Studies on 1-alkylamine adduct formation in electrospray ionization mass spectrometry for quantitative analysis

Teshima, Koichiro; Yoneyama, T; Kondo, Takahiro

doi:10.1016/j.jpba.2008.03.025

Cited by 4 publications

(3 citation statements)

References 11 publications

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“…This finding indicates that appropriate additives can reduce the need for numerous ILIS, presenting a substantial advantage in method development. Other studies have incorporated additives into the mobile phase, which imposes certain limitations regarding concentration and compatibility with mass spectrometry and the chromatography column. ,− The approach described herein not only simplifies and reduces costs (the additive is directly added to the sample rather than the mobile phase) but also offers greater flexibility with reversed-phase columns and mass spectrometry. This flexibility stems from their inert nature, high solubility in the mobile phase, and ease of rapid removal to waste.…”

Section: Resultsmentioning

confidence: 99%

Mitigating Matrix Effects in Groundwater: A Simple and Versatile Method for Quantifying Contaminants in Groundwater Using Online SPE-UHPLC-MS/MS

Orlando,

Augusti,

Nori de Macedo

et al. 2023

ACS EST Water

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This manuscript describes a novel and simple strategy for optimizing the determination of contaminants in groundwater using online solid-phase extraction (SPE) coupled with ultrahigh-performance liquid chromatography (UHPLC) and tandem mass spectrometry (MS/MS). The methodology optimization process considered several factors: analyte sorption in the syringe filter material, SPE conditions for effective cleanup, maximum injection volume, and spiking samples with additives to mitigate matrix effects. Matrix effects posed the greatest challenge, which was addressed by adding a few microliters of additives (citric acid and hydroxylamine) directly into the groundwater samples. This simple yet advantageous approach was also demonstrated with UHPLC coupled to high-resolution mass spectrometry without employing SPE. Under the optimized conditions, ten contaminants were evaluated, yielding excellent results at detected levels ranging from parts per billion (ppb) to parts per trillion (ppt), even without using isotopically labeled internal standards. These findings highlight the importance of investigating possible experimental issues that have a critical influence on the performance of analytical protocols. Finally, this work provides valuable insights for other researchers working on LC-MS/MS methods for determining organic contaminants in groundwater.

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Section: Resultsmentioning

confidence: 99%

Mitigating Matrix Effects in Groundwater: A Simple and Versatile Method for Quantifying Contaminants in Groundwater Using Online SPE-UHPLC-MS/MS

Orlando,

Augusti,

Nori de Macedo

et al. 2023

ACS EST Water

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“…In addition to the spectral peak centered around 4960.62 Da, there were two peaks centered near 4982.30 Da and 5005.52 Da that represent the sodium adducts of the peptide [M + Na] + . This is typical of the mass spectra of peptides that absorb alkaline atoms from the solution or glass storage containers and does not represent degradation or chemical modification of the parent peptide [ 22 ]. A similar mass spectral pattern was observed in samples stored for three years (Fig.…”

Section: Resultsmentioning

confidence: 99%

Structural and functional stability of the sulfur-free surfactant protein B peptide mimic B-YL in synthetic surfactant lipids

et al. 2021

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Background Optimal functionality of synthetic lung surfactant for treatment of respiratory distress syndrome in preterm infants largely depends on the quality and quantity of the surfactant protein B (SP-B) peptide mimic and the lipid mixture. B-YL peptide is a 41-residue sulfur-free SP-B mimic with its cysteine and methionine residues replaced by tyrosine and leucine, respectively, to enhance its oxidation resistance. Aim Testing the structural and functional stability of the B-YL peptide in synthetic surfactant lipids after long-term storage. Methods The structural and functional properties of B-YL peptide in surfactant lipids were studied using three production runs of B-YL peptides in synthetic surfactant lipids. Each run was held at 5 °C ambient temperature for three years and analyzed with structural and computational techniques, i.e., MALDI-TOF mass spectrometry, ATR-Fourier Transform Infrared Spectroscopy (ATR-FTIR), secondary homology modeling of a preliminary B-YL structure, and tertiary Molecular Dynamic simulations of B-YL in surfactant lipids, and with functional methods, i.e., captive bubble surfactometry (CBS) and retesting in vivo surface activity in surfactant-deficient young adult rabbits. Results MALDI-TOF mass spectrometry showed no degradation of the B-YL peptide as a function of stored time. ATR-FTIR studies demonstrated that the B-YL peptide still assumed stable alpha-helical conformations in synthetic surfactant lipids. These structural findings correlated with excellent in vitro surface activity during both quasi-static and dynamic cycling on CBS after three years of cold storage and in vivo surface activity of the aged formulations with improvements in oxygenation and dynamic lung compliance approaching those of the positive control surfactant Curosurf®. Conclusions The structure of the B-YL peptide and the in vitro and in vivo functions of the B-YL surfactant were each maintained after three years of refrigeration storage.

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“…6 Furthermore, Zhao et al, 7 Mortier et al, 2 and Karnes' group 8,9 reported the same usefulness of alkylamines for simvastatin, paclitaxel, and docetaxel, respectively. Teshima et al investigated an application of 1-alkylamines for quantitative analysis using LC/ESI-MS, [10][11][12] while other chemicals have been studied as well. 13,14 The aforementioned mobile-phase additive is useful for regulating the multimer and improving the sensitivity of detection.…”

Section: Introductionmentioning

confidence: 99%

Elimination Technique for Alkali Metal Ion Adducts from an Electrospray Ionization Process Using an On-line Ion Suppressor

et al. 2010

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Studies on 1-alkylamine adduct formation in electrospray ionization mass spectrometry for quantitative analysis

Cited by 4 publications

References 11 publications

Mitigating Matrix Effects in Groundwater: A Simple and Versatile Method for Quantifying Contaminants in Groundwater Using Online SPE-UHPLC-MS/MS

Mitigating Matrix Effects in Groundwater: A Simple and Versatile Method for Quantifying Contaminants in Groundwater Using Online SPE-UHPLC-MS/MS

Structural and functional stability of the sulfur-free surfactant protein B peptide mimic B-YL in synthetic surfactant lipids

Elimination Technique for Alkali Metal Ion Adducts from an Electrospray Ionization Process Using an On-line Ion Suppressor

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