Studies on ACTH action in perfused bovine adrenals: The site of action of ACTH in corticosteroidogenesis

Stone, David A.; Hechter, Oscar

doi:10.1016/0003-9861(54)90501-9

Cited by 448 publications

(80 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this process, cholesterol is converted to the final hormone products via a series of enzymatic reactions which are mostly catalyzed by membranebound enzymes [6]. The initial steps of this conversion are occurring in the mitochondria and lead to the formation of pregnenolone [7]. It is generally accepted that these steps are rate-limiting in steroidogenesis [8].…”

Section: Introductionmentioning

confidence: 99%

Sterol-phospholipid interactions in model membranes Effect of polar group substitutions in the cholesterol side-chain at C20 and C22

Gallay

Kruijff

Demel

1984

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

The interactions of phospholipids with four different cholesterol derivatives substituted with one OH or one keto group at position C2o or C22 of the side-chain were studied. The derivatives were the 22,R-hydroxy; 22,S-hydroxy; 22-keto-and 20,S-hydroxycholesterol. Two aspects of the interactions were investigated: (1) the effect of the cholesterol derivatives on the gel--, liquid crystalline phase transition of dipalmitoylphosphatidylcholine (DPPC) and of dielaidoyiphosphatidylethanolamine (DEPE) monitored by differential scanning calorimetry and (2) The effect on the lamellar--, hexagonal H n phase transition of DEPE monitored by DSC and by 3t P-NMR to determine structural changes. The gel ---, liquid crystalline phase transition was affected by the cholesterol derivatives to a much larger extent in the case of DPPC than of DEPE. In both cases, there was a differential effect of the four derivatives, the 22,R-hydroxycholesterol being the less effective. In DPPC-sterol 1 : 1 systems, 22,R-hydroxycholesterol does not suppress the melting transition, the AH values becomes 7.1 kcal. mol-1 as compared to 8.2 kcal. mol-1 for the pure lipid. 22,S-OH cholesterol has a much stronger effect (AH = 3.1 kcai. tool-1) and 22-ketocholesterol suppresses the transition completely. In DEPE mixtures of all these compounds, the melting transition of the phospholipid is still observable. The transition temperature was shifted to lower values (-13.5°C in the presence of 20,S-OH cholesterol). The AH of the transition was lowered by these compounds except in DEPE-22,R-OH cholesterol mixtures and the cooperativity of the transition (reflected by the width at half peak height) was reduced. The lameilar --, hexagonal H t~ phase transition was also affected by the presence of these cholesterol derivatives. The transition temperature value was depressed with all these compounds. 20,S-OH cholesterol was the most effective followed by 22,R-OH cholesterol. The AH of the transition was not strongly affected. The molecular intedacial properties of these derivatives were studied by the monomolecular film technique. It is most likely that 22,R-OH cholesterol due to the hydroxyl groups at the * To whom correspondence should be sent at (present address): E.R. No. 64 du Centre National de la Recherche Scientifique, Unit~ d'Enseignements et de Recherche Biom~dicale des Saints-P~res, 45, rue des Saints-P~res, 75270 Paris C~dex 06, France. Abbreviations: DEPE, 1,2-dielaidoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; EDTA, ethylenediaminetetraacetic acid; 22,R-OH cholesterol, (22,22,(22,20, 22-ketocholesterol, 5-cholestene-3fl-ol-22-one; cytochrome P-450sc¢, cytochrome P-450 specific for the side-chain cleavage of cholesterol.0005-2736/84/$03.00 © 1984 Elsevier Science Publishers B.V. 973~-and 22,R-positions orients with the sterol nucleus lying flat at the air/water interface, since the compression isotherm...

show abstract

Section: Introductionmentioning

confidence: 99%

Sterol-phospholipid interactions in model membranes Effect of polar group substitutions in the cholesterol side-chain at C20 and C22

Gallay

Kruijff

Demel

1984

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

show abstract

“…Cholesterol side chain cleavage (SCC) activity is enhanced by the action of ACTH [3] while subsequent steps in the production of corticosteroids, including the 1 l/3-hydroxylase activity, remain unaffected.…”

Section: Introductionmentioning

confidence: 99%

Spin state changes in cytochrome P‐450 associated with cholesterol side chain cleavage in bovine adrenal cortex mitochondria

1971

View full text Add to dashboard Cite

“…The substantial decrease in corticosterone response to ACTH in these rats may be correlated with an elevated rate of ACTH secretion which might have already stimulated regenerating adrenals (Fortier and DeGroot, 1959). Also, it may be related to the poor recovery of cholesterol sidechain-cleaving activity in regenerating adrenals (approximately 50% of the control at 20 days).It should be recalled here that the cholesterol sidechaincleaving reaction has been known to be stimulated by ACTH (Stone and Hechter, 1954;Hall, 1964: Karaboyas and. The partly impaired capacity for steroid biosynthesis and response to ACTH under in vitro incubation conditions have also been reported by Birmingham et al(1965).…”

Section: Plasma Corticosterone Levels In Enucleated Ratsmentioning

confidence: 87%

“…Because of the importance of adrenocortical mitochondria in the biosynthetic pathway of steroid hormones (Stone and Hechter, 1954;Halkerstone et al, 1961)an electron microscopic examination of mitochondria in regenerating adrenals have been performed in our laboratory and the data analysis is now under progress.…”

Section: Plasma Corticosterone Levels In Enucleated Ratsmentioning

confidence: 99%

Studies on Regenarating Adrenal Cortex 1

Ichii¹,

Yago²,

Kobayashi³

et al. 1968

Endocrinol Japon

View full text Add to dashboard Cite

Studies on ACTH action in perfused bovine adrenals: The site of action of ACTH in corticosteroidogenesis

Cited by 448 publications

References 12 publications

Sterol-phospholipid interactions in model membranes Effect of polar group substitutions in the cholesterol side-chain at C20 and C22

Sterol-phospholipid interactions in model membranes Effect of polar group substitutions in the cholesterol side-chain at C20 and C22

Spin state changes in cytochrome P‐450 associated with cholesterol side chain cleavage in bovine adrenal cortex mitochondria

Studies on Regenarating Adrenal Cortex 1

Contact Info

Product

Resources

About