Studies on prodigiosin and the bipyrrole precursor

Wasserman, Harry H.; McKeon, James E.; Smith, Lewis; Forgione, Peter

doi:10.1016/s0040-4020(01)90965-2

Cited by 38 publications

(16 citation statements)

References 11 publications

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“…Materials-Prodigiosin was prepared from the culture broth of Serratia marsescens as described (10) and metacycloprodigiosin and prodigiosin 25-C were obtained from Streptomyces hiroshimensis (11). Rats (Wistar, male) were obtained from Sankyo Labo Service (Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Prodigiosins as a New Group of H+/Cl−Symporters That Uncouple Proton Translocators

Satō¹,

Konno²,

Tanaka³

et al. 1998

Journal of Biological Chemistry

158

113

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We reported previously (Kataoka, T., Muroi, M., Ohkuma, S., Waritani, T., Magae, J., Takatsuki /Cl؊ symport (or OH ؊ /Cl ؊ exchange, in its equivalence) across vesicular membranes. In fact, prodigiosins displayed H ؉ /Cl ؊ symport activity on liposomal membranes. First of all, they decreased the internal pH of liposomes depending on the external chloride, and raised it depending on the internal chloride when external buffer was free from chloride. Second, their effect was electroneutral and not seriously affected by the application of an inside positive membrane potential generated by K ؉ and valinomycin. Finally, they promoted the uptake of [ 36 Cl] from external buffers with concomitant intraliposomal acidification when external pH was acidic relative to liposome interior. As prodigiosins hardly inhibit the catalytic activity (ATP hydrolysis) unlike well known OH ؊ /Cl ؊ exchangers (for example, tributyltin chloride), they should provide powerful tools for the study of molecular machinery and cellular activities involving transport of protons and/or chloride.

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Section: Methodsmentioning

confidence: 99%

Prodigiosins as a New Group of H+/Cl−Symporters That Uncouple Proton Translocators

Satō¹,

Konno²,

Tanaka³

et al. 1998

Journal of Biological Chemistry

158

113

View full text Add to dashboard Cite

show abstract

“…The bcc and fcc nuclei form from MRO clusters in several steps: (i) a thin platelet of MRO appears first; (ii) it grows to form three dimensional MRO clusters; (iii) crystal embryos form in these clusters; finally (iv) crystal embryos transform into nuclei of the stable crystalline phase. The authors also identified the mechanisms by which bcc precursors assist in the formation of fcc nuclei: it was found that (112) surfaces and steps on (110) surfaces of the bcc precursors are energetically favorable for initiating the nucleation of the fcc phase, a phenomenon reflected in the specific orientational relationships observed between the fcc and bcc structures (Pitsch, Nishiyama-Wassermann [204] and Kurdjumov-Sachs [205] relationships). With increasing reduced temperature the following structural sequences were observed during nucleation ( Fig.…”

Section: Competing Fcc and Bcc Nucleationmentioning

confidence: 99%

Phase-field modeling of crystal nucleation in undercooled liquids – A review

Gránásy

Tóth

Warren

et al. 2019

Progress in Materials Science

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We review how phase-field models contributed to the understanding of various aspects of crystal nucleation including homogeneous and heterogeneous processes, and their role in microstructure evolution. We recall results obtained both by the conventional phase-field approaches that rely on spatially averaged (coarse grained) order parameters in capturing freezing, and by the recently developed phase-field crystal models that work on the molecular scale, while employing time averaged particle densities, and are regarded as simple dynamical density functional theories of classical particles. Besides simpler cases of homogeneous and heterogeneous nucleation, phenomena addressed by these techniques include precursor assisted nucleation, nucleation in eutectic and phase separating systems, phase selection via competing nucleation processes, growth front nucleation (a process, in which grains of new orientations form at the solidification front) yielding crystal sheaves and spherulites, and transition between the growth controlled cellular and the nucleation dominated equiaxial solidification morphologies. * granasy.laszlo@wigner.mta.hu arXiv:1907.05732v1 [cond-mat.mtrl-sci] 12 Jul 2019 D. Discussion: Nucleation in coarse grained PF models 32 IV. Molecular scale phase-field models of crystallization 34 A. The Phase-Field Crystal approach 34 1. The single-mode PFC model 34 2. The two-mode PFC model 35 3. The multi-mode PFC model 36 4. Other advanced PFC models 36 B. Application of the PFC models to crystal nucleation 37 1. Euler-Lagrange equation and other methods to find free energy extrema 37 2. PFC with diffusive dynamics (DPFC) 39 3. Hydrodynamic PFC model of freezing (HPFC) 46 4. Numerical methods 49 C.

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“…Test results were read after incubation of the co-inoculated PG plate at 30 C for 2-3 days. MBC used for chemical reference was extracted from mutant 933, purified and identified as described previously (24).…”

Section: Bacterialmentioning

confidence: 99%

Identification and Characterization of the pswP Gene Required for the Parallel Production of Prodigiosin and Serrawettin W1 in Serratia marcescens

Sunaga

Sato

et al. 2004

Microbiology and Immunology

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Serratia marcescens mutants defective in production of the red pigment prodigiosin and the biosurfactant serrawettin W1 in parallel were isolated by transposon mutagenesis of strain 274. Cloning of the DNA fragment required for production of these secondary metabolites with different chemical structures pointed out a novel open reading frame (ORF) named pswP. The putative product PswP (230 aa) has the distinct signature sequence consensus among members of phosphopantetheinyl transferase (PPTase) which phosphopantetheinylates peptidyl carrier protein (PCP) mostly integrated in the nonribosomal peptide synthetases (NRPSs) system. Since serrawettin W1 belongs to the cyclodepsipeptides, which are biosynthesized through the NRPSs system, and one pyrrole ring in prodigiosin has been reported as a derivative of L‐proline tethered to phosphopantetheinylated PCP, the mutation in the single gene pswP seems responsible for parallel failure in production of prodigiosin and serrawettin W1.

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Studies on prodigiosin and the bipyrrole precursor

Cited by 38 publications

References 11 publications

Prodigiosins as a New Group of H+/Cl−Symporters That Uncouple Proton Translocators

Prodigiosins as a New Group of H+/Cl−Symporters That Uncouple Proton Translocators

Phase-field modeling of crystal nucleation in undercooled liquids – A review

Identification and Characterization of the pswP Gene Required for the Parallel Production of Prodigiosin and Serrawettin W1 in Serratia marcescens

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