Studies on some biochemical characteristics of Staphylococcus aureus of buffalo mammary origin

Varshney, J. P.; Kapur, M. P.; Sharma, Anshu

doi:10.1016/0147-9571(93)90161-w

Comparative Immunology, Microbiology and Infectious Diseases

1993

DOI: 10.1016/0147-9571(93)90161-w

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Studies on some biochemical characteristics of Staphylococcus aureus of buffalo mammary origin

J. P. Varshney

M. P. Kapur

Anshu Sharma

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2010

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“…Therefore, presence of beta-haemolysin on blood agar in combination with a positive coagulase activity seems to represent the optimal criterion for the identification of Staph. aureus from mastitis milk samples (Varshney et al 1993; Lam et al 1995; Boerlin et al 2003). Additionally, testing for the presence of surface proteins, clumping factor (CF) and/or protein-A, are helpful in routine analysis for the rapid identification of Staph.…”

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A coagulase-negative variant ofStaphylococcus aureusfrom bovine mastitis milk

Akineden

Hassan

Schneider

et al. 2010

Journal of Dairy Research

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Bacteriological analysis of milk samples from quarters of a dairy cow suffering from subclinical mastitis yielded two isolates of Staphylococcus aureus which gave a negative reaction in the standard coagulase test. Both isolates were also clumping factor and thermonuclease negative, and gave a negative reaction in the Staphaurex® test. The isolates were identified by using commercial biochemical systems, and by PCR analysis of different staphylococcal cell surface protein and exoprotein genes. Further molecular identification of the isolates, which included sequencing of the 16S rRNA gene and RT-PCR of coagulase (coa), clumping-factor (clfA) and thermonuclease (nuc) genes, was consistent with the diagnosis phenotypically 'coagulase-negative variant of Staph. aureus'. The fact that coagulase-negative Staph. aureus variants can occur in the context of intramammary infections in cattle may result in the incorrect diagnosis 'coagulase-negative staphylococci (CNS)' in routine mastitis diagnostic, at least in rare cases. To fully ensure correct species diagnosis, sequencing of the 16S rRNA gene and amplification of specific genes such as coa is necessary in these cases.

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confidence: 99%

A coagulase-negative variant ofStaphylococcus aureusfrom bovine mastitis milk

Akineden

Hassan

Schneider

et al. 2010

Journal of Dairy Research

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Studies on some biochemical characteristics of Staphylococcus aureus of buffalo mammary origin

Cited by 1 publication

References 18 publications

A coagulase-negative variant ofStaphylococcus aureusfrom bovine mastitis milk

A coagulase-negative variant ofStaphylococcus aureusfrom bovine mastitis milk

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