Studies on Glomerular Basement Membrane. 1. Isolation and Chemical Analysis of Normal Glomerular Basement Membrane.

Molecular Sieve in Rat Tubular Basement Membrane as Revealed by Negative Staining

“…Ferwerda et al [8], Sato et al [27] and Krisko et al [11] also isolated TBM using series of mesh sieves of varing sizes for disruption of the tissue as well as for further purification. On the other hand, Cook and Pickering [6], Misra and Berman [16] and Carlson et al [4] perfused the kidney with magnetic iron oxide and separated tubuli from glomeruli using a magnet. How ever, these studies were limited either to the purification or to the chemical analysis of TBM .…”

“…Glomerular basement membrane (GBM) of the kidney is by far the most well known and extensively studied. Components of G B M have been analyzed by many workers both in normal [10,16,29,34] and diseased states [2,14,17,27], On the other hand, little is known concerning tubu lar basement membrane (TBM). TBM has been isolated by some workers [8,11,13,27], although their studies were mostly limited to analysis of the chemical composition.…”

Normal Glomerular Permeability and Its Modification by Minimal Change Nephrotic Syndrome

“…Thus, albumin is eluted before mannose from a column prepared from normal rat GBM, whereas the two compounds are eluted simultaneously from a column prepared from nephrotic rat GBM (22). In addition, the chemical composition of the GBM is altered in both human and experimental renal disease, and X-ray diffraction patterns suggest that there may be molecular rearrangement in this membrane with an increase in interstices between molecules (23)(24)(25). Similarly the clearances of macromolecules have been interpreted as indicating an increase in pore size in the GBM as the cause of proteinuria in nephrotic syndrome (26).…”

Cytochemical Studies of Ameloblasts and the Surface Layer of Enamel of the Rat Incisor at the Maturation Stage

“…The biochemical analyses of the basal lamina revealed that it partially consisted of collagenous components (MIZRA and BERMAN, 1966;KEFALIDES and WINZLER, 1966;KEFALIDES, 1967;DANIELS and CHU, 1975;CARLSON et al, 1978). KAHL and PEARSON (1967) and EVAN et al (1976), in fact, succeeded in digesting the basal lamina with collagenase.…”