1969
DOI: 10.1016/s0021-9258(18)91738-2
|View full text |Cite
|
Sign up to set email alerts
|

Studies on Milk Xanthine Oxidase

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

3
55
0

Year Published

1969
1969
2011
2011

Publication Types

Select...
6
3

Relationship

0
9

Authors

Journals

citations
Cited by 368 publications
(58 citation statements)
references
References 31 publications
3
55
0
Order By: Relevance
“…There has been a tendency to assume (cf. Massey, Brumby, Komai & Palmer, 1969) that all of these constituents would not be likely to function as a single catalytic unit in the molecule and that there must be two presumably equivalent active centres (Bray, 1963). We now present evidence based on FAD loss (Bray, Chisholm, Hart, Meriwether & Watts, 1966) during inactivation of the reduced enzyme by iodoacetamide (Bray & Watts, 1965) indicating that xanthine oxidase operates, during aerobic xanthine oxidation at least, with a single active centre.The interpretation of earlier quantitative studies on reaction ofxanthine oxidase with iodoacetamide, by measuring iodide liberation (Bray & Watts, 1965) and following FAD loss (A. J. Chisholm, A. M. Scott & R. C. Bray, unpublished work), was complicated by the use as starting material of preparations with non-stoicheiometric FAD/Mo ratios.…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…There has been a tendency to assume (cf. Massey, Brumby, Komai & Palmer, 1969) that all of these constituents would not be likely to function as a single catalytic unit in the molecule and that there must be two presumably equivalent active centres (Bray, 1963). We now present evidence based on FAD loss (Bray, Chisholm, Hart, Meriwether & Watts, 1966) during inactivation of the reduced enzyme by iodoacetamide (Bray & Watts, 1965) indicating that xanthine oxidase operates, during aerobic xanthine oxidation at least, with a single active centre.The interpretation of earlier quantitative studies on reaction ofxanthine oxidase with iodoacetamide, by measuring iodide liberation (Bray & Watts, 1965) and following FAD loss (A. J. Chisholm, A. M. Scott & R. C. Bray, unpublished work), was complicated by the use as starting material of preparations with non-stoicheiometric FAD/Mo ratios.…”
mentioning
confidence: 99%
“…There has been a tendency to assume (cf. Massey, Brumby, Komai & Palmer, 1969) that all of these constituents would not be likely to function as a single catalytic unit in the molecule and that there must be two presumably equivalent active centres (Bray, 1963). We now present evidence based on FAD loss (Bray, Chisholm, Hart, Meriwether & Watts, 1966) during inactivation of the reduced enzyme by iodoacetamide (Bray & Watts, 1965) indicating that xanthine oxidase operates, during aerobic xanthine oxidation at least, with a single active centre.…”
mentioning
confidence: 99%
“…), stained with saturated ethanolic uranylacetate and lead citrate, and examined in a Philips EM 300. Protein concentrations were obtained by the method of Lowry et al ( 9), 5'-nueleotidase, thiamine pyrophosphatase, glucose-6-phosphatase, and succinic dehydrogenase ( 14), xanthine oxidase (12), and galactosyltransferase (18,21) as described in the literature. UDPase assays were performed as for thiamine pyrophosphatase, using 2 mM UDP.…”
Section: Methodsmentioning
confidence: 99%
“…XO was isolated and purified by the method of Massey et a1. 6 The identity and purity of the enzyme was ascertained by optical spectra, AFR (=120; activity to flavin ratio) and PFR (=5.8; protein to flavin ratio) values. Analytical grade PdC12 and NaH2P04 were used.…”
Section: Chemical Physics Group Tata Institute Of Fundamental Researc...mentioning
confidence: 99%