Screening of Jordanian Flora for α-Amylase Inhibitory Activity

Abstract

Thirty-five plant species that belong to different families were tested for α-amylase inhibitory activity. Four of the screened plants exhibited significant (more than 80%) α-amylase inhibitory activity. IC 50 values for these plants were estimated based on the dried crude extract and found to be 0.08, 0.13, 0.32, and 0.47 mg/ml, for Osyris alba L. (Santalaceae), Sarcopotarium spinosum L. (Rosaceae), Hypericum triquetrifolium Turra (Hypericaceae) and Arbutus andrachne L. (Ericaceae), respectively. Amylase inhibitory activity was also evaluated in vivo using female rats. Results showed that the tested extracts had a favorable effect on blood glucose level after ingestion of a high dose of sucrose. The most potent extract in controlling the surge of glucose after sucrose ingestion appeared to be O. alba which also exhibited the highest α-amylase inhibitory activity in vitro.

In vitro and in vivo acute antihyperglycemic effects of five selected indigenous plants from Jordan used in traditional medicine

“…Hamdan and Afifi (2008) highlighted the favourable effect of Sarcopoterium spinosum on controlling the surge of blood glucose level after the ingestion of high sucrose in rats. This potency was further verified by in vitro ␣-amylase inhibitory effect with an IC 50 of 0.13 mg/ml (Hamdan and Afifi, 2008). Remarkably, Sarcopoterium spinosum -based dual inhibitory efficacy of ␣-amylase and ␣-glucosidase in vitro was confirmed by significant acute antihyperglycemic trends in starch-fed rats.…”

Antidiabetic effects of natural plant extracts via inhibition of carbohydrate hydrolysis enzymes with emphasis on pancreatic alpha amylase

“…Some of these animal studies were focused on analyzing the reduction in blood glucose levels, after an oral starch tolerance test (OSTT) in which a decline in starch digestion and absorption was expected (31,32,44,149). In some other studies, in contrast, an oral sucrose or glucose tolerance test was performed (150)(151)(152). Nevertheless, the in vitro amylase inhibitory activities of some extracts were not potent enough to produce an in vivo response (153).…”

Characterization of Chemical Components in Organs Emissions of Two <i>Salvia dominica</i> L. Populations from Jordan

“…of many new sesterterpenoids 11,12 with interesting tubulin tyrosine lignase inhibition 11,13 . Additionally, the extract of the plant showed interesting anti-tumor activity against MCF-7 cancer cell lines and α-amylase inhibitory activity 14 .…”