Studies on micropropagation of gerbera (Gerbera jamesonii Bolus)

Bhargava, Bhavya; Dilta, BS; Gupta, Y. C.; Dhiman, SR; Modgil, Manju

doi:10.15373/2249555x/nov2013/4

Cited by 7 publications

(5 citation statements)

References 1 publication

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“…Many researchers have reported successful shoot regeneration from capitulum explant (Hussein et al 2008;Bhatia et al 2011). Similar to the results obtained in this study, Warar et al (2008) and Bhargava et al (2013) have also reported efficiency of BA and IAA in in vitro shoot induction from capitulum explant. The dose of radiation at which 50% survival or mortality is obtained is called lethal dose LD 50 .…”

Section: Discussionsupporting

confidence: 87%

Induction of powdery mildew resistance in gerbera (Gerbera jamesonii) through gamma irradiation

Ghani¹,

Sharma

2018

Physiol Mol Biol Plants

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In this study, the effect of gamma irradiation in inducing resistance/tolerance towards powdery mildew disease was investigated in Gerbera jamesonii cv. 'Harley'. In vitro shoot cultures were established through capitulum explants on Murashige and Skoog medium supplemented with 22.2 lM 6-benzyladenine (BA) and 2.53 lM indole acetic acid (IAA), followed by gamma irradiation of regenerated shoots (3-5 cm). Activity of four antioxidant enzymes i.e. superoxide dismutase, ascorbate peroxidase, catalase and glutathione reductase increased significantly as compared to the control and reached to highest level at the most stringent doses of mutagen. Ninety randomly selected irradiated plants (6 months old) and 100 control plants were inoculated with fungal conidial suspension, to screen for tolerance/resistance against powdery mildew. The severity of the disease was recorded on 0-4 scale with '0' indicating highly resistant; '1' indicating resistant; '2' indicating medium resistance; '3' indicating susceptible and '4' indicating highly susceptible. Three plants (3.33%) irradiated with 5 Gy were found to be tolerant to powdery mildew as these plants showed slight and delayed development of fungal colonies on the leaves. The random amplified polymorphic DNA characterization showed that the irradiated plants had DNA patterns that were different from the control and mother plants.

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Section: Discussionsupporting

confidence: 87%

Induction of powdery mildew resistance in gerbera (Gerbera jamesonii) through gamma irradiation

Ghani¹,

Sharma

2018

Physiol Mol Biol Plants

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“…In the commercial production of gerberas, micropropagation enables the large-scale production of plants in a limited space, as well as the obtaining of disease-and pest-free uniform plants (Bhargava et al, 2013). This technique also ensures precision in production schedules and product quality, regarding plant homogeneity and vigor.…”

Section: Introductionmentioning

confidence: 99%

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Pais¹,

Silva²,

Souza³

et al. 2016

Afr. J. Biotechnol.

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Micropropagation requires controlling contamination that might compromise the success of the process. Thermal sterilization is traditionally used; however, costs deriving from equipment acquisition and maintenance render this technique costly. With the purpose of finding an alternative to thermal sterilization, this research aimed at assessing the efficiency and ideal concentration of sodium hypochlorite for sterilization of culture media and glassware used during rooting of micropropagated Gerbera hybrida cv. Essandre. Two experiments were carried out. In the first one, treatments consisted of control I (no sterilization), control II (thermal sterilization), and total active chlorine concentrations of 0.0005, 0.001, 0.002 and 0.003%. In the second experiment, based on the results observed in the first experiment, treatments consisted of control I (thermal sterilization) and II (chemical sterilization), and total active chlorine concentrations of 0.002, 0.0025 and 0.003%. Plant behavior was assessed based on the length of aerial part and roots, number of roots, and dry biomass of plants. Results showed that the addition of an active chlorine concentration of 0.003% to culture media provided total control of contaminants, and there were no significant differences regarding the variables analyzed between plants obtained with thermal sterilization and with sodium hypochlorite sterilization. Thus, chemical sterilization can be used as a replacement for thermal sterilization of nutrition media for rooting of gerbera in vitro.

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“…This problem was also reported by Mishra and Datta (2001). Bhargava et al (2013) also reported significant improvement in shoot proliferation with the use of growth hormones and linear increase in number of shoots with increased concentration of cytokinins and their combinations. They also reported that MS medium supplemented with 4mgl -1 BAP and 2mgl -1 kinetin was found optimum for proliferation of quality shoots in gerbera.…”

Section: Resultsmentioning

confidence: 91%

Effect of Ga3 on in Vitro Propagation of White Marigold

ijrbat¹

2015

IJRBAT

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Experiment was carried out during 2014-15 at Botany Section, College of Agriculture, Nagpur to identify an in vitro protocol for the propagation of white marigold and also to study the effect of gibrellic acid on in vitro propagation. The explants axillary buds were used for culturing in the MS basal fortified with growth regulator like BAP and IAA in different concentrations along with GA3 (14.43µM) and without GA3. GA3 played significant role in the induction of shoot buds as well as suppressing callus formation. The axillary bud explants responded well for all the traits studied. The treatment T16 (MS + BAP 4.44µm + IAA 2.84 µm + GA3 14.43 µm) and T11 (MS + BAP 4.44µm + GA3 14.43 µm) exhibited good performance for response to shoot initiation (%), days to shoot initiation, number of shoots culture -1 and number of shoots elongated culture -1 . Average performance recorded for response of shoot initiation(%) was 68.61%, days to shoot initiation was 14.62 days, number of shoots culture -1 was 5.01, number of shoots elongated culture -1 was 3.49, response of root initiation (%) was 57.58 and days to root initiation was 7.81. The shoot propagated from axillary bud were found to root well in treatment T5 (MS+ NAA 0.27µm) and T6 (MS + NAA 0. 54µm). Thus, in vitro propagation of white marigold can be done successfully by inoculating axillary bud explants in T16 (MS + BAP 4.44µm + IAA 2.84 µm + GA3 14.43 µm) and T11 (MS + BAP 4.44µm + GA3 14.43 µm) for shoot induction and proliferation followed by transferring the shoots to T5 (MS+ NAA 0.27µm) for rooting.

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Studies on micropropagation of gerbera (Gerbera jamesonii Bolus)

Cited by 7 publications

References 1 publication

Induction of powdery mildew resistance in gerbera (Gerbera jamesonii) through gamma irradiation

Induction of powdery mildew resistance in gerbera (Gerbera jamesonii) through gamma irradiation

Sodium hypochlorite sterilization of culture medium in micropropagation of Gerbera hybrida cv. Essandre

Effect of Ga3 on in Vitro Propagation of White Marigold

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