Studies on In Vitro Transformation by DNA and DNA Fragments of Human Adenoviruses and Simian Virus 40

Graham, Frank L.; Abrahams, P. J.; Mulder, Carel; Heijneker, H. L.; Warnaar, S. O.; Vries, F. A. J. de; Fiers, Walter; Eb, A. J. Van Der

doi:10.1101/sqb.1974.039.01.077

Cited by 290 publications

(140 citation statements)

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“…SV40 DNA was also used to infect subconfluent monolayers as described by Graham et al (1974). For each infection, 10 ,tg of SV40 DNA was used and the cells plated out at 5 x 104 cells/5-cm dish as for the virusinfected cells.…”

mentioning

confidence: 99%

Chromosome complement and SV40 transformation of cells from patients susceptible to malignant disease

Webb¹,

Harding²

1977

Br J Cancer

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A comparative study has been made of fibroblasts obtained from patients with differing susceptibilities to malignant disease, both with respect to their chromosome complements and their transformation with SV40 virus. Fibroblasts from 2 Bloom's syndrome patients were found not to have raised SV40 transformation rates and no correlation was found between chromosome abnormality per se and transformation. Of 2 cell types with greatly increased rates, one was derived from a neurofibromatosis patient and the other from an A-T heterozygote. When SV40 DNA was employed as the transforming agent for the latter, the transformation rate was no longer raised. Images Fig. 1 Fig. 2

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mentioning

confidence: 99%

Chromosome complement and SV40 transformation of cells from patients susceptible to malignant disease

Webb¹,

Harding²

1977

Br J Cancer

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“…Purified DNA from human lymphoblastoid cells is taken up by human skin fibroblasts in culture and retained in a relatively undegraded form for over 20h [21]. T7 phage DNA however undergoes extensive degradation after entry into the fibroblasts [23]. On the other hand in cell fusion experiments in which mouse-human hybrid lines are produced some human chromosomes tend to be eliminated but only after several cell generations.…”

Section: Alsomentioning

confidence: 98%

“…Growing cells will be likely to modify whereas nongrowing cells appear to be more equipped to cleave. Successful use of naked herpes virus DNA [22] or adenovirus DNA [23] as infectious agents appears to have involved the use of growing monolayer cell cultures, however there have been no definitive experiments to answer this problem. Clearly this will be of relevance in any recombinant DNA techniques involving mammalian cells.…”

Section: Alsomentioning

confidence: 99%

Mammalian DNA methylation and a nuclear S‐adenosyl L‐methionine‐dependent nuclease activity

Cato¹,

Burdon²

1979

FEBS Letters

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“…The 293 cell line, obtained from F. Graham (Graham et al, 1974, was grown in Dulbecco's medium supplemented with 10~ foetal calf serum. Human adenovirus type 2 wild-type (H2WT) was originally obtained from J. Williams and serotype 3 from R. Marusyk.…”

Section: Ceils and Virusesmentioning

confidence: 99%

An Adenovirus Cytocidal Function Related to the Control of a Cellular pH 4 Endonuclease Activity

D'Halluin

Delsert

Milleville

et al. 1985

Journal of General Virology

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SUMMARYAn adenovirus (Ad) interserotypic recombinant (H2cytl41) between temperaturesensitive mutant H2tsl 11 of Ad2 and deletion mutant H5d1313 of Ad5 was isolated and characterized. It was phenotypically ts +, dl ÷, hr + and formed large plaques (or cytocidal: cyt). It contained the right 89 % of Ad5 DNA and the leftmost 11% of Ad2 DNA. Genetic recombination data suggested the cytocidal mutation lay in the transforming region E1B, confirming sequence analysis. The cytocidal effect resulted in part from the breakdown of cellular DNA. Host cell and virus DNA breakdown induced by H2cytl41 appeared cell-dependent: it occurred in HeLa, KB or BHK-21 cells, but not in CV1 or 293 cells. In human cells the cyt effect was recessive and adenovirus DNA degradation was prevented by co-infection with adenovirus wildtype (H2WT), other adenovirus serotypes or simian virus 40 (SV40). In simian cells, H2cytl41 did not inhibit SV40 DNA replication, unlike H2WT. The amount of H2cytl41 DNA integrated in human cell DNA at early stages of the lytic cycle was found to be significantly lower than for H2WT. Novobiocin inhibited viral DNA breakdown in human cells. Cellular DNA extracted from H2cytl41-infected cells exhibited a repeat band pattern in gel electrophoresis reminiscent of the nuclease digestion pattern of chromatin, with monosome-size fragments as the digestion limit. The H2cyt141-induced nucleolytic effect would therefore occur in the linker regions of cell DNA and might result from the observed stimulation (by a factor of greater than 100) of an acidic (optimum pH 4.0) endonuclease activity. The nucleolytic effect also appeared to be recessive in vitro and absent in mixed samples containing extracts from H2cyt141-infected cells plus extracts from H2WT-or mock-infected cells. The virus gene product responsible for the enhancement of the acidic endonuclease was found to function stoichiometrically and not catalytically. The cytocidal and nucleolytic effects of the viral E1B region 19K protein may be mediated by a cellular inhibitor of acidic endonuclease.

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Studies on In Vitro Transformation by DNA and DNA Fragments of Human Adenoviruses and Simian Virus 40

Cited by 290 publications

References 0 publications

Chromosome complement and SV40 transformation of cells from patients susceptible to malignant disease

Chromosome complement and SV40 transformation of cells from patients susceptible to malignant disease

Mammalian DNA methylation and a nuclear S‐adenosyl L‐methionine‐dependent nuclease activity

An Adenovirus Cytocidal Function Related to the Control of a Cellular pH 4 Endonuclease Activity

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