Studies of the morphology of Murray Valley encephalitis and Japanese encephalitis viruses growing in cultured mosquito cells

Electron microscopic observations onAedes albopictus cells infected with dengue viruses

“…Most of these observations are consistent in the points that dengue virion morphogenesis takes place in close relationship with the intracellular membranes, especially R E R membranes, and that mature virions are observed mostly in the eisternae of these membranous structures. Such fundamental characteristics of virion morphogenesis appear to be common features in ftavivirus infected vertrebrate cells (1,2,3,4,5,6,7,10,11,18,23,26,29,31,34,35,36,39,41,47,49) or mosquito cells (14,21,48). Our observations in dengue infected A. albopictus cells are also consistent with these points.…”

Zika virus infection of the central nervous system of mice

“…Murray Valley encephalitis (MVE) virus grown in mosquito cell cultures produces virus factories similar to those described here (14). JBE virus however, replicates in the endoplasmie reticulum of these cells without the formation of a definite inclusion body (14), while in porcine kidney cells JBE virus appears to form by budding from the surface of cytoplasmic vacuoles (15). Finally, Wesselsbron virus produces crystalline and "honey-comb" pattern inclusion bodies in foetal lamb kidney cells with mature particles present in endoplasmic eisternae (16).…”

Replication of Flaviviruses

“…The lamellae of smooth membranes in Kunjin-infected cells have been found to open directly to the exterior (Westaway, 1980). Viruscontaining vesicles have been observed to move to the periphery of the cytoplasm and fuse with the plasma membrane (Filshie and Rehacek, 1968;Dalton, 1972). The final processing of the M protein apparently occurs during the release of virions from cells.…”