Studies on Hg(II)-induced H2O2 formation and oxidative stress in vivo and in vitro in rat kidney mitochondria

Lund, Bert‐Ove; Miller, Dennis M.; Woods, James S.

doi:10.1016/0006-2952(93)90012-l

Cited by 281 publications

(145 citation statements)

References 39 publications

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“…The toxicologic characteristics of mercury (elemental, inorganic and organic forms) and its ability to react with and deplete free sulfhydryl groups are wellknown [20]. It is demonstrated that the administration of mercury as Hg (II) to rats results in increased hydrogen peroxide formation, glutathione depletion and lipid peroxidation in kidney mitochondria [21].…”

Section: Introductionmentioning

confidence: 99%

Probing the coordination properties of glutathione with transition metal ions (Cr2+,Mn2+,Fe2+,Co2+, Ni2+,Cu2+,Zn2+,Cd2+,Hg2+) by density functional theory

Liu

et al. 2014

J Biol Phys

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Probing the coordination properties of glutathione with transition metal ions (Cr 2+,were larger than that of M-N and M-O; for Cr 2+ complexes, most of the WBIs of M-O in complexes were higher than that of M-S and M-N. Furthermore, the changes in the electron configuration of the metal cations before and after chelate reaction revealed that Cu 2+ , Ni 2+ , Co 2+ and Hg 2+ had obvious tendencies to be reduced to Cu + , Ni + , Co + and Hg + during the coordination process.

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Section: Introductionmentioning

confidence: 99%

Probing the coordination properties of glutathione with transition metal ions (Cr2+,Mn2+,Fe2+,Co2+, Ni2+,Cu2+,Zn2+,Cd2+,Hg2+) by density functional theory

Liu

et al. 2014

J Biol Phys

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“…Hg 2+ also affects mitochondrial transport [12][13][14][15], cytoplasmic enzymes [2,16,17], and the cytoskeleton [18]. Hg 2+ -modification of Na + , K + , and Cl -permeability leads to changes in cell volume and loss of the regulatory volume decrease (RVD) [3,7,19,20].…”

Section: Introductionmentioning

confidence: 99%

Dynamic Effects of Hg2+-induced Changes in Cell Volume

Heo¹,

Meng²,

Sachs³

et al. 2008

Cell Biochem Biophys

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Using a microfluidic volume sensor, we studied the dynamic effects of Hg 2+ on hypotonic stressinduced volume changes in CHO cells. A hypotonic challenge to control cells caused them to swell but did not evoke a significant regulatory volume decrease (RVD). Treatment with 100 μM HgCl 2 caused a substantial increase in the steady-state volume following osmotic stress. Continuous hypotonic challenge following a single 10-min exposure to HgCl 2 produced a biphasic volume increase with a steady-state volume 100% larger than control cells. Repeated hypotonic challenges to cells exposed once to Hg 2+ resulted in a sequential approach to the same steady-state volume. Stimulation after reaching steady state caused a reduction in peak cell volume. Repeated stimulation was different than continuous stimulation resulting in a more rapid approach to steady state. Substituting extracellular Na + with impermeant NMDG + in the hypotonic solution produced a rapid RVD-like volume decrease and eliminated the Hg 2+ -induced excess swelling. The volume decrease in the presence of Hg 2+ was inhibited by tetraethylammonium and 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid disodium, blockers of K + and Cl -channels, respectively, suggesting that part of the Hg 2+ effect was increasing NaCl influx over KCl efflux. The presence of multiple phases of steadystate volume and their sensitivity to the stimulation history suggests that factors beyond solute fluxes, such as modification of mechanical stress within the cytoskeleton also plays a role in the response to hypotonic stress.

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“…However, it was later shown that, under physiological conditions in which intracellular thiols are depleted, the NmerA domain binds Hg 2ϩ and transfers it from ligands in cytoplasm to the catalytic core for reduction (12,13). Furthermore, it has been shown that Hg 2ϩ increases H 2 O 2 formation in mitochondria, leading to increased consumption of reduced glutathione (14). Taken together, these results strongly indicate that the pair of cysteines in the NmerA domain of mercuric reductases does have a functional role in vivo, enhancing mercuric ion detoxification by acting as an accessory pathway for delivery of the substrate to the active site (12).…”

mentioning

confidence: 99%

A Novel Mercuric Reductase from the Unique Deep Brine Environment of Atlantis II in the Red Sea

Sayed

Ghazy

Ferreira

et al. 2014

Journal of Biological Chemistry

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Background: Molecular features underlying enzyme function in extreme environments are poorly understood. Results: Identification of the basis for thermostability, halophilicity, and detoxification activity in a mercuric reductase from hot deep-sea brine. Conclusion: A small number of structural modifications accounts for the enzyme's robustness. Significance: This work defines novel adaptations that enable enzymes to cope with multiple abiotic stressors simultaneously.

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Studies on Hg(II)-induced H2O2 formation and oxidative stress in vivo and in vitro in rat kidney mitochondria

Cited by 281 publications

References 39 publications

Probing the coordination properties of glutathione with transition metal ions (Cr2+,Mn2+,Fe2+,Co2+, Ni2+,Cu2+,Zn2+,Cd2+,Hg2+) by density functional theory

Probing the coordination properties of glutathione with transition metal ions (Cr2+,Mn2+,Fe2+,Co2+, Ni2+,Cu2+,Zn2+,Cd2+,Hg2+) by density functional theory

Dynamic Effects of Hg2+-induced Changes in Cell Volume

A Novel Mercuric Reductase from the Unique Deep Brine Environment of Atlantis II in the Red Sea

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