Studies on Carrier Substances of DNCB Contact Allergy

Nishioka, K; Aoki, Toshiyuki; Tashiro, Minoru

doi:10.1159/000252395

Cited by 23 publications

(15 citation statements)

References 6 publications

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“…This result confirms the observation of Parker and T urk [9], who have reported that the DNP microsomal fraction is statistically more antigenic than either the DNP mito chondrial fraction or the soluble conjugates in the cell sap. It has also been demonstrated by Nishioka et al [7] that the DNP microsomal fraction can elicit intense contact sensitivity. It is reasonable to assume from these find ings, if sensitization is by painting, the subcellular components of epidermal cells coupled in vivo with DNCB may play a great role in development of sensitivity.…”

Section: Discussionmentioning

confidence: 77%

The Induction of Delayed Sensitivity to 2.4-Dinitrophenyl Conjugates in Guinea Pigs Sensitized with DNCB

Nakagawa¹,

Tanioku²

1972

Dermatology

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Delayed reaction to DNP conjugate prepared in vitro from the extract of homologous epidermis could be clearly detected on intradermal injection in guinea pigs sensitized by epicutaneous application of DNCB and intradermal injection of Freund’s complete adjuvant to the previously applied area, but not to DNP guinea pig serum. The animals developed the most intense delayed reaction to DNP conjugate prepared from the subcellular small granule fraction of homologous epidermis. There was significant difference in the reactivity of this conjugate and DNP-soluble fraction of the epidermis. The animals also showed positive reactions to DNP conjugates prepared from the extracts of homologous heart, liver, kidney and heterologous (human) epidermis. This finding is explainable by cross-reactivity which exists between these conjugates and DNCB conjugated in vivo to autologous epidermis.

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Section: Discussionmentioning

confidence: 77%

The Induction of Delayed Sensitivity to 2.4-Dinitrophenyl Conjugates in Guinea Pigs Sensitized with DNCB

Nakagawa¹,

Tanioku²

1972

Dermatology

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“…DNP-Mic was prepared by the method described elsewhere [9,15], Briefly, depilated dorsal skin of guinea pigs was painted with 3% DNCB. 15 h later the epidermis was scraped and homogenized.…”

Section: Dinitrophenylated Epidermal Microsome (Dnp-mic)mentioning

confidence: 99%

Suppression of Contact Sensitivity by IgG1 Antihapten Antibody

Katayama¹,

Nishioka²

1983

Int Arch Allergy Immunol

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Sera obtained from guinea pigs sensitized with 2,4-dinitrofluorobenzene (DNFB) showed suppressive activity on the migration inhibitory factor (MIF) production by the lymph node cells obtained from DNFB-contact-sensitized guinea pigs. A similar suppressive effect was achieved by the sera of 2,4,6-trinitro-chlorobenzene- (TNCB-)sensitized guinea pigs. The suppressive factor in the sera was found to be IgG1 antihapten antibody. It suppressed MIF production dose-dependently during a period between 3 h before and 1 h after the antigen stimulation. Pretreatment of macrophages with IgG1 antihapten antibody abolished the MIF production of the lymph node cell. The antibody lost its suppressive activity after splitting off its Fc portion, and pretreatment of macrophages with Fc fragments of normal IgG1 interfered with the action of the IgG1 antibody. Therefore, the IgG1 antihapten antibody in the sera of contact-sensitized guinea pigs acted on macrophages through its Fc portion to inhibit MIF production by lymph node cells.

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“…RESULTS a) Conjugation with MDC Figure 4 shows the fluorescent spots of MDC and the reaction products on silica TLC 24 hours after mixing. Positive spots which are considered to be the reaction products were observed at R£ 0.09 to 0.59 with E [1], AE [3], DE [4], IE [5], AlE [7], IH [9] and catechol [13]. Also, definite fluorescent spots were observed at Rf 0.83 with AE [3] and AlE [7], which is an Rf value identical to that for acetyl monodansyl cadaverine (A-MDC).…”

Section: Conjugation Of Phenolic Compoundsmentioning

confidence: 99%

“…Figure 3 shows the fluorescent spots of DL and the reaction products on silica TLC three days after mixing. E [sample number I], AE [3], IE [5] and AlE [7] produced fluorescent spots at Rf 0.25 and 0040, and DE [4] at Rf 0.20 and 0.30. In contrast, ME [2] and MIE [6] did not produce any spots.…”

Section: Introductionmentioning

confidence: 99%

Conjugation of Some Phenolic Compounds With Amino Groups and Related Sensitization Potency

Osamura

Takéuchi

Urayama

et al. 1982

The Journal of Dermatology

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The conjugation of some phenolic compounds with ten dansyl amino acids was examined and the results indicated the importance of conjugation with the lysine residue. The conjugation of phenolic compounds with monodansyl cadaverine and cadaverine was also studied. The results obtained by these in vitro tests correlated well with those of animal sensitization tests and patch tests on patients with contact dermatitis.

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Studies on Carrier Substances of DNCB Contact Allergy

Cited by 23 publications

References 6 publications

The Induction of Delayed Sensitivity to 2.4-Dinitrophenyl Conjugates in Guinea Pigs Sensitized with DNCB

The Induction of Delayed Sensitivity to 2.4-Dinitrophenyl Conjugates in Guinea Pigs Sensitized with DNCB

Suppression of Contact Sensitivity by IgG1 Antihapten Antibody

Conjugation of Some Phenolic Compounds With Amino Groups and Related Sensitization Potency

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