Radioimmunoelectrophoretic identification of poliovirus inhibitors and their characteristic mode of action

Abstract

SummaryPoliovirus inhibitors present in normal equine and bovine sera were studied by means of radioimmunoeleetrophoresis and column chromatography. The inhibitors in individual equine sera were identified as either one of the three immunoglobulins, yM, yA(T) and yG, or mixtures of them, while those in bovine sera were found to be either one of yM and yG globulins, or the mixture of the two.The inhibitors belonging to different classes of immnnoglobulin differed in their mode of action against the virus, yM inhibitors were characterized by distinct activities in the kinetic neutralization and the plaque reduction tests. In contrast, yA(T) inhibitors were highly active in the plaque-reduction and the precipitationin-agar-gel tests, although they were inactive in the kinetics of neutralization. There were two types of yG inhibitors. One did not neutralize the virus in the kinetic test, despite its distinct virus-precipitating activity. The other could neutralize but not precipitate the virus in vitro.Avidity of the inhibitors as revealed by dissociation of virus-inhibitor complexes at acidic pH's also differed among the immunoglobulin classes of the inhibitors. The complex formed with the NaIO4-treated yA(T) inhibitor was most stable and was not dissociated even at pH as low as 2.0.

Electron Microscopic Examination of the Reactivity of Three Kinds of Neutralizing Antibodies with H Particles of Poliovirus

“…prep~ring t~e .abovementioned neutralizing antibodies and of the differences m their specificity are described in the previous paper (15). Neutralizing antibody titers were determined by plaque reduction assay as described previously (14).…”

ENTEROVIRUS TYPE 70-NEUTRALIZING IgM IN ANIMAL SERA

“…A similar sero-epidemiologic observations have been made by various investigators on the NT activity in various animal sera against polio viruses and other enteroviruses (Takemori et al, 1958;Ackerman and Dinka, 1965;Pagano, Gilden and Sedwick, 1965;Urasawa and Kanamitsu, 1971;Takemoto and Habal, 1959;Thomssen, Gerrit and Andreas, 1966;Bartell and Klein, 1955;Svehag, 1964) and recently against insect picornaviruses which have similar characteristics with animal enteroviruses (Longworth et al, 1973;Scotti and Longworth, 1980). One of the common features of these NT substances was that they were found to belong to IgM except in one study in which NT activity against poliovirus was found both in IgG and IgM fractions of horse and bovine sera (Urasawa et al, 1971).…”

“…One of the common features of these NT substances was that they were found to belong to IgM except in one study in which NT activity against poliovirus was found both in IgG and IgM fractions of horse and bovine sera (Urasawa et al, 1971). Most of the investigators have considered these NT substances likely to be antibody.…”

Monoclonal Antibodies Reactive with Different Sites of N and H Antigenic Particles of Poliovirus

“…The mixture was then processed for electron microscopy as described previously (Taniguchi et al, 1981). Neutralizing antibody titres were determined by 50~o plaque reduction assay as previously described (Urasawa et al, 1971).…”