Protective effects of prostaglandin E1 on human umbilical vein endothelial cell injury induced by hydrogen peroxide

Abstract

Aim: To investigate the protective effects of prostaglandin E 1 (PGE 1 ) against H 2 O 2 -induced oxidative damage on human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were pretreated with PGE 1 (0.25, 0.50, and 1.00 µmol/L) for 24 h and exposed to H 2 O 2 (200 µmol/L) for 12 h, and cell viability was measured by the MTT assay. LDH, NO, SOD, GSH-Px, MDA, ROS, and apoptotic percentage were determined. eNOS expression was measured by Western blotting and real-time PCR. Results: PGE 1 (0.25−1.00 µmol/L) was able to markedly restore the viability of HUVECs under oxidative stress, and scavenged intracellular reactive oxygen species induced by H 2 O 2 . PGE 1 also suppressed the production of lipid peroxides, such as MDA, restored the activities of endogenous antioxidants including SOD and GSH-Px, and inhibited cell apoptosis. In addition, PGE 1 significantly increased NO content, eNOS protein, and mRNA expression. Conclusion: PGE 1 effectively protected endothelial cells against oxidative stress induced by H 2 O 2 , an activity that might depend on the up-regulation of NO expression.

The protective role of 5-HMF against hypoxic injury

“…The number of blue-stained dead cells and non-stained living cells were then counted with a hemacytometer within 3 min. The cell mortality rate (%) was calculated as the number of dead cells divided by the number of total cells, multiplied by 100 (Fang et al 2010;Valadares et al 2009;Wang et al 2009a, b).…”

Real-Time Monitoring of Nitric Oxide Dynamics in the Myocardium: Biomedical Application of Nitric Oxide Sensor

“…Prostaglandin E1 (Alprostadil ® , PGE1), which is an important member of the prostaglandin (PG) family, is a product of arachidonic acid metabolism by cyclooxygenase [66,67]. Similar to NO, PGE1 has cardioprotective effects during IR [67,68], as well as vasodilator effects on the systemic and pulmonary circulation [69].…”

“…Similar to NO, PGE1 has cardioprotective effects during IR [67,68], as well as vasodilator effects on the systemic and pulmonary circulation [69]. Fang et al reported that pretreatment of human umbilical vein endothelial cells with PGE1 significantly protected those cells from H 2 O 2 -induced cell death [66]. And this effect might depend, at least in part, on the upregulation of NO expression [66].…”

“…Fang et al reported that pretreatment of human umbilical vein endothelial cells with PGE1 significantly protected those cells from H 2 O 2 -induced cell death [66]. And this effect might depend, at least in part, on the upregulation of NO expression [66]. On the other hand, Huk et al reported that PGE1 prevents the excessive generation of NO, superoxide, and ONOO − which trigger a cascade of events leading to IR injury [70].…”

Efecto del alprostadil en los cultivos de músculo esquelético isquémico. Estudio proteómico comparativo frente a músculo sano

“…Este medio está constituido por la formulación basal estándar recogida en publicaciones previas (132) De cada grupo de muestras (I y S) se realizaron 2 cultivos, uno basal, sin fármaco, y otro con alprostadil, a una concentración de 5ng/ml. La concentración se basó en estudios similares realizados en células endoteliales (133,134) El medio se cambió cada 12 horas. Por tanto, de cada paciente se obtuvieron 4 cultivos (grupo I basal, grupo I con alprostadil, grupo S basal, grupo S con alprostadil).…”