Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization

Miu, Dana-Maria; Eremia, Mihaela Carmen; Moscovici, Mișu

doi:10.3390/ma15041410

Cited by 28 publications

(14 citation statements)

References 178 publications

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“…Polyhydroxyalkanoates (PHAs) have been identified as biocompatible and biodegradable biopolymers and that are produced by a range of bacteria serving as energy and carbon storage materials (74). PHAs have drawn a lot of attention because of their potential as particulate vaccines in delivering various antigens from diverse pathogens (75).…”

Section: Polymeric Vaccinesmentioning

confidence: 99%

Precision-engineering of subunit vaccine particles for prevention of infectious diseases

et al. 2023

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Vaccines remain the best approach for the prevention of infectious diseases. Protein subunit vaccines are safe compared to live-attenuated whole cell vaccines but often show reduced immunogenicity. Subunit vaccines in particulate format show improved vaccine efficacy by inducing strong immune responses leading to protective immunity against the respective pathogens. Antigens with proper conformation and function are often required to induce functional immune responses. Production of such antigens requiring post-translational modifications and/or composed of multiple complex domains in bacterial hosts remains challenging. Here, we discuss strategies to overcome these limitations toward the development of particulate vaccines eliciting desired humoral and cellular immune responses. We also describe innovative concepts of assembling particulate vaccine candidates with complex antigens bearing multiple post-translational modifications. The approaches include non-covalent attachments (e.g. biotin-avidin affinity) and covalent attachments (e.g. SpyCatcher-SpyTag) to attach post-translationally modified antigens to particles.

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Section: Polymeric Vaccinesmentioning

confidence: 99%

Precision-engineering of subunit vaccine particles for prevention of infectious diseases

et al. 2023

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Section: Common Types Of Pha Used In Tissue Repair and Engineeringmentioning

confidence: 99%

“…In the last decade, PHAs have drawn substantial interest for tissue engineering and as biomaterials for scaffolds, due to their thermal and mechanical properties, as well as biocompatibility and biodegradability, and feasibility to blend with other polymers to form copolymers [ 28 , 29 , 30 , 31 , 32 ]. The U.S. Food and Drug Administration (FDA) has approved PHAs for several biomedical applications [ 28 , 31 ]. Hence, the polymer has been widely investigated for artificial cartilages, dressing of wounds, artificial vessels, tendon healing, tubular substitutes, heart valves, bone grafts and stents for nerve repair [ 28 , 29 , 30 , 31 , 32 , 33 , 34 ], as summarized in Table 1 .…”

Section: Common Types Of Pha Used In Tissue Repair and Engineeringmentioning

confidence: 99%

“…The U.S. Food and Drug Administration (FDA) has approved PHAs for several biomedical applications [ 28 , 31 ]. Hence, the polymer has been widely investigated for artificial cartilages, dressing of wounds, artificial vessels, tendon healing, tubular substitutes, heart valves, bone grafts and stents for nerve repair [ 28 , 29 , 30 , 31 , 32 , 33 , 34 ], as summarized in Table 1 . In fact, up to date, the US FDA has approved a number of PHA-based products, including TephaFLEX ® (sutures made of poly (4-hydroxybutyrate), P(4HB)) [ 31 , 35 ], Phasix TM (mesh made from P(4HB) for the treatment of hernia) [ 31 , 36 ] and Monomax ® (sutures made of P(4HB)) [ 31 , 37 ].…”

Section: Common Types Of Pha Used In Tissue Repair and Engineeringmentioning

confidence: 99%

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Biomedical Applications of Polyhydroxyalkanoate in Tissue Engineering

et al. 2022

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Tissue engineering technology aids in the regeneration of new tissue to replace damaged or wounded tissue. Three-dimensional biodegradable and porous scaffolds are often utilized in this area to mimic the structure and function of the extracellular matrix. Scaffold material and design are significant areas of biomaterial research and the most favorable material for seeding of in vitro and in vivo cells. Polyhydroxyalkanoates (PHAs) are biopolyesters (thermoplastic) that are appropriate for this application due to their biodegradability, thermo-processability, enhanced biocompatibility, mechanical properties, non-toxicity, and environmental origin. Additionally, they offer enormous potential for modification through biological, chemical and physical alteration, including blending with various other materials. PHAs are produced by bacterial fermentation under nutrient-limiting circumstances and have been reported to offer new perspectives for devices in biological applications. The present review discusses PHAs in the applications of conventional medical devices, especially for soft tissue (sutures, wound dressings, cardiac patches and blood vessels) and hard tissue (bone and cartilage scaffolds) regeneration applications. The paper also addresses a recent advance highlighting the usage of PHAs in implantable devices, such as heart valves, stents, nerve guidance conduits and nanoparticles, including drug delivery. This review summarizes the in vivo and in vitro biodegradability of PHAs and conducts an overview of current scientific research and achievements in the development of PHAs in the biomedical sector. In the future, PHAs may replace synthetic plastics as the material of choice for medical researchers and practitioners.

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“…Therefore, the development of biodegradable and recyclable polymers having the desirable material properties required in practical use has provided a partial solution for the current plastics pollution . In this context, polyhydroxyalkanoates (PHAs), a unique class of intracellular polyesters naturally produced by bacterial fermentation, feature excellent biocompatibility and biodegradability, − but without the erosion of mechanical properties in comparison with that of traditional petroleum-derived plastics, which allows PHAs to be widely used in the industry, agriculture, packaging, and pharmaceutical fields. , Currently, poly(3-hydroxybutyrate) (P3HB) is the most prominent and extensively studied PHAs, and it features a perfectly stereoregular structure and is used as a crystalline thermoplastic material . Although P3HB exhibits comparable mechanical properties, UV resistance, and oxygen permeability with isotactic polypropylene, the inherent brittleness, poor elasticity, and high degree of crystallinity impede its widespread application as renewable and biodegradable materials …”

Section: Introductionmentioning

confidence: 99%

Toughening Poly(3-hydroxybutyrate) by Using Catalytic Carbonylative Terpolymerization of Epoxides

Yang

Zhang

et al. 2023

Macromolecules

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The development of biodegradable and recyclable polymers having the desired material properties required in practical use has provided a partial solution for realizing a sustainable plastics economy. Naturally produced commercial poly(3-hydroxybutyrate) (P3HB), one of the much studied biodegradable polyhydroxyalkanoates (PHAs), is a stereoregular thermoplastic material with a high degree of crystallinity, significantly restricting its application due to the stiff and brittle characteristics. Therefore, it is of great significance to overcome the inherent brittleness through decreasing the crystallinity of P3HB. In this contribution, we present a powerful strategy to toughen P3HB via catalytic carbonylative terpolymerization of enantiopure epoxides with different long alkyl side chains and configurations using a binary trimetallic CrIII complex/carbonyl cobalt catalyst system. The structure and stereochemistry of epoxides significantly impact the properties of the resultant PHA copolymers, which spans from viscous oil to tough/ductile and brittle plastics. This study represents a rather rare example of a method to toughen the extensively studied P3HB with epoxide and CO as raw materials.

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Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization

Cited by 28 publications

References 178 publications

Precision-engineering of subunit vaccine particles for prevention of infectious diseases

Precision-engineering of subunit vaccine particles for prevention of infectious diseases

Biomedical Applications of Polyhydroxyalkanoate in Tissue Engineering

Toughening Poly(3-hydroxybutyrate) by Using Catalytic Carbonylative Terpolymerization of Epoxides

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