Studies on Succinic Dehydrogenase

Changes in the chemistry of an isoalloxazine brought about by substitution at the 7 and 8 positions by a strongly electronegative substituent

“…After 15 min of stirring the mixture was extracted five times with 200 mL of dichloromethane. The organic extracts were combined, dried (MgS04), and evaporated to yield a light yellow oil which was distilled (88-89 °C (0.15 mm)) to yield 9.77 g (96%) of XIV: IR 2160 cm-1 (C=N), no carbonyl stretch; NMR (CDCI3, Me4Si) 5 2.79 (s, 3 ), 4.13 (s, 1 , NH), 6.6-7.4 (m, 4 H).…”

Complex II ambiguities—FADH2 in the electron transfer system

“…In most flavin-linked dehydrogenases the flavin adenine nucleotide is a tightly bound prosthetic group. In CII, it is even covalently and thus permanently bound to the enzyme during the catalytic cycle when the redox state is regenerated in each enzymatic turnover, as documented in early reports ( 35 ) and summarized in classical textbooks ( 36 , 37 ). Structural studies of CII have expanded our knowledge of the mechanism of enzyme assembly ( 13 ), enzyme structure ( 38 , 39 , 40 ), kinetic regulation of CII activity ( 41 , 42 ), and associated pathologies ( 3 , 26 , 27 , 28 , 29 ).…”