Studies of the humoral factors produced by layered chondrocyte sheets

Hamahashi, Kosuke; Sato, Masato; Yamato, Masayuki; Kokubo, Mami; Mitani, Genya; Ito, Satoshi; Nagai, Toshihiro; Ebihara, Goro; Kutsuna, Toshiharu; Okano, Teruo; Mochida, Joji

doi:10.1002/term.1610

Cited by 36 publications

(47 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hamahashi et al reported that a layered chondrocyte sheet produced the most humoral factors, including PGE2, which plays a key role in its anti-inflammatory function. 153 In chondrocyte sheets, catabolic factors such as MMP3, MMP13, and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) were also observed to decrease, while the expression of TIMP1 with antagonistic actions against MMP3 increased.…”

Section: 151mentioning

confidence: 99%

Anti-Inflammatory Strategies in Cartilage Repair

Zhang

Pizzute

Pei

2014

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

Section: 151mentioning

confidence: 99%

Anti-Inflammatory Strategies in Cartilage Repair

Zhang

Pizzute

Pei

2014

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

“…21 In addition, Hamahashi et al showed that layered chondrocyte sheets produced higher levels of TGFb and PGE2 as humoral factors compared with monolayer chondrocyte sheets and suspected that these factors might contribute to cartilage repair. 22 However, these previously reported results might not…”

Section: Discussionmentioning

confidence: 79%

Repair Mechanism of Osteochondral Defect Promoted by Bioengineered Chondrocyte Sheet

Shimizu

Kamei

Adachi

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

Cell sheet engineering has developed as a remarkable method for cell transplantation. In the field of cartilage regeneration, several studies previously reported that cartilage defects could be regenerated by transplantation of a chondrocyte sheet using cell sheet engineering. However, it remains unclear how such a thin cell sheet could repair a deep cartilage defect. We, therefore, focused on the mechanism of cartilage repair using cell sheet engineering in this study. Chondrocyte sheets and synovial cell sheets were fabricated using cell sheet engineering, and these allogenic cell sheets were transplanted to cover an osteochondral defect in a rat model. Macroscopic and histological evaluation was performed at 4 and 12 weeks after transplantation. Analysis of the gene expression of each cell sheet and of the regenerated tissue at 1 week after transplantation was performed. In addition, green fluorescent protein (GFP) transgenic rats were used as donors (transplanted chondrocyte sheets) or recipients (osteochondral defect models) to identify the cell origin of regenerated cartilage.Cartilage repair was significantly better in the group implanted with a chondrocyte sheet than in that with a synovial cell sheet. The results of gene expression analysis suggest that the possible factor contributing to cartilage repair might be TGFb1. Cell tracking experiments using GFP transgenic rats showed that the regenerated cartilage was largely composed of cells derived from the transplanted chondrocyte sheets.

show abstract

“…We found that the formation of a chondrocyte sheet structure enhanced transforming growth factor-β secretion from the cells [37], which implies that maintaining the membranous structure after cryopreservation is a prerequisite for function. Therefore, in the present study, we focused on both the maintenance of sheet structures and of cell viability after vitrification.…”

Section: Resultsmentioning

confidence: 99%

Development of a novel vitrification method for chondrocyte sheets

et al. 2013

View full text Add to dashboard Cite

BackgroundThere is considerable interest in using cell sheets for the treatment of various lesions as part of regenerative medicine therapy. Cell sheets can be prepared in temperature-responsive culture dishes and applied to injured tissue. For example, cartilage-derived cell sheets are currently under preclinical testing for use in treatment of knee cartilage injuries. The additional use of cryopreservation technology could increase the range and practicality of cell sheet therapies. To date, however, cryopreservation of cell sheets has proved impractical.ResultsHere we have developed a novel and effective method for cryopreserving fragile chondrocyte sheets. We modified the vitrification method previously developed for cryopreservation of mammalian embryos to vitrify a cell sheet through use of a minimum volume of vitrification solution containing 20% dimethyl sulfoxide, 20% ethylene glycol, 0.5 M sucrose, and 10% carboxylated poly-L-lysine. The principal feature of our method is the coating of the cell sheet with a viscous vitrification solution containing permeable and non-permeable cryoprotectants prior to vitrification in liquid nitrogen vapor. This method prevented fracturing of the fragile cell sheet even after vitrification and rewarming. Both the macro- and microstructures of the vitrified cell sheets were maintained without damage or loss of major components. Cell survival in the vitrified sheets was comparable to that in non-vitrified samples.ConclusionsWe have shown here that it is feasible to vitrify chondrocyte cell sheets and that these sheets retain their normal characteristics upon thawing. The availability of a practical cryopreservation method should make a significant contribution to the effectiveness and range of applications of cell sheet therapy.

show abstract

Studies of the humoral factors produced by layered chondrocyte sheets

Cited by 36 publications

References 36 publications

Anti-Inflammatory Strategies in Cartilage Repair

Anti-Inflammatory Strategies in Cartilage Repair

Repair Mechanism of Osteochondral Defect Promoted by Bioengineered Chondrocyte Sheet

Development of a novel vitrification method for chondrocyte sheets

Contact Info

Product

Resources

About