Studies on Morphological Changes and Histamine Release Induced by Bee Venom, n‐Decylamine and Hypotonic Solutions in Rat Peritoneal Mast Cells

Abstract

BLOOM, G. D. and 0. HAEGERMARK. Studies on morphological changes and histamine release induced b y bee venom, n-decylamine and hypotonic solutions in rat peritoneal mast cells.

EFFECTS OF n-DECYLAMINE AND TOLUIDINE BLUE ON THE ELECTRIC CAPACITANCE OF ISOLATED RAT MAST CELLS

“…In association with this, Cole (11) reported the membrane capacity of hipponoe eggs decreased as a linear function of the surface area when eggs were swollen in diluted sea water, interpreting this as being a result of a constitutional change in the membrane. The finding in the present experiment coincides with electron microscopic observations (18) that n-decylamine disrupts not only the cytoplasmic but also the perigranular membranes so as to give a fragmented appearance in each, and most of the granules were swollen and grossly altered exhibiting a coarse and low electron density. Impairing the membrane necessitates an intrusion of extracellular fluid into the cytoplasm and this results in subsequent increase of the cell volume.…”

Secretion of the Content of Granules and Its Relationship to the Acid Phosphatase Reaction in Regenerating Rat Peritoneal Mast Cells

“…The intracellular structures of mast cells such as nucleus, mitochondria, Golgi apparatus, were similar to those that have been described by many authors (1,2,3,6,7,8).…”

“…Infrequently observed reticular granules with positive AcPase reaction in a few mast cells of the control animals are the same ones which were described by many authors as "altered" granules in a few mast cells of control animals (1,2,3,6,7,8). The fine to coarse reticular granules in our regenerating mast cells are very similar (a) No reaction products in the granules, Only minimum reaction products on the surface of granules.…”

“…to the "swollen" granules in various experimental conditions, such as histamine liberation or degranulation, of other workers (l, 2,3,6,7,8). Minor differences between our reticular granules and swollen granules of other workers might be due to differences of the fixative and staining methods.…”

Compound versus multigranular exocytosis in peritoneal mast cells.

“…Compound exocytosis seems to be the major mechanism of secretion in mast cells stimulated either with compound 48/80 (Rohlich et al, 1971), bee venom (Bloom and Haegermark, 1967), or with antigens in sensitized cells (Anderson et al, 1973). Also, compound exocytosis seems to occur in human platelets (Morgenstern et al, 1987) and neutrophils (Chandler et al, 1983;Chandler and Kazilek, 1986).…”