Studies on Sulphhaemoglobin

Lemberg, R.; Holden, Henry Francis; Legge, JW; Lockwood, W. H.

doi:10.1038/icb.1942.27

Cited by 12 publications

(9 citation statements)

References 3 publications

(6 reference statements)

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“…of total blood pigment. The observation of Lemberg et al (1942) that no choleglobin was detectable in clinical sulphemoglobinemia was confirmed in the present work.…”

Section: Disappearance Of Shb In Vivosupporting

confidence: 87%

“…Some confirmation of the spectrophotometric estimates of non-oxygen combining pigment present in two bloods containing SHb was obtained by comparison of iron analyses and oxygen combining powers (Jope, Jope and O'Brien, (1944)). The spectral absorption of SHb both in the visible and UV requires further study, as the results found in this preliminary study do not agree in detail with some reported in the literature (Drabkin andAustin (1935): Michel (1938) : Lemberg et al (1942)). The Process of Formation and Nature of Sulphemoglobin By the action of H2S and 02 on hemoglobin, a compound is formed with a spectral absorption band at A = 620 m,u, the position of which is comparatively insensitive to changes in pH, and to many reagents such as KCN or ammonium sulphide, which affect the spectrum of MHb.…”

contrasting

confidence: 90%

“…The change in absorption spectrum indicates that the chief differences between SHb and HbO2 may be expected in the heme group. It seems clear now that the prophyrin ring structure is unbroken in SHb (Lemberg et al (1942): Jope (1941)). Haurowitz (1940) found that acid hydrolysis or peptic digestion of SHb did not give a separation of the heme group from the protein as it did with hemoglobin itself, but gave a compound with much protein material still attached to the heme, which he called Sulpheminproteose.…”

mentioning

confidence: 99%

“…The process of SHb formation in vivo is not known, though it probably involves an oxidation and Michel (1938) The constitution of SHb is still uncertain, and many of the difficulties in elucidating it arise from the inability to produce the pigment in pure form (Lemberg et al (1942) ). That the general character -of the protein in SHb is the same as that of the oxyhemoglobin from which it is formed is indicated by the electrophoretic homogeneity of the two pigments as shown by Michel (1938), and it has been found impossible so far to separate them by chromatography (Jope, Jope and O'Brien (1944) ).…”

mentioning

confidence: 99%

“…That the general character -of the protein in SHb is the same as that of the oxyhemoglobin from which it is formed is indicated by the electrophoretic homogeneity of the two pigments as shown by Michel (1938), and it has been found impossible so far to separate them by chromatography (Jope, Jope and O'Brien (1944) ). Michel (1938) found it impossible to separate rat or dog SHb from oxyhemoglobin by crystallization, though Lemberg et al (1942) have achieved such a separation in the case of SHb formed by sulphur and acetanilide ingestion in rats. The change in absorption spectrum indicates that the chief differences between SHb and HbO2 may be expected in the heme group.…”

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confidence: 99%

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Disappearance of Sulphemoglobin from the Blood of T.N.T. Workers in Relation to the Dynamics of Red Cell Destruction

Jope¹

1946

Occupational and Environmental Medicine

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Preliminary studies of the rates of disappearanc of Methemoglobin (MHb) and Sulphemoglobi (SHb) from human blood in vivo after cessatio of contact with the agents, such as sulphonamide: acetanilide or TNT, causing these abnormal piE ments : Jope, O'Brie and Watson (1942)), revealed a striking contra: between MHb and SHb in this respect. MHb wa found to disappear rapidly from the circulatin blood and was undetectable after 2 to 5 days fror removal of the causative agent (cf. also Cox an Wendel (1942)), whereas SHb was found to persi and was still detectable after many weeks. Meth4 moglobin also disappeared rapidly from draw blood, being usually no longer detectable after aboi 24 hours at room temperature (Jope and O'Brie (1942) (1930, 1931Wendel (1933): Cox and Wendel (1942): and Dral kin (1945 ). SHb, on the other hand, may be kel unchanged in drawn blood even longer than the re cells can be kept intact, and remains for sever, weeks after hemolysis as a constant fraction of ti total pigment present, so long in fact as the bloc can be kept sterile. It appears, therefore, that ti red cell has no mechanism within it for the tran formation of SHb. If this is so, the body can ha) no means of removing SHb other than by destru tion of the red cells that contain it.The disappearance of sulphemoglobin in vil was therefore studied in some detail, both in ord4 to gain a more precise knowledge of the conditioi necessary for recovery from SHb cyanoses, and as possible means of studying the dynamics of red cc destruction.

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“…of total blood pigment. The observation of Lemberg et al (1942) that no choleglobin was detectable in clinical sulphemoglobinemia was confirmed in the present work.…”

Section: Disappearance Of Shb In Vivosupporting

confidence: 87%

contrasting

confidence: 90%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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