Behavior of stem cells under outer‐space microgravity and ground‐based microgravity simulation

Zhang, Cui; Li, Liang; Chen, Jianling; Wang, Jinfu

doi:10.1002/cbin.10452

Cited by 30 publications

(28 citation statements)

References 31 publications

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“…In particular, cells seemed to have partially lost their typical spindle-like shape presenting a flattened form (white arrows, Figures 2D,F ). This effect is in line with previous studied carried out on stem cells in MG morphology ( Zhu et al, 2014 ; Zhang et al, 2015 ; Xue et al, 2017 ).…”

Section: Resultssupporting

confidence: 92%

Bioinspired Scaffold Action Under the Extreme Physiological Conditions of Simulated Space Flights: Osteogenesis Enhancing Under Microgravity

Avitabile

Fusco

Minardi

et al. 2020

Front. Bioeng. Biotechnol.

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Prolonged exposure to microgravity (MG) during long-duration space flights is known to induce severe dysregulation of osteoblast functions connected to a significant bone loss, similar to the condition induced by osteoporosis. Hence, we here present MG as a promising model to challenge the effectiveness of new scaffolds designed for bone regeneration in counteracting bone loss. To this end, we carried out an integrative study aimed to evaluate, in the extreme condition of Random Positioning Machine-simulated MG, the osteoinductive potential of nanocrystalline magnesium-doped hydroxyapatite/type I collagen composite scaffold (MHA/Coll), that we previously demonstrated to be an excellent tool for bone tissue engineering. Initially, to test the osteoinductive properties of our bioinspired-scaffold, MHA/Coll structure was fully characterized under MG condition and compared to its static counterpart. Human bone marrow-derived mesenchymal stem cells were used to investigate the scaffold biocompatibility and ability to promote osteogenic differentiation after long-duration exposure to MG (up to 21 days). The results demonstrate that the nanostructure of MHA/Coll scaffold can alleviate MG-induced osteoblast dysfunction, promoting cell differentiation along the osteogenic lineage, with a consequent reduction in the expression of the surface markers CD29, CD44, and CD90. Moreover, these findings were corroborated by the ability of MHA/Coll to induce the expression of genes linked to osteogenesis, including alkaline phosphatase and osteocalcin. This study confirmed MHA/Coll capabilities in promoting osteogenesis even in extreme long-term condition of MG, suggesting MG as an effective challenging model to apply in future studies to validate the ability of advanced scaffolds to counteract bone loss, facilitating their application in translational Regenerative Medicine and Tissue Engineering.

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Section: Resultssupporting

confidence: 92%

Bioinspired Scaffold Action Under the Extreme Physiological Conditions of Simulated Space Flights: Osteogenesis Enhancing Under Microgravity

Avitabile

Fusco

Minardi

et al. 2020

Front. Bioeng. Biotechnol.

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“…To some extent, MG cells resemble neural stem cells [6], which have been studied in space conditions by other groups. Murine neural stem cells, cultivated in a 3D bioreactor in space, maintained their ''stemness,'' even displayed upregulation of stem cell transcription factors Sox2 and Pax6, but proliferated slower than in 1 g and underwent neural differentiation more readily [15].…”

Section: Müller Gliamentioning

confidence: 99%

“…Research in this area utilizes a wide range of cell models, most common being cultured human or rodent stem cells. Taken together, results show the effects of real and simulated microgravity on stem cell migration, proliferation, and differentiation-key processes in tissue development and regeneration [1][2][3][4][5][6][7]. Original hardware is being developed to conduct such experiments onboard spacecrafts and in laboratories, and new ways of utilizing methods of cell culture in microgravity, as well as cells themselves, in bioengineering and regenerative medicine are being proposed [8,9].…”

Section: Introductionmentioning

confidence: 99%

Behavior of Stem-Like Cells, Precursors for Tissue Regeneration in Urodela, Under Conditions of Microgravity

Grigoryan

Radugina

2019

Stem Cells and Development

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We summarize data from our experiments on stem-like cell-dependent regeneration in amphibians in microgravity. Considering its deleterious effect on many tissues, we asked whether microgravity is compatible with reparative processes, specifically activation and proliferation of source cells. Experiments were conducted using tailed amphibians, which combine profound regenerative capabilities with high robustness, allowing an in vivo study of lens, retina, limb, and tail regeneration in challenging settings of spaceflight. Microgravity promoted stem-like cell proliferation to a varying extent (up to 2-fold), and it seemed to speed up source cell dedifferentiation, as well as sequential differentiation in retina, lens, and limb, leading to formation of bigger and more developed regenerates than in 1g controls. It also promoted proliferation and hypertrophy of Müller glial cells, eliciting a response similar to reactive gliosis. A significant increase in stem-like cell proliferation was mostly beneficial for regeneration and only in rare cases caused moderate tissue growth abnormalities. It is important that microgravity yielded a lasting effect even if applied before operations. We hypothesize on the potential mechanisms of gravity-dependent changes in stem-like cell behavior, including fibroblast growth factor 2 signaling pathway and heat shock proteins, which were affected in our experimental settings. Taken together, our data indicate that microgravity does not disturb the natural regenerative potential of newt stem-like cells, and, depending on the system, even stimulates their dedifferentiation, proliferation, and differentiation. We discuss these data along with publications on mammalian stem cell behavior in vitro and invertebrate regeneration in vivo in microgravity. In vivo data are very scarce and require further research using contemporary methods of cell behavior analysis to elucidate mechanisms of stem cell response to altered gravity. They are relevant for both practical applications, such as managing human reparative responses in spaceflight, and fundamental understanding of stem cell biology.

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“…Our previous studies have demonstrated that the conditions in outer space promote neuronal differentiation of NSCs [9]. As microgravity is one of the key factors of outer space [10,11], this present study investigated the influence of simulated microgravity on three-dimensional (3D) cultured NSCs with a dynamic 3D Rotary Cell Culture System (RCCS). The microgravity environment within the RCCS bioreactor is provided by using a random positioning machine that produces low fluid shear force.…”

Section: Introductionmentioning

confidence: 99%

The Rotary Cell Culture System increases NTRK3 Expression and Promotes Neuronal Differentiation and Migratory Ability of Neural Stem Cells Cultured on Collagen Sponge

Cui¹,

Yin

Zhao

et al. 2020

Preprint

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Background Recently, neural stem cell (NSC) therapy has shown promise for the treatment of many neurological diseases. Enhancing the quality of implanted cells and improving therapeutic efficacy are currently research hotspots. It has been reported that collagen sponge material provided sufficient room for cell growth in all directions, promoted the absorption of nutrients and removal of wastes. And also, the Rotary Cell Culture System (RCCS), which mimics the microgravity environment, can be used to culture cells for tissue engineering. Materials and methods We performed the mRNA and miRNA sequencing to elucidate the regulatory mechanism of NSCs cultured on the collagen sponge in RCCS system. The luciferase assay and Western blot revealed a direct regulatory role between let-7i-5p and NTRK3. And then, the neural differentiation maker Tuj1 and Map2 were detected by immunofluorescence staining. In the meantime, the migratory ability of NSCs was detected both in vitro and in spinal cord injury animals. Results In this study, we demonstrated that the expression of neurotrophic receptor tyrosine kinase 3 (NTRK3; also called TrkC) was elevated in NSCs cultured on collagen sponge in the RCCS system. Furthermore, increased NTRK3 expression was regulated by the downregulation of let-7i-5p. Compared to traditionally cultured NSCs, the NSCs cultured on collagen sponge in the RCCS system exhibited better neuronal differentiation and migratory ability, especially in the presence of NT-3. Conclusions As the biological properties and quality of transplanted cells are critical for therapeutic success, the RCCS system combined with collagen sponge culture system show promise for applications in clinical practice in the future.

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Behavior of stem cells under outer‐space microgravity and ground‐based microgravity simulation

Cited by 30 publications

References 31 publications

Bioinspired Scaffold Action Under the Extreme Physiological Conditions of Simulated Space Flights: Osteogenesis Enhancing Under Microgravity

Bioinspired Scaffold Action Under the Extreme Physiological Conditions of Simulated Space Flights: Osteogenesis Enhancing Under Microgravity

Behavior of Stem-Like Cells, Precursors for Tissue Regeneration in Urodela, Under Conditions of Microgravity

The Rotary Cell Culture System increases NTRK3 Expression and Promotes Neuronal Differentiation and Migratory Ability of Neural Stem Cells Cultured on Collagen Sponge

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