Studies on aerial parts of Artemisia pallens wall for phenol, flavonoid and evaluation of antioxidant activity

Abstract

Herbs have been used for medicinal purposes for centuries. According to recent investigations, they may help reduce the risk of chronic diseases, cardiovascular disease, and cancer due to antioxidant properties, which in turn can be attributed to the various phytoconstituents. With this intention, evaluation of antioxidant activity was performed. Methanol extract of aerial parts of Artemisia pallens Wall was screened for its antioxidant activity due to phenolic and flavonoid contents, by employing radical scavenging assays; 2,2 –diphenyl, 1-picryl hydrazyl (DPPH) and nitric oxide. Ascorbic acid was used as a standard. Quantitative determination of phenols and flavonoids were carried out using spectrophotometric method. Total flavonoid content was determined as quercetin equivalent and total phenolic content was determined as pyrocatechol equivalent using Folin-Ciocalteu reagent. Plant produces more phenolic compounds than flavonoids. IC50 value of methanol extract for DPPH free radical scavenging activity was found to be 292.7 μg, whereas for nitric oxide it was 204.61 μg. The result obtained in the present study indicates that the aerial parts of this plant are a rich source of natural antioxidants

Synergistic antioxidant action of Phikud Navakot ameliorates hydrogen peroxide-induced stress in human endothelial cells

“…Our results suggested that almost all herbal ingredients, i.e., AD, AP, LC, NJ, PK, SC, and TC, served as scavengers of specific ROS and RNS, which was further supported by previous evidence 17, 18, 19, 20, 21, 22, 23, 24. To the best of our knowledge, the scavenging properties of AP against O 2 •− and of SC against OH • have not been reported elsewhere.…”

“…Our results suggested that almost all herbal ingredients, i.e., AD, AP, LC, NJ, PK, SC, and TC, served as scavengers of specific ROS and RNS, which was further supported by previous evidence. 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 To the best of our knowledge, the scavenging properties of AP against O 2 •− and of SC against OH • have not been reported elsewhere. It can be observed that, with the exception of TC, when individually assayed for their antioxidant activities and cytotoxicity, both the hydroethanolic and the water extract of each herbal ingredient in PN appeared to be less active than the polyherbal combination of PN.…”

Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L.

“…The antioxidant potential in various medicinal plants has been shown mainly due to phenolic compounds [11][12][13][14][15][16]. Likewise, different species of the genus Artemisia including A. absinthium L. have shown phenolics associated antioxidant activities [4,[17][18][19]. Furthermore, the importance of flavonoids as antioxidants and their role in antimalarial and anticancer activities of A. annua has been reviewed [20].…”

Comparison of Three Herbal Plants for Best Free Radical Scavenging Activity

“…Several previous studies have reported that it is used as antifungal, antiviral, and antimalarial agent. It is used to treat diabetes mellitus, rheumatoid arthritis and lupus nephritis [3]. The second plant, M. hortensis belongs to family Lamiaceae, finds its place in religious ceremonies and has several uses in therapeutic remedies, insecticidal effect due to terpenes, and carries several traditional uses [4].…”