Studies on poly-3-hydroxyoctanoate biosynthesis by a consortium of microorganisms

Eremia, Mihaela Carmen; Lupescu, Irina; Vladu, Mariana Gratiela; Petrescu, Maria; Savoiu, Gabriela; Stefaniu, Amalia; Spiridon, Maria

doi:10.1515/auoc-2016-0009

Cited by 3 publications

(2 citation statements)

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“…For the accumulation of poly-3-hydroxyoctanoate (PHO) [68], batch fermentation production was studied using a consortium of bacterial strains Pseudomonas putida and Bacillus subtilis/Bacillus subtilis BSV in a ratio of 3:1, compared to PHO biosynthesis with P. putida (Table 3, Figure 4). For the accumulation of poly-3-hydroxyoctanoate (PHO) [68], ba production was studied using a consortium of bacterial strains Pseudo Bacillus subtilis/Bacillus subtilis BSV in a ratio of 3:1, compared to PHO P. putida (Table 3, Figure 4). The results revealed the consortium performance regarding the pro ymers (%): 85.83-86.8 C8, 5.38-5.55 C6, and 5.65-6.45 C10 were obtained…”

Section: Fermentation Bioprocessmentioning

confidence: 99%

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Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization

2022

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible biopolymers. These biomaterials have grown in importance in the fields of tissue engineering and tissue reconstruction for structural applications where tissue morphology is critical, such as bone, cartilage, blood vessels, and skin, among others. Furthermore, they can be used to accelerate the regeneration in combination with drugs, as drug delivery systems, thus reducing microbial infections. When cells are cultured under stress conditions, a wide variety of microorganisms produce them as a store of intracellular energy in the form of homo- and copolymers of [R]—hydroxyalkanoic acids, depending on the carbon source used for microorganism growth. This paper gives an overview of PHAs, their biosynthetic pathways, producing microorganisms, cultivation bioprocess, isolation, purification and characterization to obtain biomaterials with medical applications such as tissue engineering.

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Section: Fermentation Bioprocessmentioning

confidence: 99%

“…Fed-batch fermentation for PHA biosynthesis. Reprinted with permission from ref [68],. 2016, Eremia et al, Ovidius Univ.…”

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Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization

2022

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Recent Progress in Polyhydroxyalkanoates‐Based Copolymers for Biomedical Applications

Luo

Liu

et al. 2019

Biotechnology Journal

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In recent years, naturally biodegradable polyhydroxyalkanoate (PHA) monopolymers have become focus of public attentions due to their good biocompatibility. However, due to its poor mechanical properties, high production costs, and limited functionality, its applications in materials, energy, and biomedical applications are greatly limited. In recent years, researchers have found that PHA copolymers have better thermal properties, mechanical processability, and physicochemical properties relative to their homopolymers. This review summarizes the synthesis of PHA copolymers by the latest biosynthetic and chemical modification methods. The modified PHA copolymer could greatly reduce the production cost with elevated mechanical or physicochemical properties, which can further meet the practical needs of various fields. This review further summarizes the broad applications of modified PHA copolymers in biomedical applications, which might shred lights on their commercial applications.

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The Production of Biodegradable Polymers-medium-chain-length Polyhydroxyalkanoates (mcl-PHA) in Pseudomonas putida for Biomedical Engineering Applications

Ené

Vladu

Lupescu

et al. 2022

CPB

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Background: Polyhydroxyalkanoates (PHAs) are bacteria-synthetized biopolymers under unbalanced growth conditions. These biopolymers are considered potential biomaterials for future applications for their biocompatibility and biodegradable features and potential biomaterials for future applications for their biocompatibility and biodegradable characteristics and their ability to be quickly produced and functionalize with strong mechanical resistance. This article is intended to perform microbial fermentation using Pseudomonas putida strain to show the amount of biopolymers of the type polyhydroxyalkanoates with medium-chain-length (mcl-PHA) obtained depending on the type and quantity of added precursors (glucose and fatty acids). Methods: It is important to understand the microbial interaction and mechanism involved in PHA biosynthetis.For these, several methods were used, such as: obtaining microbial biomass by using a Pseudomonas putida strain able of PHA-producing, analysis of biopolymer production by acetone extraction following the Soxhlet method, purification of biopolymer by methanol-ethanol treatment, followed by the estimation of biomass by spectrophotometric analysis and the measurement of the dry weight of cells and the quantification of the amount of biopolymer produced following the gas chromatographic method (GC). Results: The highest PHA yield was obtained using octanoic (17 mL in 2000 mL medium) and hexanoic acids (14 mL in 2000 mL medium) as precursors. Consequently, octanoic acid – octanoic acid, heptanoic acid – nonanoic acid, and octanoic acid - hexanoic acid were the mix of precursors that supported the amount of PHA obtained. Conclusion: Of the 4 types of structurally related substrate, the strain Pseudomonas putida ICCF 319 prefers the C8 sublayer for an elastomeric PHA's biosynthesis with a composition in which the C8 monomer predominates over C6 and C10.

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Studies on poly-3-hydroxyoctanoate biosynthesis by a consortium of microorganisms

Cited by 3 publications

References 16 publications

Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization

Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization

Recent Progress in Polyhydroxyalkanoates‐Based Copolymers for Biomedical Applications

The Production of Biodegradable Polymers-medium-chain-length Polyhydroxyalkanoates (mcl-PHA) in Pseudomonas putida for Biomedical Engineering Applications

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