Studies of Tocopherol Deficiency in Infants and Children&lt;subtitle&gt;I. Hemolysis of Erythrocytes in Hydrogen Peroxide&lt;/subtitle&gt;

Gordon, H. McL.

doi:10.1001/archpedi.1955.04030010671002

Cited by 55 publications

(27 citation statements)

References 27 publications

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“…This concept derives support from clinical studies in tocopherol-deficient pa-7 8 tients with abetalipoproteinemia, whose increased autohemolysis is corrected within 24 hr of the administration ocytes during of tocopherol (3). A rapid correction of the abnormal -*), high peroxide hemolysis has also been observed in vitamin r lipoprotein E-deficient premature infants given tocopherol (2). e correspondSince the addition of EDTA did not alter tocopherol in the lower cols.…”

Section: Resultsmentioning

confidence: 69%

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Tocopherol transport in the rat erythrocyte

Silber¹,

Winter²,

Kayden³

1969

J. Clin. Invest.

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A B S T R A C T The transport of vitamin E (a-tocopherol) has been studied in the rat erythrocyte in vivo and in vitro. Uptake and efflux are independent of energy, but sensitive to temperature. Tocopherol is localized to the cell membrane. Rapid exchange takes place between erythrocytes and serum with an hourly fractional tocopherol efflux of 26%. The vitamin is transferred from the erythrocyte to the low density lipoproteins. These experiments indicate that tocopherol, like cholesterol, is a constituent of the erythrocyte membrane which is in dynamic equilibrium with the corresponding plasma compound.

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Section: Resultsmentioning

confidence: 69%

“…Decreased serum levels of vitamin E (a-tocopherol) have been described in premature infants (2) and in hereditary abetalipoproteinemia (3), two conditions associated with a shortened erythrocyte survival. A favorable response to this vitamin has been reported in the anemia of prematurity (4,5).…”

Section: Introductionmentioning

confidence: 99%

Tocopherol transport in the rat erythrocyte

Silber¹,

Winter²,

Kayden³

1969

J. Clin. Invest.

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“…Previous studies have identified abnormal erythrocyte hemolysis in vitro in many, but not all, of the CF patients examined with low plasma tocopherol concentrations (1,22). In addition, earlier reports have noted that peroxide hemolysis test results are difficult to reproduce and that samples from healthy subjects with normal tocopherol levels may show as much as 10-20% hemolysis (1,14,22). Because of the difficulties encountered with other hemolysis test procedures, we devoted considerable effort in this study to establishing conditions in which: (a) reproducible results could be obtained with tocopherol-deficient erythrocytes, and (b) control preparations would not hemolyze.…”

Section: Discussionmentioning

confidence: 99%

The Occurrence and Effects of Human Vitamin E Deficiency

Farrell¹,

Bieri²,

Fratantoni³

et al. 1977

J. Clin. Invest.

237

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A B S T R A C T The role of vitamin E in human nutrition was studied by investigation of patients with cystic fibrosis (CF) and associated pancreatic insufficiency. Vitamin E status was assessed by measurement of the plasma concentration of the principal circulating isomer, a-tocopherol. Results of such determinations in 52 CF patients with pancreatogenic steatorrhea revealed that all were deficient in the vitamin. The extent of decreased plasma tocopherol varied markedly but correlated with indices of intestinal malabsorption, such as the serum carotene concentration and percentage of dietary fat absorbed. Supplementation with 5-10 times the recommended daily allowance of vitamin E in a water-miscible form increased the plasma a-tocopherol concentrations to normal in all 19 CF patients so evaluated.Studies on the effects of vitamin E deficiency focused on possible hematologic alterations. An improved technique was developed to measure erythrocyte hemolysis in vitro in the presence of hydrogen peroxide. While erythrocyte suspensions from control subjects demonstrated resistance to hemolysis during a 3-h incubation, all samples from tocopherol-deficient CF patients showed abnormal oxidant susceptibility, evidenced by greater than 5% hemoglobin release. The degree of peroxide-induced hemolysis was related to the plasma a-tocopherol concentration in an inverse, Dr. Farrell's present address and that for reprint requests is

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“…The susceptibility of neonatal erythrocytes to hemolysis in dilute hydrogen peroxide solution has been known for many years [14]. Susceptibility of a cell to peroxide hemolysis indicates the rate at which erythrocyte fatty acids can be oxidized and is dependent on a complex balance of prooxidants and antioxidants [16].…”

Section: Discussionmentioning

confidence: 99%

Erythrocyte Lipids in the Neonate

Neerhout

1968

Pediatr Res

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The lipid composition has been characterized in erythrocytes obtained from the cord blood of fullterm normal infants. There is an increase in total lipid, lipid phosphorous and cholesterol per cell (total lipid = 6.45 X 10" 10 mg; Lipid P = 1.54 X 10" 11 mg; cholesterol = 1.79 X 10" 10 mg) when compared with adult controls (total lipid = 5.07 X 10" 10 mg; Lipid P = 1.22 X 10" 11 mg; cholesterol = 1.33 X 10~1 0 mg). Despite the increased lipid content, the percentages of total lipid comprised by lipid phosphorous and cholesterol are similar to those found in the adult (P = 2.40 % of total lipid in infants, 2.41 % in adults: cholesterol = 27.1 % of total lipid in infants, 26.0 % in adults). Phospholipid fractionation shows minor variations between the two groups. Cord blood erythrocyte phospholipid has 1.0% lysolecithin, 26.0% sphingomyelin, 27.7% phosphatidylcholine, 15.2% combined phosphatidylserine and phosphatidylinositol, and 29.1 % phosphatidylethanolamine. Adult erythrocyte phospholipid has 1.2% lysolecithin, 24.1% sphingomyelin, 29.5% phosphatidylcholine, 13.1% combined phosphatidylserine and phosphatidylinositol and 31.2% phosphatidylethanolamine. Phospholipid fatty acid patterns in cord blood erythrocytes show an increased percentage of palmitic acid (cord = 21.3%, adult = 17.0%), stearic acid (cord = 16.3%, adult = 15.3%) arachidonic acid (cord = 19.6%, adult = 17.4%) and combined 22 and 24 carbon fatty acids (cord = 17.6%, adult = 16.3%) associated with decreased percentages of oleic acid (cord = 11.9%, adult = 14.6%) and linoleic acid (cord = 3.4%, adult = 10.9%). SpeculationThe erythrocyte lipids of the newborn show deviations from the adult pattern which may have adaptive value for intrauterine life. These same adaptations may render the cell more vulnerable to oxidative damage in postnatal life. Introductionproportion of phosphatidylcholine and phosphatidylethanolamine and the presence of phosphatidylserine The lipid composition of erythrocytes has recently be-imprint a pattern of erythrocyte lipid in the adult come a matter of great interest. Studies have shown clearly different from that of the plasma-environment, that the red cell lipid is composed almost entirely of Adult plasma has two-thirds of the cholesterol in the free cholesterol and phospholipid [32,40]. The minute esterified form, a proportion of phosphatidylcholine amounts of cholesterol esters, the roughly equivalent ten times that of phosphatidylethanolamine and lacks

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Studies of Tocopherol Deficiency in Infants and Children<subtitle>I. Hemolysis of Erythrocytes in Hydrogen Peroxide</subtitle>

Cited by 55 publications

References 27 publications

Tocopherol transport in the rat erythrocyte

Tocopherol transport in the rat erythrocyte

The Occurrence and Effects of Human Vitamin E Deficiency

Erythrocyte Lipids in the Neonate

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