Studies on lipid peroxidation in the rat brain

Cini, M.; Fariello, Ruggero G.; Bianchetti, Angelo; Moretti, Antonio

doi:10.1007/bf00971576

Cited by 126 publications

(75 citation statements)

References 35 publications

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“…MDA is a major reactive aldehyde that is formed during the final stages of lipid peroxidation of biological membrane polyunsaturated fatty acid (Cini et al, 1994). Our results demonstrated that the administration of H 2 O 2 caused an increase in the MDA concentration of the human dermal fibroblasts, while the application of Oatp decreased the MDA level, suggesting that Oatp was effective in scavenging free radicals and suppressing the production of MDA.…”

Section: Oatp Intervention In H 2 O 2 -Induced Fibroblast Damage Withmentioning

confidence: 53%

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Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide

Feng

Ma²,

Yao

et al. 2013

J. Zhejiang Univ. Sci. B

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Abstract:Oat contains different components that possess antioxidant properties; no study to date has addressed the antioxidant effect of the extract of oat bran on the cellular level. Therefore, the present study focuses on the investigation of the protective effect of oat bran extract by enzymatic hydrolysates on human dermal fibroblast injury induced by hydrogen peroxide (H 2 O 2 ). Kjeldahl determination, phenol-sulfuric acid method, and high-performance liquid chromatography (HPLC) analysis indicated that the enzymatic products of oat bran contain a protein amount of 71.93%, of which 97.43% are peptides with a molecular range from 438.56 to 1 301.01 Da. Assays for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity indicate that oat peptide-rich extract has a direct and concentration-dependent antioxidant activity. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay and the TdT-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay for apoptosis showed that administration of H 2 O 2 in human dermal fibroblasts caused cell damage and apoptosis. Pre-incubation of human dermal fibroblasts with the Oatp for 24 h markedly inhibited human dermal fibroblast injury induced by H 2 O 2 , but application oat peptides with H 2 O 2 at same time did not. Pre-treatment of human dermal fibroblasts with Oatp significantly reversed the H 2 O 2 -induced decrease of superoxide dismutase (SOD) and the inhibition of malondialdehyde (MDA). The results demonstrate that oat peptides possess antioxidant activity and are effective against H 2 O 2 -induced human dermal fibroblast injury by the enhanced activity of SOD and decrease in MDA level. Our results suggest that oat bran will have the potential to be further explored as an antioxidant functional food in the prevention of aging-related skin injury.

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Section: Oatp Intervention In H 2 O 2 -Induced Fibroblast Damage Withmentioning

confidence: 53%

“…MDA is a byproduct of lipid peroxidation and is widely used as a biomarker of oxidative stress (Cini et al, 1994). However, antioxidant enzymes, such as SOD and glutathione (GSH)-Px, are considered to effectively augment cells' antioxidant defenses (Luo and Xia, 2006).…”

Section: Oatp Intervention In H 2 O 2 -Induced Fibroblast Damage Withmentioning

confidence: 99%

Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide

Feng

Ma²,

Yao

et al. 2013

J. Zhejiang Univ. Sci. B

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“…When the ascorbate system was used, liver microsomes were heat-inactivated before the addition of the incubation mixture. Brain microsomal LP was determined according to Cini et al, 1994, using microsomes instead of brain homogenates. Microsomes were incubated at 37°C for 60 min without (spontaneous) and with the addition of a free-radical generator system (100 µM FeCl 2 ).…”

Section: Assay Of Lp In Liver and Brain Microsomesmentioning

confidence: 99%

In Vitro effect of D‐004, a lipid extract of the ground fruits of the cuban royal palm (Roystonea regia), on rat microsomal lipid peroxidation

Menéndez

Más

Pérez

et al. 2006

Phytotherapy Research

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“…MDA is a by-product of lipid peroxidation induced by excessive ROS and is widely used as a biomarker of oxidative stress [24] . Antioxidant enzymes, such as SOD and GSH-Px, however, are thought to be effective for the augmentation of antioxidant defenses in endothelial cells [25] .…”

Section: Discussionmentioning

confidence: 99%

Protective effects of prostaglandin E1 on human umbilical vein endothelial cell injury induced by hydrogen peroxide

Fang

Zhou

2010

Acta Pharmacol Sin

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Aim: To investigate the protective effects of prostaglandin E 1 (PGE 1 ) against H 2 O 2 -induced oxidative damage on human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were pretreated with PGE 1 (0.25, 0.50, and 1.00 µmol/L) for 24 h and exposed to H 2 O 2 (200 µmol/L) for 12 h, and cell viability was measured by the MTT assay. LDH, NO, SOD, GSH-Px, MDA, ROS, and apoptotic percentage were determined. eNOS expression was measured by Western blotting and real-time PCR. Results: PGE 1 (0.25−1.00 µmol/L) was able to markedly restore the viability of HUVECs under oxidative stress, and scavenged intracellular reactive oxygen species induced by H 2 O 2 . PGE 1 also suppressed the production of lipid peroxides, such as MDA, restored the activities of endogenous antioxidants including SOD and GSH-Px, and inhibited cell apoptosis. In addition, PGE 1 significantly increased NO content, eNOS protein, and mRNA expression. Conclusion: PGE 1 effectively protected endothelial cells against oxidative stress induced by H 2 O 2 , an activity that might depend on the up-regulation of NO expression.

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Studies on lipid peroxidation in the rat brain

Cited by 126 publications

References 35 publications

Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide

Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide

In Vitro effect of D‐004, a lipid extract of the ground fruits of the cuban royal palm (Roystonea regia), on rat microsomal lipid peroxidation

Protective effects of prostaglandin E1 on human umbilical vein endothelial cell injury induced by hydrogen peroxide

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