Studies on clonogenic hemopoietic cells of vertebrate in space: problems and perspectives

Domaratskaya, E. I.; Michurina, Tatyana V.; Bueverova, E. I.; Bragina, E. Yu.; Nikonova, T.A.; Старостин, В. И.; Khrushchov, N. G.

doi:10.1016/s0273-1177(02)00394-0

Cited by 17 publications

(11 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They found that the number of stromal fibroblastic progenitor cells and hematopoietic progenitor cells in the rat bone marrow were significantly decreased when animals were exposed to spaceflight conditions. 4 Very few experiments at cellular level had been done due to the difficulties in performing cell culture in space.…”

Section: Letter To the Editormentioning

confidence: 99%

Real microgravity condition promoted regeneration capacity of induced pluripotent stem cells during the TZ‐1 space mission

et al. 2019

View full text Add to dashboard Cite

Induced pluripotent stem cells (iPSCs) are reprogrammed somatic cells that gained self‐renewal and differentiation capacity similar to embryonic stem cells. Taking the precious opportunity of the TianZhou‐1 spacecraft mission, we studied the effect of space microgravity (µg) on the self‐renewal capacity of iPSCs. Murine iPSCs carrying pluripotency reporter Oct4‐GFP were used. The Oct4‐EGFP‐iPSCs clones were loaded into the bioreactor and exposed to μg in outer space for 14 days. The control experiment was performed in identical device but on the ground in earth gravity (1 g). iPSCs clones were compact and highly expressed Oct4 before launch. In μg condition, cells in iPSC clones spread out more rapidly than those in ground 1 g condition during the first 3 days after launch. However, in 1 g condition, as the cell density increases, the Oct4‐GFP signal dropped significantly during the following 3 days. Interestingly, in μg condition, iPSCs originated from the spread‐out clones during the first 3 days appeared to cluster together and reform colonies that activated strong Oct4 expression. On the other hand, iPSC clones in 1 g condition were not able to recover Oct4 expression after overgrown. Our study for the first time performed real‐time imaging on the proliferation process of iPSCs in space and found that in μg condition, cell behaviour appeared to be more dynamic than on the ground.

show abstract

Section: Letter To the Editormentioning

confidence: 99%

Real microgravity condition promoted regeneration capacity of induced pluripotent stem cells during the TZ‐1 space mission

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The number of myeloid progenitor cells expanded 2.6‐ to 17.5‐fold on the ground compared to 0.9‐ to 7.0‐fold during the flight, and the number of erythroid progenitor cells expanded 2.0‐ to 4.1‐fold on the ground compared to the actual decline of the number of cells observed under microgravity (>83% reduction; Davis et al, ). In addition, the number of HSCs, monocyte‐macrophage precursors, and red blood cells decreased (Domaratakaya et al, ). It was shown that proliferation of bone marrow CD34 + cells is restrained after culture for 4–6 days in RWVB (rotating wall vessel bioreactor; Plett et al, ).…”

Section: Microgravity Inhibits Proliferation and Migration Of Hematopmentioning

confidence: 99%

Behavior of stem cells under outer‐space microgravity and ground‐based microgravity simulation

Zhang

Chen

et al. 2015

Cell Biology International

View full text Add to dashboard Cite

With rapid development of space engineering, research on life sciences in space is being conducted extensively, especially cellular and molecular studies on space medicine. Stem cells, undifferentiated cells that can differentiate into specialized cells, are considered a key resource for regenerative medicine. Research on stem cells under conditions of microgravity during a space flight or a ground-based simulation has generated several excellent findings. To help readers understand the effects of outer space and ground-based simulation conditions on stem cells, we reviewed recent studies on the effects of microgravity (as an obvious environmental factor in space) on morphology, proliferation, migration, and differentiation of stem cells.

show abstract

“…The number of stromal fibroblastic progenitors (CFC‐f) in the bone marrow of spaceflight rats statistically significantly decreases, in common with those of other progenitor cells, such as the erythrocyte lineages (BFU‐e and CFU‐e) (Colvin et al . 2002; Domaratskaya et al . 2002).…”

Section: Introductionmentioning

confidence: 99%

“…2005; Chen et al . 2006), and a few controversial results have been published recently (Domaratskaya et al . 2002; Merzlikina et al .…”

Section: Introductionmentioning

confidence: 99%

Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells

et al. 2007

View full text Add to dashboard Cite

show abstract

Studies on clonogenic hemopoietic cells of vertebrate in space: problems and perspectives

Cited by 17 publications

References 5 publications

Real microgravity condition promoted regeneration capacity of induced pluripotent stem cells during the TZ‐1 space mission

Real microgravity condition promoted regeneration capacity of induced pluripotent stem cells during the TZ‐1 space mission

Behavior of stem cells under outer‐space microgravity and ground‐based microgravity simulation

Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells

Contact Info

Product

Resources

About