Studies on NO releasing from PCU grafted with endogenous NO donors

Ye, Ya Qin; Feng, Yakai; Zhao, Hai

doi:10.1002/pat.1479

Cited by 14 publications

(7 citation statements)

References 29 publications

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“…The result showed that this film can catalyze NO release in PBS medium for about 2 d (Fig. 12), this result is similar to our previous studies on NO releasing from PCU film after being grafted with L‐CySNO 41. After 3 d, NO did not release any more.…”

Section: Resultssupporting

confidence: 89%

Polycarbonateurethane films containing complex of copper(II) catalyzed generation of nitric oxide

Zhao

Feng

Guo

2011

J of Applied Polymer Sci

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Nitric oxide (NO) is a well known potent antiplatelet agent, and its continuous release will effectively prevent the adhesion of platelets on artificial blood vessel walls. In this paper, polycarbonateurethane (PCU) with lipophilic Cu(II)-complex (Cu(II)-DTTCT) blending films were prepared and used as catalyst to generate NO from nitrite. The mechanical properties of PCU films blended with Cu(II)-DTTCT were characterized by tensile strength measurement. The tensile stress and Young's modulus of PCU films blending with Cu(II)-DTTCT increased, however, the elongation at break decreased compared with corresponding PCU films. The NO generation was investigated in vitro in the presence of NaNO 2 and ascorbic acid in PBS (pH ¼ 7.4) at 37 C. The flux of NO generation was quantitatively measured by Griess assay. NO flux and velocity increased with the increase of NaNO 2 concentration, the concentration of ascorbic acid in PBS and the amount of Cu(II) in the films. The loss of Cu(II) from blending film surfaces was found during the in vitro NO generation experiments, which resulted in the decrease of NO flux in the second run. The PCU film could catalyze continually generation of NO for two days, which will provide a promising approach that enable endogenous NO generation on the surface of the medical devices. The generation of biologically active level of NO at the blood/polymer interface can reduce the risk of thrombosis on the implants. Polycarbonateurethane films with NO generation function may be used as high thromboresistant blood contacting materials or coating.

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Section: Resultssupporting

confidence: 89%

Polycarbonateurethane films containing complex of copper(II) catalyzed generation of nitric oxide

Zhao

Feng

Guo

2011

J of Applied Polymer Sci

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“…artificial blood vessels, small-diameter vascular grafts) as they provide relatively beneficial thromboresistance, biocompatibility and mechanical properties similar to the polyether-based polyurethanes. [1][2][3][4] However, thrombus formation remained a critical risk factor. [5][6][7] An effective way to improve hemocompatibility of PCU is to optimize its material surface but not significantly change their intrinsic mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Plasma functionalization of polycarbonaturethane to improve endothelialization—Effect of shear stress as a critical factor for biocompatibility control

Lukas

Thomas²,

Gessner

et al. 2016

J Biomater Appl

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Medical devices made of polycarbonaturethane (PCU) combine excellent mechanical properties and little biological degradation, but restricted hemocompatibility. Modifications of PCU might reduce platelet adhesion and promote stable endothelialization. PCU was modified using gas plasma treatment, binding of hydrogels, and coupling of cellactive molecules (modified heparin, anti-thrombin III (ATIII), argatroban, fibronectin, laminin-nonapeptide, peptides with integrin-binding arginine-glycine-aspartic acid (RGD) motif). Biocompatibility was verified with static and dynamic cell culture techniques. Blinded analysis focused on improvement in endothelial cell (EC) adhesion/proliferation, antithrombogenicity, reproducible manufacturing process, and shear stress tolerance of ECs. EC adhesion and antithrombogenicity were achieved with 9/35 modifications. Additionally, 6/9 stimulated EC proliferation and 3/6 modification processes were highly reproducible for endothelialization. The latter modifications comprised immobilization of ATIII (A), polyethyleneglycole-diamine-hydrogel (E) and polyethylenimine-hydrogel connected with modified heparin (IH). Under sheer stress, only the IH modification improved EC adhesion within the graft. However, ECs did not arrange in flow direction and cell anchorage was restricted. Despite large variation in surface modification chemistry and improved EC adhesion under static culture conditions, additional introduction of shear stress foiled promising preliminary data. Therefore, biocompatibility testing required not only static tests but also usage of physiological conditions such as shear stress in the case of vascular grafts.

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“…Polycarbonateurethanes (PCUs) have been widely used as materials for medical applications in recent years as they provide compared to other synthetic materials relatively beneficial hemocompatibility and mechanical properties . However, thrombus formation could still occur when artificial organs and biomedical devices made of PCU were in contact with blood for extended time periods .…”

Section: Introductionmentioning

confidence: 99%

Functionalization of Polycarbonate Surfaces by Grafting PEG and Zwitterionic Polymers with a Multicomb Structure

Yang

Behl

et al. 2013

Macromolecular Bioscience

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The hemocompatibility of polycarbonateurethane (PCU) surfaces is improved by decoration with poly(poly(ethylene glycol) methacrylate) (poly(PEGMA)) and zwitterionic poly(3-((2-(methacryloyloxy)ethyl)dimethylammonio)propane-1-sulfonate) (poly(DMAPS)) blocks providing a novel multicomb structure obtained by application of surface-initiated atom transfer radical polymerization (s-ATRP) conditions. The PCU-poly(PEGMA-g-DMAPS) surface shows high hydrophilicity with a low contact angle of 20.6 ± 1.8°, while PCU-poly(PEGMA-b-DMAPS) surface exhibitsed a contact angle of 30.5 ± 2.6°. Furthermore, PCU-poly(PEGMA-g-DMAPS) surface shows very low platelet adsorption indicating that multicomb structure modified PCUs are preferred candidate materials for blood-contacting materials.

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Studies on NO releasing from PCU grafted with endogenous NO donors

Cited by 14 publications

References 29 publications

Polycarbonateurethane films containing complex of copper(II) catalyzed generation of nitric oxide

Polycarbonateurethane films containing complex of copper(II) catalyzed generation of nitric oxide

Plasma functionalization of polycarbonaturethane to improve endothelialization—Effect of shear stress as a critical factor for biocompatibility control

Functionalization of Polycarbonate Surfaces by Grafting PEG and Zwitterionic Polymers with a Multicomb Structure

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