Studies on reactions of oxidizing sulfur–sulfur three-electron-bond complexes and reducing α-amino radicals derived from OH reaction with methionine in aqueous solution

Lu, Can‐Zhong; Yao, Side; Lin, Nianyun

doi:10.1016/s0304-4165(00)00174-4

Cited by 23 publications

(19 citation statements)

References 46 publications

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“…Sinapic acid is a cinnamic acid derivative, and it possesses 3,5-dimethoxy and 4-hydroxyl substitutions in the phenyl group of cinnamic acid. Sinapic acid is widely distributed in the plant kingdom and can be obtained from various sources, such as rye, broccoli, cabbage, and kale [42]. Sinapic acid has already been pharmacologically evaluated for its potent antioxidant, anxiolytic, anti-inflammatory, and peroxynitrite scavenging effects and neuroprotective effects [43].…”

Section: Resultsmentioning

confidence: 99%

Binary Solvent Extraction System and Extraction Time Effects on Phenolic Antioxidants from Kenaf Seeds (Hibiscus cannabinusL.) Extracted by a Pulsed Ultrasonic-Assisted Extraction

Wong

Lau

Tan

et al. 2014

The Scientific World Journal

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The aim of this study was to determine the best parameter for extracting phenolic-enriched kenaf (Hibiscus cannabinus L.) seeds by a pulsed ultrasonic-assisted extraction. The antioxidant activities of ultrasonic-assisted kenaf seed extracts (KSE) were determined by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay, β-carotene bleaching inhibition assay, and ferric reducing antioxidant power (FRAP) assay. Total phenolic content (TPC) and total flavonoid content (TFC) evaluations were carried out to determine the phenolic and flavonoid contents in KSE. The KSE from the best extraction parameter was then subjected to high performance liquid chromatography (HPLC) to quantify the phenolic compounds. The optimised extraction condition employed 80% ethanol for 15 min, with the highest values determined for the DPPH, ABTS, and FRAP assay. KSE contained mainly tannic acid (2302.20 mg/100 g extract) and sinapic acid (1198.22 mg/100 g extract), which can be used as alternative antioxidants in the food industry.

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Section: Resultsmentioning

confidence: 99%

Binary Solvent Extraction System and Extraction Time Effects on Phenolic Antioxidants from Kenaf Seeds (Hibiscus cannabinusL.) Extracted by a Pulsed Ultrasonic-Assisted Extraction

Wong

Lau

Tan

et al. 2014

The Scientific World Journal

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“…Met is one of the most easily oxidized amino acids by ROS, being converted to methionine sulfoxide (MetO) [ 72 ]. Free Met molecules, as well as Met residues in proteins, are efficient scavengers of almost all oxidizing molecules, such as H 2 O 2 , hydroxyl radical, peroxynitrite and hypochlorous acid [ 73 , 74 , 75 ]. A non-polar S-methyl thioeter side chain of Met is almost always situated in the interior hydrophobic core of globular proteins.…”

Section: Discussionmentioning

confidence: 99%

Alterations in the Antioxidant Enzyme Activities in the Neurodevelopmental Rat Model of Schizophrenia Induced by Glutathione Deficiency during Early Postnatal Life

et al. 2020

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The aim of the present study was to assess the effects of l-buthionine-(S,R)-sulfoximine (BSO), a glutathione (GSH) synthesis inhibitor, and GBR 12909, a dopamine reuptake inhibitor, administered alone or in combination to Sprague-Dawley rats during early postnatal development (p5–p16), on the levels of reactive oxygen species (ROS), lipid peroxidation (LP) and the activities of antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione disulfide reductase (GR) in peripheral tissues (liver, kidney) and selected brain structures (prefrontal cortex, PFC; hippocampus, HIP; and striatum, STR) of 16-day-old rats. The studied parameters were analyzed with reference to the content of GSH and sulfur amino acids, methionine (Met) and cysteine (Cys) described in our previous study. This analysis showed that treatment with a BSO + GBR 12909 combination caused significant decreases in the lipid peroxidation levels in the PFC and HIP, in spite of there being no changes in ROS. The reduction of lipid peroxidation indicates a weakening of the oxidative power of the cells, and a shift in balance in favor of reducing processes. Such changes in cellular redox signaling in the PFC and HIP during early postnatal development may result in functional changes in adulthood.

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“…Therefore, the formation of an intermediate with a two-center three-electron bond between two sulfur atoms is very favorable ( Fig. 1 ) [5] . Further oxidation of the three-electron (S–S) intermediate requires significantly less energy than oxidation of free cysteine, therefore, oxidation of cysteine residues in the ZnS 4 cluster can occur even under conditions of mild oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Protein engineering to develop a redox insensitive endothelial nitric oxide synthase

et al. 2014

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The zinc tetrathiolate (ZnS4) cluster is an important structural feature of endothelial nitric oxide synthase (eNOS). The cluster is located on the dimeric interface and four cysteine residues (C94 and C99 from two adjacent subunits) form a cluster with a Zn ion in the center of a tetrahedral configuration. Due to its high sensitivity to oxidants this cluster is responsible for eNOS dimer destabilization during periods of redox stress. In this work we utilized site directed mutagenesis to replace the redox sensitive cysteine residues in the ZnS4 cluster with redox stable tetra-arginines. Our data indicate that this C94R/C99R eNOS mutant is active. In addition, this mutant protein is insensitive to dimer disruption and inhibition when challenged with hydrogen peroxide (H2O2). Further, the overexpression of the C94R/C99R mutant preserved the angiogenic response in endothelial cells challenged with H2O2. The over-expression of the C94R/C99R mutant preserved the ability of endothelial cells to migrate towards vascular endothelial growth factor (VEGF) and preserved the endothelial monolayer in a scratch wound assay. We propose that this dimer stable eNOS mutant could be utilized in the treatment of diseases in which there is eNOS dysfunction due to high levels of oxidative stress.

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Studies on reactions of oxidizing sulfur–sulfur three-electron-bond complexes and reducing α-amino radicals derived from OH reaction with methionine in aqueous solution

Cited by 23 publications

References 46 publications

Binary Solvent Extraction System and Extraction Time Effects on Phenolic Antioxidants from Kenaf Seeds (Hibiscus cannabinusL.) Extracted by a Pulsed Ultrasonic-Assisted Extraction

Binary Solvent Extraction System and Extraction Time Effects on Phenolic Antioxidants from Kenaf Seeds (Hibiscus cannabinusL.) Extracted by a Pulsed Ultrasonic-Assisted Extraction

Alterations in the Antioxidant Enzyme Activities in the Neurodevelopmental Rat Model of Schizophrenia Induced by Glutathione Deficiency during Early Postnatal Life

Protein engineering to develop a redox insensitive endothelial nitric oxide synthase

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