northernmost confirmed occurrence of phytoplasma on elm trees within Europe.

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Section: Methodsmentioning

confidence: 99%

Phytoplasma associated with witches’-broom disease of Ulmus minor Mill. in the Czech Republic: Electron microscopy and molecular characterization

et al. 2009

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“…The tomato big bud (TBB) phytoplasma is associated with a wide range of plant diseases throughout Australia (Davis et al, 1997). The sweet potato little leaf (SPLL) phytoplasma was originally detected in, and isolated from, Ipomoea batatas growing in Northern Australia (Gibb et al, 1995). RFLP analysis of the 16S rRNA gene of TBB and SPLL phytoplasmas, isolated from their natural host plants, showed no polymorphisms (Gibb et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…The sweet potato little leaf (SPLL) phytoplasma was originally detected in, and isolated from, Ipomoea batatas growing in Northern Australia (Gibb et al, 1995). RFLP analysis of the 16S rRNA gene of TBB and SPLL phytoplasmas, isolated from their natural host plants, showed no polymorphisms (Gibb et al, 1995). TBB phytoplasma causes phyllody and an absence of normal flower production in Catharanthus roseus (periwinkle), while SPLL phytoplasma when transmitted from sweet potato to periwinkle, causes virescence and smaller flowers (Padovan et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Identification of genes in the tomato big bud phytoplasma and comparison to those in sweet potato little leaf-V4 phytoplasma

Streten¹,

Gibb²

2003

Microbiology

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Genetic relatedness of phytoplasmas is commonly defined on the basis of differences in the highly conserved 16S rRNA gene, which may not resolve closely related phytoplasmas. An example of this is the closely related tomato big bud (TBB) and sweet potato little leaf strain V4 (SPLL-V4) phytoplasmas, which cannot easily be differentiated by their 16S rRNA gene sequences. This study aimed to identify genes on the TBB phytoplasma chromosome which could be used to examine genetic variation between these two closely related phytoplasmas. Random clones generated from TBB phytoplasma genomic DNA were sequenced and characterized by database analysis. Twenty-three genes were identified within 19 random clones, which contained approximately 18?0 kbp of TBB phytoplasma genomic DNA. Half of the TBB phytoplasma genes identified were involved in DNA replication, transcription and translation. The remaining TBB phytoplasma genes were involved in protein secretion, cellular processes and energy metabolism. Phylogenetic analysis of representative genes showed that the TBB phytoplasma grouped with the mycoplasmas with the exception of the TBB phytoplasma secA gene, which grouped with the onion yellows phytoplasma. PCR primers were designed based on the new genes and tested on isolates of the TBB and SPLL-V4 phytoplasmas. Most primers amplified a product from TBB and SPLL-V4 phytoplasma samples. When amplified products were subjected to RFLP analysis, the restriction patterns were the same as the respective original clones. This result confirmed that the same sequence had been amplified by PCR and showed that these isolates were indistinguishable using the new genes. This study showed that in fact the TBB and SPLL-V4 phytoplasmas are closely related even with the analysis of new genes. These new genes have, however, provided insight into the biology of the TBB and SPLL-V4 phytoplasmas.

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“…Streten and Gibb (2006) listed 28 genera of plants as hosts of the SPLL-V4 phytoplasma in Australia. In the NT, SPLL was reported on Ipomoea batatas (sweet potato) (Gibb et al 1995, Davis et al 1997, Carica papaya (papaya) , Cleoma viscosa (mustard bush), Crotalaria brevis (rattlepod), Indigofera colutea (sticky indigo), Phyllanthus maderaspanatus and Senna obtusifolia (sicklepod) ; and SPLL-V4 was reported on Aeschynomene americana (American jointvetch), Alysicarpus vaginalis (alyce clover), Arachis hypogaea (peanut), Centrosema pascuorum (cavalcade), Crotalaria goreensis (gambia pea), Medicago sativa (lucerne), Mitracarpus hirtus (tropical girdlepod), Rhynchosia minima (rhynchosia), Sesamum indicum (sesame), Vigna radiata (mung bean) (Wilson et al 2001) and C. papaya (Padovan and Gibb 2001).…”

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confidence: 99%

Sweet potato little leaf strain V4 phytoplasma associated with snake bean in the Northern Territory, Australia

Tran-Nguyen¹,

Smith²,

Liberato³

2012

Australasian Plant Dis. Notes

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Sweet potato little leaf strain V4 phytoplasma (SPLL-V4) was found to be associated with 12-week old snake bean plants (Vigna unguiculata ssp. sesquipedalis) from a block with 890 plants in Lambells Lagoon, Northern Territory. Symptoms included witches' broom, big bud, phyllody and little leaf. There was a 19.9 % incidence of symptoms on 366 plants assessed. The same phytoplasma was also detected from a single plant of Phyllanthus amarus with witches' broom symptoms growing near the snake bean plants. The SPLL-V4 phytoplasma was identified based upon the 16S ribosomal RNA gene and intergenic spacer region nucleotide sequence and restriction fragment length polymorphism.

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Studies on Sweet Potato Little-Leaf Phytoplasma Detected in Sweet Potato and Other Plant Species Growing in Northern Australia

Cited by 107 publications

References 0 publications

Phytoplasma associated with witches’-broom disease of Ulmus minor Mill. in the Czech Republic: Electron microscopy and molecular characterization

Phytoplasma associated with witches’-broom disease of Ulmus minor Mill. in the Czech Republic: Electron microscopy and molecular characterization

Identification of genes in the tomato big bud phytoplasma and comparison to those in sweet potato little leaf-V4 phytoplasma

Sweet potato little leaf strain V4 phytoplasma associated with snake bean in the Northern Territory, Australia

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