Studies on Production of Antipoliomyelitis Serum in Rabbits.

Levinson, Sidney O.; Milzer, Albert; Shaughnessy, Howard J.; Wolf, Albert M.; Janota, M; Vanderboom, Katherine; Neal, John L.; Morrissey, Richard A.

doi:10.3181/00379727-87-21303

Cited by 6 publications

(2 citation statements)

References 3 publications

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“…Hyperimmune sera of various sources have been used for typing, but the majority of the strains were typed against rabbit sera prepared in our lab oratory, tissue culture fluid being used as antigen for the immunization of the rabbits (8,10).…”

Section: Methodsmentioning

confidence: 99%

Some Observations on the Value of Virus Isolations and Serological Tests for the Routine Diagnosis of Neurotropic Virus Infections – Slovenian Experience

Likar¹

1958

Pathobiology

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Section: Methodsmentioning

confidence: 99%

Some Observations on the Value of Virus Isolations and Serological Tests for the Routine Diagnosis of Neurotropic Virus Infections – Slovenian Experience

Likar¹

1958

Pathobiology

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“…The actual weight of inactivated virus injected into each rabbit was, however, only about 50jtg., of which only an unknown proportion consisted of immunizing antigen. In the same year Levinson, Milzer, Shaughnessy, Neal & Oppenheimer (1945) reported a new method for the production of potent inactivated vaccines with ultra-violet irradiation. Technical details were not available at that time, but bacteria and viruses were described as inactivated 'in a fraction of a second by exposing continuously flowing thin films with a depth of less than 1 mm.…”

Section: Introductionmentioning

confidence: 99%

The Antigenicity of Ultra-Violet Irradiated Vaccinia Virus

1955

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SummaryVaccinia virus which has been inactivated by exposure to ultra-violet irradiation under strictly controlled conditions produces an immunity response in rabbits and monkeys. This can be measured by relative insusceptibility to challenge with living virus and by the titration of circulating antibody.Experiments with small doses of living virus in excess of those that might have been given accidentally indicate that the immune response to the irradiated material was not due to traces of living virus that had escaped detection.There is a logarithmic relationship between exposure to irradiation and destruction of virus. The exposure necessary to produce complete inactivation can be deduced from a knowledge of this relationship. Excessive exposure destroys the antigenicity of the preparations.The antigenicity of irradiated virus can be preserved for at least 6 months at 4°C. and at least 2 months at 37°C. by drying from the frozen state.Circulating antibody is detectable in rabbits for at least 22 weeks following immunization, and a single subsequent reinforcing dose is followed by a secondary immune response.The possibility that preliminary immunization with irradiated virus may diminish the risk of complications and reduce the severity of the reaction to subsequent Jennerian vaccination with living virus is discussed. This material may provide a basal immunity in those individuals in which vaccination is contraindicated.

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Designing a Vaccine Against HIV

Schultz¹

1999

Vaccines

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Studies on Production of Antipoliomyelitis Serum in Rabbits.

Cited by 6 publications

References 3 publications

Some Observations on the Value of Virus Isolations and Serological Tests for the Routine Diagnosis of Neurotropic Virus Infections – Slovenian Experience

Some Observations on the Value of Virus Isolations and Serological Tests for the Routine Diagnosis of Neurotropic Virus Infections – Slovenian Experience

The Antigenicity of Ultra-Violet Irradiated Vaccinia Virus

Designing a Vaccine Against HIV

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