Studies on in vitro Antibacterial, Antifungal Property and Antioxidant Potency of Murraya paniculata

Sundaram, M.; SIVAKUMAR, .; Karthikeyan, .; Bhuvaneshwari,; Aishwarya, .; Thirumalai,; Pennarasi,

doi:10.3923/pjn.2011.925.929

Cited by 14 publications

(12 citation statements)

References 15 publications

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“…Current literature describes M. paniculata as having antihyperglycemic (Gautam et al. 2012a ), antifungal (Sundaram 2011 ), antibacterial (Rodanant et al. 2015 ) analgesic (Fazal-ur-Rehman et al.…”

Section: Introductionmentioning

confidence: 99%

Effects of β-caryophyllene and Murraya paniculata essential oil in the murine hepatoma cells and in the bacteria and fungi 24-h time–kill curve studies

Neta

Vittorazzi

Guimarães

et al. 2016

Pharmaceutical Biology

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Context: Orange Jessamine [Murraya paniculata L. (Rutaceae)] has been used worldwide in folk medicine as an anti-inflammatory, antibiotic and analgesic.Objective: The objective of this study is to investigate the in vitro antioxidant, cytotoxic, antibacterial and antifungal activity and the time-kill curve studies of orange jessamine essential oil and β-caryophyllene, as well as the chemical composition of the essential oil.Material and methods: The cytotoxic activity of M. paniculata and β-caryophyllene (7.8–500 μg/mL) was evaluated using the MTT assay on normal fibroblasts and hepatoma cells. The minimal inhibitory concentration and time–kill curves (24 h) were evaluated against those of Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, Enterococcus faecallis, Aspergillus (niger, fumigates and parasiticum) and F. solani by the broth microdilution method. The antioxidant activity was measured by the DPPH and ABTS assays. Chemical composition was evaluated by GC/MS analyses.Results: GC/MS analyses identified 13 compounds, with β-caryophyllene as the major compound. The oil exhibited moderate antibacterial activity (MIC <1.0 mg/mL) and strong antifungal activity. Time–kill curve studies showed that either the essential oil or β-caryophyllene presented rapid bacterial killing (4 h for S. aureus) and fungicidal effect (2-4 h for F. solani); however, both displayed weak free radical scavenger capacity. The cytotoxic activity exhibited a prominent selective effect against hepatoma cancer cells (IC50 value =63.7 μg/mL) compared with normal fibroblasts (IC50 value =195.0 μg/mL), whereas the β-caryophyllene showed low cytotoxicity.Discussion and conclusion: The experimental data suggest that the activities of M. paniculata essential oil are due to the synergistic action among its components.

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Section: Introductionmentioning

confidence: 99%

Effects of β-caryophyllene and Murraya paniculata essential oil in the murine hepatoma cells and in the bacteria and fungi 24-h time–kill curve studies

Neta

Vittorazzi

Guimarães

et al. 2016

Pharmaceutical Biology

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“…Sundaram (2011) showed that the ethanolic extract of leaves had not antimicrobial activity against E. coli. Gautam et al (2012b) correlated high content of phenolics and flavonoids in M. paniculata leaf extract with antibacterial properties against human pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…On phytochemical investigation, researchers have reported that Murraya leaves contain flavonoids (Zhang et al, 2011(Zhang et al, , 2012, cinnamates, and coumarins (Aziz et al, 2010;Ito et al, 2005;Kinoshita & Shimada, 2002;Saied et al, 2008;Shabbir et al, 1997), alkaloids (Kong et al, 2007), and volatile oils (Chowdhury et al, 2008). These leaves have been found to be useful for hepatoprotective (Sathaye et al, 2011), antidiabetic (Arulselvan et al, 2006;Tembhurne & Sakarkar, 2009, 2010b, antimicrobial (Gautam et al, 2012b;Sundaram, 2011), anti-inflammatory (Gupta et al, 2010;Mathur et al, 2011), wound healing (Patidar et al, 2010), and antidiarrheal (Mandal et al, 2010) purposes.…”

Section: Introductionmentioning

confidence: 99%

Chemical composition and evaluation of acute toxicological, antimicrobial and modulatory resistance of the extract ofMurraya paniculata

Menezes

Santana

Varela

et al. 2014

Pharmaceutical Biology

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Context: Murraya paniculata (Linn) JACK (Rutaceae) is used in traditional medicine in the treatment of diabetes, inflammation, and microbial disorders. Objective: This study determined the polyphenol composition and antimicrobial and acute toxicological activity of the hydroethanolic extract of M. paniculata leaves (EEMp). Materials and methods: Chemical composition was evaluated by the Folin-Ciocalteu and AlCl 3 assays and by HPLC-DAD. Antibacterial and modulatory activity was determined by the microdilution method. Toxicity was assessed with a single dose of EEMp administered orally at doses of 2000 and 5000 mg/kg body weight/day in male and female Swiss mice. Results: Total phenolic content of the EEMp samples varied from 66.5 to 396.8 mg gallic acid equivalent/g of extract and flavonoid content varied from 0.3 to 31.1 mg quercetin equivalent/g of extract. The principal component identified by HPLC-DAD assay was ellagic acid. The results of oral acute toxicity showed no mortality, changes in hematological parameters, or CNS and ANS toxicities in rats. Biochemical analysis showed a significant increase in glucose and glutamic oxaloacetic transaminase activity and reduction in triglycerides and cholesterol for 5000 and 2000 mg/kg doses, respectively, when compared with the control group. Histopathological evaluation showed no significant microscopic changes. EEMp showed essentially no antimicrobial activity, but when aminoglycosides were combined with EEMp their MIC was reduced. Conclusions: Significant effects were observed in the acute toxicity assay, but they had no clinical relevance. The results suggest that M. paniculata could be used as a source of natural products with antibacterial resistance-modifying activity, with lower toxicity.

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“…So far the leaves of this plant have been proved to possess significant antinociceptive, antibacterial, antifungal, and antioxidant [1,2]. It is widely used and has a good curative effect on treating superficial gastritis, erosive gastritis and intestinal metaplasia [3].…”

Section: Introductionmentioning

confidence: 99%

“…Murraya paniculata (L.) Jack, as an important Chinese traditional herb, has become an attractive subject for intensive pharmacological and chemical studies. So far the leaves of this plant have been proved to possess significant antinociceptive, antibacterial, antifungal, and antioxidant . It is widely used and has a good curative effect on treating superficial gastritis, erosive gastritis and intestinal metaplasia .…”

Section: Introductionmentioning

confidence: 99%

Graphene-encapsulated silica as matrix solid-phase dispersion extraction sorbents for the analysis of poly-methoxylated flavonoids in the leaves of Murraya panaculata (L.) Jack

Sun

Yang

et al. 2015

J. Sep. Science

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In this study, graphene-encapsulated silica was synthesized by a hydrothermal reduction strategy. The presence of silica in graphene was identified by Fourier-transform infrared spectrometry, X-ray diffraction and scanning electron microscopy. The graphene-encapsulated silica subsequently was used as adsorbent for matrix solid-phase dispersion extraction of poly-methoxylated flavonoids from the dried leaves of Murraya panaculata (L.) Jack. Compared with the other adsorbents (graphene, silica gel, C18 silica, neutral alumina, diatomaceous earth) and without any adsorbents, better results were obtained. Then a method for analysis of poly-methoxylated flavonoids was established by coupling matrix solid-phase dispersion extraction with ultra high performance liquid chromatography and UV detection. Compared with reflux extraction and ultrasonic extraction, the proposed method is quicker, more efficient and more environmental protection. Less than 10 min is needed from extraction to detection.

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Studies on in vitro Antibacterial, Antifungal Property and Antioxidant Potency of Murraya paniculata

Cited by 14 publications

References 15 publications

Effects of β-caryophyllene and Murraya paniculata essential oil in the murine hepatoma cells and in the bacteria and fungi 24-h time–kill curve studies

Effects of β-caryophyllene and Murraya paniculata essential oil in the murine hepatoma cells and in the bacteria and fungi 24-h time–kill curve studies

Chemical composition and evaluation of acute toxicological, antimicrobial and modulatory resistance of the extract ofMurraya paniculata

Graphene-encapsulated silica as matrix solid-phase dispersion extraction sorbents for the analysis of poly-methoxylated flavonoids in the leaves of Murraya panaculata (L.) Jack

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