Studies on Japanese B encephalitis virus III. Propagation and assay of Japanese B encephalitis virus in a stable line of porcine kidney cells

Inoue, Yumi; Ogura, R

doi:10.1016/0042-6822(62)90299-4

Cited by 32 publications

(9 citation statements)

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“…Culture fluids were tested for haemagglutinin using guinea pig, chicken and human erythrocytes. Isolates selected for further study were adapted to grow in porcine kidney stable (PS) line cells (Inoue and Ogura 1962). The particular cells used were apparently free of adventitious swine fever virus infection, since inoculated pigs developed no serological response to swine fever virus.…”

Section: Virus Isolation and Characterisationmentioning

confidence: 99%

The Characterisation and Pathogenicity of Porcine Enteroviruses Isolated in Victoria

Forman

Pass

Connaughton³

1982

Aust Veterinary J

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SUMMARY Approximately 23 viruses were isolated from healthy pigs, pigs with encephalitis, and in cases of reproductive failure. Five viruses were identified as enteroviruses and a total of 10 isolates were shown to cross‐react serologically to varying degrees. Twenty viruses were neutralised by a reference antiserum of serotype 8 porcine enterovirus. Intracerebral inoculation of colostrum‐deprived piglets with 2 of the characterised viruses caused lesions of encephalomyelitis which were not induced by oral infection. Intrafoetal inoculation of 2 sows with one characterised faecal isolate caused foetal death and abortion, but no adverse effects followed oral dosage.

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Section: Virus Isolation and Characterisationmentioning

confidence: 99%

The Characterisation and Pathogenicity of Porcine Enteroviruses Isolated in Victoria

Forman

Pass

Connaughton³

1982

Aust Veterinary J

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“…Annandale, New South Wales 496 mosphere of 7.5% C02. A suspension of PS cells (Inoue and Ogura 1962) was added to each well, being 0.05 ml at 5X105 cells/ml. The plates were sealed and incubated for 4 days at 37°C.…”

Section: Micro Biologymentioning

confidence: 99%

Atypical Porcine Enterovirus Encephalomyelitis: Possible Interraction Between Enteroviruses and Arsenicals

Forman

Connaughton

Gillick³

et al. 1979

Aust Veterinary J

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Porcine enteroviruses were isolated from weaner pigs that had nervous signs and mild non-suppurative meningoencephalomyelitis and ganglioneuritis. The clinical signs and lesions were not typical of enterovirus infection and it is believed that an organic arsenical present in feed enhanced pathogenicity of enteroviruses. Severe non-suppurative polioencephalomyelitis and ganglioneuritis were produced in gnotobiotic pigs by oral inoculation of the viruses.

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“…Inactivation of a JEV strain, Ja0Ar-121-71-C1, by heating at 45 C or 56 C. Table 4. Neutralization of a JEV strain, Ja0Ar-121-71-C1, by anti-viral sera 7 anti-JEV serum, fresh rabbit serum was added to the reaction system at 1:4 as a neutralization-potentiating factor. The results are presented in Fig.…”

Section: Mgc Formation In Monolayers Of Various Cellsmentioning

confidence: 99%

“…Japanese encephalitis virus ( JEV), a member of group B arboviruses, multiplies in arthropod, avian and mammalian cells, such as Singh's Aedes albopictus, chick embryo, hamster kidney, HeLa, BHK-21 or PS cells, showing either clear cytopathic effects (CPE) or none [2,6,7,9]. It has been reported that a number of enveloped viruses in the paramyxovirus, herpes virus and poxvirus groups produce multinucleated giant cells (MGC) in monolayers of certain cells.…”

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confidence: 99%

Multinucleated Giant Cell Formation in BHK‐21‐528 Cell Monolayers Infected with Japanese Encephalitis Viruses1

Ueba

Kimura

Kimoto

1976

Japanese Journal of Microbiology

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Formation of prominent multinucleated giant cells (MGC) was observed in monolayers of a clonal line of BHK‐21 cells (BHK‐21–528) when infected with Japanese encephalitis virus (JEV). MGC were first observed 3 to 4 days after infection and cytopathic changes proceeded thereafter. Formation of MGC is a typical cytopathic change in this clonal cell line. Virus titer in 50% tissue culture infective dose (TCID50) equaled that in 50% MGC‐forming dose. Virus titer in TCID50 was approximate to plaque‐forming units (PFU) in the same host cells. An ability of JEV to form MGC was maintained at least for six serial passages in BHK‐21–528. It was inactivated by heating at 56 C for 3 min. All JEV strains, except an attenuated live vaccine strain, induced formation of MGC in BHK‐21–528 cells. Red blood cells of several animal species were not adsorbed to MGC induced by JEV. The MGC‐forming ability of JEV was specifically neutralized by anti‐JEV serum. By fluorescence antibody technique, the MGC were specifically stained by anti‐JEV antibody conjugated with fluorescein isothiocyanate. Immunization of animals with lysates of the MGC resulted in production of antibodies against JEV, but no antibody against other viruses which have been reported to induce MGC formation. From these evidences, it was concluded that JEV induced formation of MGC in BHK‐21–528.

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Studies on Japanese B encephalitis virus III. Propagation and assay of Japanese B encephalitis virus in a stable line of porcine kidney cells

Cited by 32 publications

References 1 publication

The Characterisation and Pathogenicity of Porcine Enteroviruses Isolated in Victoria

The Characterisation and Pathogenicity of Porcine Enteroviruses Isolated in Victoria

Atypical Porcine Enterovirus Encephalomyelitis: Possible Interraction Between Enteroviruses and Arsenicals

Multinucleated Giant Cell Formation in BHK‐21‐528 Cell Monolayers Infected with Japanese Encephalitis Viruses1

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