Studies of the immunbiologic importance of soluble hepatic proteins

Büschenfelde, K H Meyer zum; Kößling, F. K.

doi:10.1007/bf02044245

Cited by 18 publications

(1 citation statement)

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“…Most investigators, however, gave much attention to particulate liver fractions and to a family of remarkably thermostable antigens referred to as BE antigens for the resistance to boiling and ethanol precipita tion [2,13]. Relatively little work has been done with antigens prepared from the solu ble liver protein fraction [3,10]. Very re cently the isolation and characterization of a liver-specific macrolipoprotein was reported [11].…”

Section: Discussionmentioning

confidence: 99%

Preparation and Properties of a Specific Antiserum Against Human Liver

Mihas¹,

Spenney²

1977

Int Arch Allergy Immunol

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Immunization of rabbits with a soluble human liver fraction incorporated into Freund’s adjuvants produced antibodies that reacted with human liver extracts as well as extracts of other human organs. Absorption of the antihuman liver sera (AHLS) with lyophilized human serum, heart, and kidney abolished the reactions with other organs but did not remove the liver antibodies. The existence of at least two different liver antigens could be shown by gel diffusion precipitation experiments. One of these antigens could be removed by absorption of the antisera with chicken liver extracts. The AHLS did not cross-react with liver extracts of other species. Comparison of the AHLS with a mouse liver anti-serum described previously indicated that these antisera detected closely related but non-identical liver antigens.

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Section: Discussionmentioning

confidence: 99%