Studies on amylase inhibitors in some Egyptian legume seeds

Abstract

Amylase inhibitor activity was determined in four legume seeds which are widely consumed in Egypt. The effect of dehulling, heat treatment, soaking and germination were also assessed. The results showed that faba bean contained the highest activity of amylase inhibitor followed by cowpea, lentils, then chickpea. Dehulling resulted in raising the amylase inhibitor activities in all samples investigated, while heat treatment and cooking lowered it. Soaking for 10 h and germination eliminated completely the inhibitor from all samples.

Screening of Jordanian Flora for α-Amylase Inhibitory Activity

“…AA inhibitory activity was based on the starch-iodine method that was originally developed by Fuwa (1954) and later used by others for the determination of amylase activity in clinical samples (Close & Street, 1958) or in plant extracts (Shekib et al, 1988), with some modifications. Recently, the same principle of starch-iodine method was utilized in a microplate procedure for the determination of amylase enzyme activity.…”

Amylase Activities and Values in Hot and Cold Water Extracts of Pearl Millet

“…16) Reagents. The reagents used for the determination of amylase activity were as follows: Lugol s iodine.…”

“…Specific activity of amylase is expressed in U mg protein min. 16,17) Determination of cold and hot water extracts. Cold water extract (C.W.E.)…”

Capillary electrophoresis as a screening tool for alpha amylase inhibitors in plant extracts

“…AA inhibitory activity was based on the starch-iodine method that was originally developed by Fuwa (1954) and later employed by others for determination of amylase activity in clinical samples (Close and Street, 1958) or in plant extracts (Shekib et al, 1988) with some modifications. Recently the same principle of starch-iodine method was utilized in a microplate procedure for determination of the activity of the amylase enzyme.…”

“…Few methods for detecting AA inhibitors have been reported. These include spectroscopic (Fuwa, 1954;Shekib et al, 1988;Soud et al, 2002) and recently gel electrophoresis methods (Fontanini et al, 2007). However, the reported techniques still require relatively long run times which does not facilitate screening large number of plant extracts.…”