Studies on Constituents of Fruit Body of Polyporus umbellatus and Their Cytotoxic Activity.

et al. 2010

Mycol Progress

Polyporus umbellatus is one of the precious medicinal fungi, with sclerotia used as a diuretic agent and antidote in China for many years. This has led to the present interest in producing sclerotia of P. umbellatus in the laboratory due to a decreased abundance in natural sources. Here, we investigated the determining factors for sclerotial formation in P. umbellatus. Five carbon sources, namely, maltose, fructose, glucose, sucrose and soluble starch with different initial pH values were evaluated for their effects on mycelial growth and sclerotial development of P. umbellatus. Maltose, fructose and glucose could induce sclerotial formation of P. umbellatus. Sucrose and soluble starch could stimulate growth of the fungus but had no effect on sclerotial formation. The most efficient sclerotial production occurred with maltose followed by fructose and a pH of 5. In addition, different macroscopically evident characteristics of sclerotial development of P. umbellatus induced by different carbon sources were also observed. Our findings could provide new insights into further research on sclerotial production in P. umbellatus under artificial cultivation.

Section: Introductionmentioning

confidence: 99%

Determination of optimal carbon source and pH value for sclerotial formation of Polyporus umbellatus under artificial conditions

Xing

et al. 2010

Mycol Progress

“…Anticancer activities of P. umbellatus have been demonstrated in animal studies (54)(55)(56) as well as in one clinical study with bladder cancer patients in China (57). Although the biological activity of P. umbellatus was associated with the presence of polysaccharides (58), the cytotoxic activity against leukemia cells was demonstrated with isolated triterpenes (polyporusterones) isolated from P. umbellatus (59). In addition, other ergostane-type ecdysteroids named polyporoids demonstrated anti-inflammatory activity in a TPA-induced inflammation in mice (60).…”

Section: Introductionmentioning

confidence: 99%

Novel medicinal mushroom blend suppresses growth and invasiveness of human breast cancer cells

Jiang

Slíva²

2010

Int J Oncol

Abstract. Mushrooms are an integral part of Traditional Chinese Medicine (TCM), and have been used for millennia to prevent or treat a variety of diseases. Currently mushrooms or their extracts are used globally in the form of dietary supplements. In the present study we have evaluated the anticancer effects of the dietary supplement, MycoPhyto ® Complex (MC), a novel medicinal mushroom blend which consists of a blend of mushroom mycelia from the species Agaricus blazei, Cordyceps sinensis, Coriolus versicolor, Ganoderma lucidum, Grifola frondosa and Polyporus umbellatus, and ß-1,3-glucan isolated from the yeast, Saccharomyces cerevisiae. Here, we show that MC demonstrates cytostatic effects through the inhibition of cell proliferation and cell cycle arrest at the G2/M phase of highly invasive human breast cancer cells MDA-MB-231. DNA-microarray analysis revealed that MC inhibits expression of cell cycle regulatory genes (ANAPC2, ANAPC2, BIRC5, Cyclin B1, Cyclin H, CDC20, CDK2, CKS1B, Cullin 1, E2F1, KPNA2, PKMYT1 and TFDP1). Moreover, MC also suppresses the metastatic behavior of MDA-MB-231 by the inhibition of cell adhesion, cell migration and cell invasion. The potency of MC to inhibit invasiveness of breast cancer cells is linked to the suppression of secretion of the urokinase plasminogen activator (uPA) from MDA-MB-231 cells. In conclusion, the MC dietary supplement could have potential therapeutic value in the treatment of invasive human breast cancer.

“…Grifola umbellata (Fries) is an edible mushroom and is also termed as Polyporus umbellatus or Chuling in other scientific reports [1,2]. The fruiting bodies of G. umbellata are commonly used for treating edema and promoting diuretic process [3].…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that G. umbellata possesses outstanding anti-tumor effects [4]. Ohsawa et al [1] reported that the constituents of G. umbellata showed cytotoxic activity on leukemia 1210 cell proliferation. You et al [5] used fruiting body extract for the treatment of liver cancer and the results indicated that Chuling has the potential to be an anticancer agent.…”

Section: Introductionmentioning

confidence: 99%

The use of additives as the stimulator on mycelial biomass and exopolysaccharide productions in submerged culture of Grifola umbellata

Huang

et al. 2009

Bioprocess Biosyst Eng

In this study, various additives including organic acids, alcohols, vegetable oils, surfactants and polymers were added in the cultural medium to investigate their stimulatory effects on Grifola umbellate mycelia growth and exopolysaccharide (EPS) production. It was found that the commonly used stimulatory additives, effective in other mushrooms' cultures, exhibited negative results in Grifola umbellata submerged culture. In contrast, the polymer additive, polyethylene glycol (PEG), displayed an effective stimulatory effect on both biomass and EPS productions. With the addition of PEG8 (molecular weight: 8,000 Da), the mycelial biomass production at day 12 was increased from 4.69 to 6.30 g/L, accounting for a 34% increase. Meanwhile, the EPS production was enhanced from 0.478 to 0.767 g/L, accounting for 60% increase.