Studies on Active Substances in Herbs Used for Hair Treatment. I. Effects of Herb Extracts on Hair Growth and Isolation of an Active Substance from Polyporus umbellatus F.

Inaoka, Yasunori; Shakuya, Akemi; Fukazawa, Hidesuke; Ishida, Hideyuki; Nukaya, Haruo; Tsuji, Kiichiro; Kuroda, Hideyo; Okada, Masanori; Fukushima, Makoto; Kosuge, Takuo

doi:10.1248/cpb.42.530

Cited by 19 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with earlier findings (Inaoka et al, 1994), the *L‐values of the injection sites correlated well with hair growth. Mice were subcutaneously injected FGF‐5 and/or the P3 peptide as described in the Materials and Methods, and skin pigmentation at the injected sites was measured to assess hair growth (Slominski et al, 1994).…”

Section: Resultssupporting

confidence: 91%

Decapeptide with fibroblast growth factor (FGF)‐5 partial sequence inhibits hair growth suppressing activity of FGF‐5

Ito

Saitoh

Fujita

et al. 2003

Journal Cellular Physiology

View full text Add to dashboard Cite

Earlier studies demonstrated that knock-out of fibroblast growth factor-5 gene (Fgf-5) prolonged anagen VI phase of hair cycle, resulting long hairs in the mice. We showed the activities on hair growth of the two Fgf-5 gene products, one of which, FGF-5 suppressed hair growth by inhibiting anagen proceeding and inducing the transition from anagen to catagen, and FGF-5S, a shorter polypeptide with FGF-5-antagonizing activity translated from alternatively spliced mRNA, suppressed this activity of FGF-5. As the results suggested that FGF-5 antagonist would increase hair growth, we synthesized various peptides having partial sequences of human FGF-5 and FGF-5S and determined their FGF-5 antagonist activity. Among them, a decapeptide designated P3 (95-VGIGFHLQIY-104) that aligns with receptor binding sites of FGF-1 and FGF-2 suppressed FGF-5-induced proliferation of BALB/3T3 A31 and NIH/3T3 murine fibroblasts, and FGF receptor-1c (FGFR-1c)-transfected Ba/F3 cell line (FR-Ba/F3 cells). IC50s of this peptide on these cell proliferations were 64, 28, 146 microM, respectively. On the other hand, IC50 of this peptide on binding of FGF-5 to the FGFR-1(IIIc)/Fc chimera was 483 microM. Examination in dorsal depilated mice revealed that the P3 peptide reduced the activity of FGF-5 to recover hair pigmentation and hair follicle lengths. The classification of histologically observed skin sections showed FGF-5-induced delations of anagen procedure had reduced by the P3 peptide. The anti-Ki67 antibody staining of hair follicles was inhibited by administration of FGF-5, and this inhibition by FGF-5 was recovered by administration of the P3 peptide. The P3 peptide alone did not affect hair follicle length and hair cell proliferation. These results indicate that the decapeptide antagonized FGF-5 activity in vivo, and reduced the inhibition of FGF-5 in hair growth, confirming that FGF-5 inhibitors are promising substances against hair loss and/or for promoting hair growth.

show abstract

Section: Resultssupporting

confidence: 91%

Decapeptide with fibroblast growth factor (FGF)‐5 partial sequence inhibits hair growth suppressing activity of FGF‐5

Ito

Saitoh

Fujita

et al. 2003

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…16,17) Direct protection of the RBC membrane from free radical attack as observed in the present study would provide an important pathophysiological basis for putting to use the helpful hemorrheological, anti-atherosclerotic and anti-inflammatory effects of polyporusterone A, polyporusterone B and Chuling. …”

Section: Discussionmentioning

confidence: 57%

Inhibitory Effects of Triterpenes Isolated from Chuling (Polyporus umbellatus FRIES) on Free Radical-Induced Lysis of Red Blood Cells

Sekiya

Hikiami

Nakai

et al. 2005

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Since anti-oxidative activitiy may play an important role in the mechanisms of atherogenesis, inflammation and aging, we have searched for new anti-oxidative substances among natural products. We have previously reported that Hoelen (Poria cocos WOLF) was found to have remarkable inhibitory effect on hemolysis induced by 2,2-azo-bis(2-amidinopropane)dihydrochloride (AAPH), an azo free-radical initiator. 1)Polyporus umbellatus FRIES is a saprophytic fungus growing on withered beech and maple trees roots. Its sclerotium, properly called Chuling, is a crude drug commonly used because of its diuretic effect, like Hoelen. Moreover, Chuling belongs to Basidiomycetes in Eumycetes, also like Hoelen.In this study, we examined the effect of Chuling on the membrane oxidative process. For that purpose, water extract powder was assayed and fractionated in order to isolate the principles responsible for the activity. The effects of Chuling on red blood cells (RBC) hemolysis induced by an azo freeradical initiator in healthy volunteers, and on scavenging reactive oxygen species (ROS) was examined by means of electron spin resonance (ESR) in the present study.Furthermore, we hypothesized that ingestion of Chuling would strengthen the antioxidative effect of plasma if the responsible compounds in Chuling could be absorbed and circulated in blood. Therefore, the present study was to examine the effect on plasma antioxidative activity after a gavagedose of Chuling in rats. MATERIALS AND METHODS Extraction and Fractionation of ChulingDried, ground sclerotia (1.0 kg) of Polyporus umbellatus FRIES (Chuling) were commercially purchased in China and extracted with water (8 l) under reflux for 2 h. The extract was filtered using a stainless steel filter (pore size 150 mm) and lyophilized to afford an aqueous extract (18.5 g). An aliquot of the extract (9.59 g) was first dissolved in water (100 ml), and ethanol was gradually added to a final concentration of 80%. After centrifugation, the precipitate was collected and dried under reduced pressure (P-fraction). The supernatant was fractionated using Diaion HP20 (500 ml, Mitsubishi Chemical Co., Japan), and eluted with 2 l of water (W-fraction), 50% aqueous methanol (50M-fraction), and then methanol (M-fraction), successively. These eluates were concentrated under reduced pressure to yield three fractions. The yields of P-, W-, 50M-, and M-fraction from 9.59 g of aqueous extract were 5.14, 3.40, 0.68, and 0.36 g, respectively. The aqueous extract and their fractions were used as test drugs for the estimation of anti-hemolytic activity.Isolation and Identification of Compounds from Chuling Dried, ground sclerotia (10.0 kg) of Polyporus umbellatus were extracted with methanol (20 l) under reflux for 2 h. The extract was filtered and concentrated to afford a methanolic extract (75 g). The methanolic extract was chromatographed over Diaion HP20 (1.3 l, Mitsubishi Chemical, Tokyo), and eluted with 6 l of water, 50% aqueous methanol, and then methanol, successively. The methanolic fraction (6.65...

show abstract

“…The significant dynamic segments are polysaccharides and steroidal mixes. Ethanolic extricate were accounted for to advance hair development in mice, and 3, 4dihydroxybenzaldehyde was secluded as a functioning segment 28 . A later report confined 3 hair regrowth substances, acetosyringone and polyporusterone An and B 29 .…”

Section: Polyporus Umbellatusmentioning

confidence: 99%

A Review on Hair Growth Regulator

Bharti

Shrivastav

Abid

et al. 2020

J. Drug Delivery Ther.

View full text Add to dashboard Cite

This review presents an overview on plants identified to possess hair growth activity in various ethno-botanical studies and surveys of tradition medicinal plants. There are various causes for hair loss and the phenomenon is still not fully understood. The treatments offered include both natural or synthetic products to treat the condition of hair loss (alopecia), nonetheless natural products are continuously gaining popularity mainly due to their fewer side effects and better formulation strategies for natural product extracts. Plants have been widely used for hair growth promotion since ancient times as reported in Ayurveda, Chinese and Unani systems of medicine. This review covers information about different herbs and herbal formulation that are believed to be able to reduce the rate of hair loss and at the same time stimulate new hair growth. A focus is placed on their mechanism of action and the review also covers various isolated phytoconstituents possessing hair growth promoting effect. Keywords: Alopecia, ayurveda, hair, herbal formulation.

show abstract

Studies on Active Substances in Herbs Used for Hair Treatment. I. Effects of Herb Extracts on Hair Growth and Isolation of an Active Substance from Polyporus umbellatus F.

Cited by 19 publications

References 0 publications

Decapeptide with fibroblast growth factor (FGF)‐5 partial sequence inhibits hair growth suppressing activity of FGF‐5

Decapeptide with fibroblast growth factor (FGF)‐5 partial sequence inhibits hair growth suppressing activity of FGF‐5

Inhibitory Effects of Triterpenes Isolated from Chuling (Polyporus umbellatus FRIES) on Free Radical-Induced Lysis of Red Blood Cells

A Review on Hair Growth Regulator

Contact Info

Product

Resources

About