STUDIES ON HOST-SPECIFICITY OF BACILLUS SUBTILIS-PHAGE (S-a), WITH SPECIAL REFERENCE TO ITS BEHAVIOUR TOWARD PROTOPLASTS

Fukuda, Shigeo

doi:10.2323/jgam.7.1

Cited by 6 publications

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“…Adsorption without concomitant killing has already been demonstrated by others to occur in bacilli (e.g., 13, 22, 29); and in our experimental system, 22a adsoibed with 50% efficiency to WT-B, L/22a, yet was unable to plaque on, or wipe-out, these bacilli (data not shown). Fukuda (12) reported that protoplasts of nonsusceptible bacteria were capable of adsorbing phage S-a, but showed no subsequent phage reproduction. He did not determine whether S-a killed the protoplast host.…”

Section: Resultsmentioning

confidence: 99%

Interaction of protoplasts, L forms, and bacilli of Bacillus subtilis with 12 strains of bacteriophage

Jacobson¹,

Landman²

1975

J Bacteriol

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The interaction of 12 phage strains with bacilli, protoplasts, and L forms of Bacillus subtilis 168 and with eight of its mutants and two of its lysogens is described qualitatively and quantitatively. After removal of the cell wall from B. subtilis 168, 11 of the 12 phage strains can still adsorb to the protoplasts, nine kill their wall-less host cells, and five multiply in the naked bacteria, forming plaques on L form lawns. Individual gene mutations can have similarly pleiotropic effects, strongly dependent upon the plating medium. Thus, the gta A mutation, which causes loss of glucosylation of the wall teichoic acid, results in loss of wall adsorption sites for 029 (but not membrane sites) and for X105. Phages X25, SP82G and oe can still adsorb to gta A bacilli and plaque in unstabilized and sorbitol-stabilized lawns of this mutant, but they can not plaque in sucrosestabilized lawns. The lysogenized wild type, B. subtilis 168 (SP02), also exhibits a pleiotropic pattern, showing different levels of resistance to phages SP02, X1, 4e, and 025. Its resistance pattern is very similar to that of wild-type protoplasts. On the basis of such patterns, the bacterial mutants and strain B. subtilis 168 strain 4)1

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Section: Resultsmentioning

confidence: 99%

Interaction of protoplasts, L forms, and bacilli of Bacillus subtilis with 12 strains of bacteriophage

Jacobson¹,

Landman²

1975

J Bacteriol

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Phage adsorption and productive lysis in stable protoplast type L‐forms of Bacillus subtilis and Streptomyces hygroscopicus

Gumpert

Zimmermann

Taubeneck

1986

J. Basic Microbiol.

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Transferable productive lysis in stable protoplast type L-form cells of Bacillus subtilis was produced by 6 phages out of 14 strains virulent for the parent B. subtilis 170 and 1997. Most of these phages lytic for L-forms show the phi 29 morphology characteristic for the smallest B. subtilis phages containing double-stranded DNA. Among 31 actinophages, 23 of which were virulent for Streptomyces hygroscopicus, only SLE 109 and phi c 31 gave productive infection of the stable protoplast type L-form of S. hygroscopicus NG 33--354. Electron microscopic investigation and treatment by DNAse demonstrated that infection of L-form cells is an adsorption-injection process, and that it is not caused by transfection of free phage DNA or endocytotic uptake of phage particles. Because in both stable L-forms cell wall biosynthesis is blocked irreversibly the results allow the conclusion that specific receptors must be localized in the cytoplasmic membrane for those phages producing transferable lysis in protoplast type L-forms. Localization of receptors for certain phages in the cytoplasmic membrane seems to occur in many Gram-positive bacteria, but not in Gram-negative bacteria.

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Studies on DNA‐mediated Interspecific Induction of Phage‐susceptibility in Bacilli

Fukuda

1962

J of Basic Microbiology

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Im Rahmen der linearisierten Theorie der nichtstationären positiven Säule der Niederdruckentladung werden Ausdrücke abgeleitet, mit deren Hilfe aus Meßdaten für das Dispersions‐ und Amplifikationsverhalten laufender Schichten auf Kenngrößen der Elementarprozesse im Plasma geschlossen werden kann. Zu diesen gehören die Ionenbeweglichkeit, die Wärmeleitfähigkeit der Elektronen sowie die Konzentrations‐ und Elektronentemperatur‐Abhängigkeit der Ionisations‐ und der Energieverlustrate. Außerdem liefert die Theorie für die Resonanzschichtweite bei der Grenzstromstärke einen Minimalwert, der nicht unterschritten werden darf.

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STUDIES ON HOST-SPECIFICITY OF BACILLUS SUBTILIS-PHAGE (S-a), WITH SPECIAL REFERENCE TO ITS BEHAVIOUR TOWARD PROTOPLASTS

Cited by 6 publications

References 0 publications

Interaction of protoplasts, L forms, and bacilli of Bacillus subtilis with 12 strains of bacteriophage

Interaction of protoplasts, L forms, and bacilli of Bacillus subtilis with 12 strains of bacteriophage

Phage adsorption and productive lysis in stable protoplast type L‐forms of Bacillus subtilis and Streptomyces hygroscopicus

Studies on DNA‐mediated Interspecific Induction of Phage‐susceptibility in Bacilli

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