Studies on Polynucleotide-Stimulated Amino Acyl Transfer From Soluble-Rna to Rat Liver Ribosomes

Fessenden, June M.; Cairncross, Judith; Moldave, Kivie

doi:10.1073/pnas.49.1.82

Cited by 9 publications

(1 citation statement)

References 27 publications

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“…The translation elongation factors (EFs) are a set of proteins responsible for translation elongation during protein synthesis ( Fessenden et al, 1963 ). There are three EFs in eukaryotic cells: EF-1, EF-2, and EF-3.…”

Section: Introductionmentioning

confidence: 99%

The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1A

Zhu

Gao

et al. 2021

Front. Microbiol.

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Middle East respiratory syndrome coronavirus (MERS-CoV), a pathogen causing severe respiratory disease in humans that emerged in June 2012, is a novel beta coronavirus similar to severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, immunoprecipitation and proximity ligation assays revealed that the nucleocapsid (N) protein of MERS-CoV interacted with human translation elongation factor 1A (EF1A), an essential component of the translation system with important roles in protein translation, cytokinesis, and filamentous actin (F-actin) bundling. The C-terminal motif (residues 359–363) of the N protein was the crucial domain involved in this interaction. The interaction between the MERS-CoV N protein and EF1A resulted in cytokinesis inhibition due to the formation of inactive F-actin bundles, as observed in an in vitro actin polymerization assay and in MERS-CoV-infected cells. Furthermore, the translation of a CoV-like reporter mRNA carrying the MERS-CoV 5′UTR was significantly potentiated by the N protein, indicating that a similar process may contribute to EF1A-associated viral protein translation. This study highlights the crucial role of EF1A in MERS-CoV infection and provides new insights into the pathogenesis of coronavirus infections.

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Section: Introductionmentioning

confidence: 99%