Studies on Poliovirus Inhibitors in Sera of Domestic Animals

Kanamitsu, Masatsugu; Hashimoto, Nobuo; Urasawa, Shozo; Chiba, Shunzo

doi:10.7883/yoken1952.20.471

Cited by 7 publications

(2 citation statements)

References 33 publications

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“…It is known that virus neutralizing test in a cell-free system does not always give a true indication of the presence of poliovirus inhibitor in normal animal serum (Takemoto and Habel, 1959;Pagano et al, 1965;Thomssen et al, 1966;Kanamitsu et al, 1965Kanamitsu et al, , 1967. The present study was conducted to investigate the capability of normal serum inhibitors to combine with the virus, by using the precipitation test in agar gel.…”

Section: Discussionmentioning

confidence: 91%

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Studies on Poliovirus Inhibitors in Sera of Domestic Animals

Urasawa¹,

Urasawa²,

Chiba³

et al. 1968

JJMSB

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Section: Discussionmentioning

confidence: 91%

“…Serum: Most of samples of equine and bovine sera used in the present study were the same as those described in the previous paper (Kanamitsu et al, 1967). To obtain sera with high inhibitory activity, another groups of horses and cattle were bled .…”

Section: Methodsmentioning

confidence: 99%

Studies on Poliovirus Inhibitors in Sera of Domestic Animals

Urasawa¹,

Urasawa²,

Chiba³

et al. 1968

JJMSB

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Radioimmunoelectrophoretic identification of poliovirus inhibitors and their characteristic mode of action

Urasawa

Kanamitsu

1971

Archiv f Virusforschung

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SummaryPoliovirus inhibitors present in normal equine and bovine sera were studied by means of radioimmunoeleetrophoresis and column chromatography. The inhibitors in individual equine sera were identified as either one of the three immunoglobulins, yM, yA(T) and yG, or mixtures of them, while those in bovine sera were found to be either one of yM and yG globulins, or the mixture of the two.The inhibitors belonging to different classes of immnnoglobulin differed in their mode of action against the virus, yM inhibitors were characterized by distinct activities in the kinetic neutralization and the plaque reduction tests. In contrast, yA(T) inhibitors were highly active in the plaque-reduction and the precipitationin-agar-gel tests, although they were inactive in the kinetics of neutralization. There were two types of yG inhibitors. One did not neutralize the virus in the kinetic test, despite its distinct virus-precipitating activity. The other could neutralize but not precipitate the virus in vitro.Avidity of the inhibitors as revealed by dissociation of virus-inhibitor complexes at acidic pH's also differed among the immunoglobulin classes of the inhibitors. The complex formed with the NaIO4-treated yA(T) inhibitor was most stable and was not dissociated even at pH as low as 2.0.

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