Polycarbonateurethane films containing complex of copper(II) catalyzed generation of nitric oxide

Abstract

Nitric oxide (NO) is a well known potent antiplatelet agent, and its continuous release will effectively prevent the adhesion of platelets on artificial blood vessel walls. In this paper, polycarbonateurethane (PCU) with lipophilic Cu(II)-complex (Cu(II)-DTTCT) blending films were prepared and used as catalyst to generate NO from nitrite. The mechanical properties of PCU films blended with Cu(II)-DTTCT were characterized by tensile strength measurement. The tensile stress and Young's modulus of PCU films blending with Cu(II)-DTTCT increased, however, the elongation at break decreased compared with corresponding PCU films. The NO generation was investigated in vitro in the presence of NaNO 2 and ascorbic acid in PBS (pH ¼ 7.4) at 37 C. The flux of NO generation was quantitatively measured by Griess assay. NO flux and velocity increased with the increase of NaNO 2 concentration, the concentration of ascorbic acid in PBS and the amount of Cu(II) in the films. The loss of Cu(II) from blending film surfaces was found during the in vitro NO generation experiments, which resulted in the decrease of NO flux in the second run. The PCU film could catalyze continually generation of NO for two days, which will provide a promising approach that enable endogenous NO generation on the surface of the medical devices. The generation of biologically active level of NO at the blood/polymer interface can reduce the risk of thrombosis on the implants. Polycarbonateurethane films with NO generation function may be used as high thromboresistant blood contacting materials or coating.

Electrospun PCL/keratin/AuNPs mats with the catalytic generation of nitric oxide for potential of vascular tissue engineering

“…NO content was tested according to the published method with slight modification . In brief, the circle mats were put into bottle A containing 5 mL of working solution (65 μM S ‐nitrosoglutathione [GSNO], 100 μM GSH, and 500 μM EDTA in phosphate‐buffered solution).…”

“…In vitro catalytic NO generation NO content was tested according to the published method with slight modification. 26,27 In brief, the circle mats were put into bottle A containing 5 mL of working solution (65 μM S-nitrosoglutathione [GSNO], 100 μM GSH, and 500 μM EDTA in phosphate-buffered solution). The bottle A was connected with bottle B with plastic tube, so the generated NO from bottle A was poured into bottle B containing PBS solution by N 2 .…”

Recent Advances in High Performance Polymers—Tribological Aspects

“…For example, the expression for lubricated circumstances was theoretically formulated from the perceptions of crack progression, damage build-up, and traditional fracture mechanics, as reported by Lhymn et al [89]. Accordingly, the rate of wear is inversely proportionate to the product of the elastic modulus (E), hardness (H), and elongation at break (ε), as shown in Equation 7 [105,106]:…”

Synthesis of a polyurea from a glucose- or mannose-containingN-alkyl urea peptoid oligomer

“…Nitric oxide (NO) releasing polyurethanes have also been synthesized due to the strong antiplatelet adhesion abilities of NO to inhibit blood coagulation. [32][33][34] Related works have been published by Meyerhoff's group 32,33 and Guo's group. 34 Herein, we report the synthesis of heparin-mimicking sulfated-polyureas, using N-alkyl urea peptoid oligomers with glucose or mannose as pendant groups as the diamine monomer.…”

“…[32][33][34] Related works have been published by Meyerhoff's group 32,33 and Guo's group. 34 Herein, we report the synthesis of heparin-mimicking sulfated-polyureas, using N-alkyl urea peptoid oligomers with glucose or mannose as pendant groups as the diamine monomer. Polyureas are similar to polyurethanes and the two functional groups (urethane and urea) can easily be incorporated into single polymer chains through using appropriate amounts of diamine versus diol comonomers.…”