Studies on complement-potentiated neutralizing antibodies (C′-PNAb) induced in rabbits inoculated with japanese encephalitis virus (JEV)

Iwasaki, Taku; Ogura, R

doi:10.1016/0042-6822(68)90007-x

Cited by 19 publications

(14 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…9. DISCUSSION The fact that the virus-neutralizing activity of IgM antibody frequently shows a high C' dependency [4,7,13,22,25] might give one an impression that the CRN activity of early immune sera, as observed in immunization of animals with herpes virus [4,24,25], may be ascribable to the early development of IgM antibody in advance of IgG antibody. The present experiments, extending our earlier observation [25], have helped amend such a misinterpretation and established that there does exist CRN IgG antibody in early sera and it plays an important role in qualifying the CRN nature of those sera.…”

Section: Physicochemical Properties Of Peaks I Ii and Ivmentioning

confidence: 99%

Complement Requirement of Neutralizing Antibodies in Different Classes of Immunoglobulin Appearing in Rabbits and Guinea Pigs after Primary and Booster Immunizations with Herpes Simplex Virus

Shinkai

Yoshino

1975

Japanese Journal of Microbiology

View full text Add to dashboard Cite

Rabbits and guinea pigs were immunized with herpes simplex virus and bled periodically. The sera were fractionated into slow IgG, fast IgG and IgM by DEAE‐cellulose column chromatography, and complement‐requiring (CRN) and nonrequiring neutralizing (N) antibody activities were estimated. In early sera of rabbits, the two IgG and IgM fractions possessed about equal CRN activities, although some animals showed a slightly lower activity in fast IgG. In guinea pigs, the early CRN activity resided mainly in slow IgG (7 S γ2). The early IgG antibody of guinea pigs differed from that of rabbits in that it resembled IgM in resistances to heating at 70 C and to 2‐mercaptoethanol. The level of CRN IgM antibody in rabbits declined following a peak reached in 2 to 3 weeks, whereas such a decline was never observed in guinea pigs. N IgG antibody was developed a few weeks after the first immunization in rabbits and much retarded in guinea pigs. In both species, booster immunization quickly evoked N antibody in the two IgG fractions and also CRN IgM antibody, but in the case of rabbits the IgM antibody disappeared soon. It is concluded that IgG plays an important role in humoral immunity from the initial stage of the immunization course.

show abstract

Section: Physicochemical Properties Of Peaks I Ii and Ivmentioning

confidence: 99%

Complement Requirement of Neutralizing Antibodies in Different Classes of Immunoglobulin Appearing in Rabbits and Guinea Pigs after Primary and Booster Immunizations with Herpes Simplex Virus

Shinkai

Yoshino

1975

Japanese Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…Investigations with rubella [26], Japanese encephalitis [13,24], vaccinia [23], bovine parainfluenza [17], Newcastle disease [21,35], respiratory syncytial [2], certain group B arbo [36,37] and simian herpes SA 8 viruses [29] have revealed occurrence of similar phenomena. However, the data presented have not necessarily coincided as to the extent to which the neutralizing activity of early serum is enhanced by C' as compared with that of late serum.…”

mentioning

confidence: 99%

Absence of an All‐or‐None Type of Complement Requirement in Early Neutralizing Antibody against Western Equine Encephalitis Virus

Yoshino

Morishima

Aoki

1971

Japanese Journal of Microbiology

View full text Add to dashboard Cite

It has been established that rabbits and guinea pigs immunized or infected with herpes simplex virus develop early neutralizing antibody which functions only in the presence of complement (C') [9,[40][41][42]. This antibody was named C'-requiring neutralizing (CRN) antibody [40]. Herpetic infections of man also stimulate the development of CRN antibody in advance of ordinary neutralizing (N) antibody, so that the detection of CRN antibody in an early serum sample can give a positive diagnosis [42], although this means of early diagnosis decreases in practicability in the case of repeated infections among adults [12,20] [36,37] and simian herpes SA 8 viruses [29] have revealed occurrence of similar phenomena. However, the data presented have not necessarily coincided as to the extent to which the neutralizing activity of early serum is enhanced by C' as compared with that of late serum. In the case of herpes virus, early CRN antibody frequently far exceeds N antibody in level and, as a consequence, enhancement by C' of the neutralizing activity of such a serum is almost of the all-or-none type. A similar fact has been noted with vaccinia [23], bovine parainfluenza [17] and simian her-

show abstract

“…There have been many papers reporting that the reaction rate of virus neutralization with IgG antibody follows a single hit reaction but the reaction with IgM antibody shows a departure from linearity (Dulbecco et al ., 1956;Mandel, 1961;Laff erty, 1963;Svehag et al, 1964;Iwasaki and Ogura, 1968) . However, virtually little information is available concerning the mode of neutralizing action of IgA globulin antibodies against viruses.…”

Section: Discussionmentioning

confidence: 99%

COMPARATIVE STUDIES ON THE MODE OF NEUTRALIZATION REACTION OF POLIOVIRUS TYPE 2 WITH SERUM IgG AND SECRETORY IgA FROM MOTHER'S MILK AND FECAL EXTRACT

Akao

Sasagawa

Shiga

et al. 1971

JJMSB

View full text Add to dashboard Cite

Studies on complement-potentiated neutralizing antibodies (C′-PNAb) induced in rabbits inoculated with japanese encephalitis virus (JEV)

Cited by 19 publications

References 44 publications

Complement Requirement of Neutralizing Antibodies in Different Classes of Immunoglobulin Appearing in Rabbits and Guinea Pigs after Primary and Booster Immunizations with Herpes Simplex Virus

Complement Requirement of Neutralizing Antibodies in Different Classes of Immunoglobulin Appearing in Rabbits and Guinea Pigs after Primary and Booster Immunizations with Herpes Simplex Virus

Absence of an All‐or‐None Type of Complement Requirement in Early Neutralizing Antibody against Western Equine Encephalitis Virus

COMPARATIVE STUDIES ON THE MODE OF NEUTRALIZATION REACTION OF POLIOVIRUS TYPE 2 WITH SERUM IgG AND SECRETORY IgA FROM MOTHER'S MILK AND FECAL EXTRACT

Contact Info

Product

Resources

About