Studies on Animal Collagenase

Abstract

During metamorphosis, the collagen layer (basement lamella) of the resorbing tail fin of bullfrog tadpole was markedly disintegrated, and was invaded by mesenchymal cells from below, which contained cytoplasmic vacuoles enclosing collagen fibrils. Participation of the locally produced collagenase in the process was discussed.By use of the tissue incubation method, collagenolytic activity was found in the wound tissues of the rabbit dorsal skin, and its significance in the wound healing process was discussed. Some of the repairing tissues after incubation were histologically examined.In the human stomach, high degree of collagenolytic activity was observed in the mucosa affected by gastritis after incubation, and there were several evidences that some kinds of cancerous tissues possessed a certain degree of the activity. The collagenolytic enzyme from the mucosa of gastritis was proved to have the characteristics of animal collagenase, previously obtained from tadpoles or other mammalian tissues. ACTA PATH. JAP. 24: 1-19, 1974. Characterization of the collagenolytic enzyme in the human stomachSmall pieces of the mucosa adjacent to ulcer of the stomach were cultivated a t 37°C for 0. USUKU r; .1.

Multiphoton microscopic imaging of rabbit dorsal skin

“…The changes of epidermal thickness and the morphological alteration in stratum corneum or stratum spinosum are tightly associated with skin diseases and barrier dysfunction such as the psoriasis, atopic dermatitis or contact dermatitis, and scars (Saulis et al ., ; Chung et al ., ; Mogford et al ., ; Bai et al ., ). Moreover, in the process of wound healing, the marginal epithelial layer of the wound became markedly thick after injury, covering the newly formed granulation tissue (Usuku et al ., ; Abidi et al ., ).…”

The Fine Structure of the Early Pelvic Fin Bud of the TroutsSalmo gairdneriandS. trutta fario

“…The involvement of such cells in collagen remodeling is also questionable since there is no evidence of collagen phagocytosis. An identical niesenchymal cell type is involved in collagen resorption during thyroxine induced tail resorption in the Rana tadpole (Usuku et al 1974) as evidenced by vacuoles within the hyloplasm containing collagen.…”