Anti-Inflammatory Strategies in Cartilage Repair

Zhang, Ying; Pizzute, Tyler; Pei, Ming

doi:10.1089/ten.teb.2014.0014

Cited by 52 publications

(38 citation statements)

References 150 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, PL has been reported to have an anti-inflammatory and analgesic effect in some tissues, including cartilage. It may enhance the healing of injured cartilage, which it is easily attacked by proinflammatory factors and affected by oxidative stress [23, 24]. …”

Section: Discussionmentioning

confidence: 99%

Platelet lysate induces chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells

et al. 2018

View full text Add to dashboard Cite

PurposeArticular cartilage has a poor capacity for self-repair, and thus still presents a major challenge in orthopedics. Mesenchymal stem cells (MSCs) are multipotent stem cells with the potential to differentiate into chondrocytes in the presence of transforming growth factor beta (TGF-β). Platelet lysate (PL) contains a relatively large number of growth factors, including TGF-β, and has been shown to ameliorate cartilage repair. Here, we investigated the ability of PL to direct chondrogenic differentiation of MSCs along with other standard differentiation components in a pellet culture system.MethodsWe isolated and expanded MSCs from human umbilical cords using a PL-supplemented medium and characterized the cells based on immunophenotype and potential for differentiation to adipocytes and osteocytes. We further cultured MSCs as pellets in a chondrogenic-differentiation medium supplemented with PL. After 21 days, the pellets were processed for histological analysis and stained with alician blue and acridine orange. The expression of SOX9 was investigated using RT-PCR.ResultsMSCs maintained their stemness characteristics in the PL-supplemented medium. However, the distribution of cells in the pellets cultured in the PL-supplemented chondrogenic differentiation medium had a greater similarity to cartilage tissue-derived chondrocytes than to the negative control. The intense alician blue staining indicated an increased production of mucopolysaccharides in the differentiated pellets, which also showed elevated expression of SOX9.ConclusionsOur data suggest that MSCs could be differentiated to chondrocytes in the presence of PL and absence of exogenous TGF-β. Further research needs to be conducted to understand the exact role and potential of PL in chondrogenic differentiation and chondrocyte regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Platelet lysate induces chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Cartilage tissue engineering has emerged as a potential therapeutic option for cartilage repair; however, once transplanted into the joints, cells or premature cartilage often face an inflammatory environment, making cartilage resurfacing challenging [13]. Among the proinflammatory cytokines, IL-1β acts as an important mediator of increased ECM degradation by stimulating a number of events, such as an increase of MMPs and nitric oxide production [23] and by suppressing type II collagen and proteoglycan synthesis [24].…”

Section: Discussionmentioning

confidence: 99%

“…Different species and donor age might cause this discrepancy [7]. Furthermore, posttraumatic joint inflammation, normally accompanying cartilage defects [11,12], could perhaps lead to reduced efficiency of dECM on the rejuvenation of adult hSDSCs [13]. …”

Section: Introductionmentioning

confidence: 99%

sb203580 preconditioning recharges matrix-expanded human adult stem cells for chondrogenesis in an inflammatory environment – A feasible approach for autologous stem cell based osteoarthritic cartilage repair

Zhang

Pizzute

et al. 2015

Biomaterials

Self Cite

View full text Add to dashboard Cite

Autologous stem cells are a promising cell source for cartilage regeneration; however, cell replicative senescence and joint posttraumatic inflammation provide challenges to bring this treatment modality to fruition. In this study, we hypothesized that preconditioning with p38 MAPK inhibitor (sb203580) would recharge decellularized extracellular matrix (dECM) expanded human synovium-derived stem cell (hSDSC) chondrogenesis in an inflammatory environment. We found that preconditioning with sb203580 greatly enhanced dECM expanded hSDSC proliferation and chondrogenic potential while supplementation with sb203580 in an induction medium dramatically retarded hSDSC chondrogenic differentiation, even for dECM expanded cells. We also found that sb203580 preconditioning enhanced matrix-expanded hSDSC chondrogenic capacity even in an interleukin-1 (IL-1) induced inflammatory environment. Non-detectable expression of HLA-DR in the hSDSCs grown on allogeneic dECM indicates the feasibility of commercial preparation of these dECMs from healthy, young donors for patients who need autologous transplantation. Our study indicated that p38 MAPK inhibitor has a distinctive priming effect on dECM mediated stem cell cartilage regeneration. Combined rejuvenation with sb203580 and dECM expansion can precondition hSDSCs’ resurfacing capacity for osteoarthritic patients with cartilage defects.

show abstract

“…Cartilage defects usually accompanied with posttraumatic inflammation present a challenge in cartilage repair and the biological constructs for implantation need to be able to survive this harsh environment [128]. Pei et al found that expansion on dECM promoted the antioxidative and chondrogenic capacities of human adult SDSCs, slowing down the decrease of cell proliferation and the increase in apoptosis, and contributed SDSCs’ resistance to cell-cycle G1 arrest resulting from hydrogen peroxide [116].…”

Section: Decellularized Ecm (Decm) Preconditioningmentioning

confidence: 99%

Environmental preconditioning rejuvenates adult stem cells' proliferation and chondrogenic potential

Pei

2017

Biomaterials

Self Cite

View full text Add to dashboard Cite

Adult stem cells are a promising cell source for cartilage regeneration. Unfortunately, due to donor age and ex vivo expansion, stem cell senescence becomes a huge hurdle for these cells to be used clinically. Increasing evidence indicates that environmental preconditioning is a powerful approach in promoting stem cells’ ability to resist a harsh environment post-engraftment, such as hypoxia and inflammation. However, few reports organize and evaluate the literature regarding the rejuvenation effect of environmental preconditioning on stem cell proliferation and chondrogenic differentiation capacity, which are important variables for stem cell based tissue regeneration. This report aims to identify several critical environmental factors such as oxygen concentration, growth factors, and extracellular matrix and to discuss their preconditioning influence on stem cells’ rejuvenation including proliferation and chondrogenic potential as well as underlying molecular mechanisms. We believe that environmental preconditioning based rejuvenation is a simpler and safer strategy to program pre-engraftment stem cells for better survival and enhanced proliferation and differentiation capacity without the undesired effects of some treatments, such as genetic manipulation.

show abstract

Anti-Inflammatory Strategies in Cartilage Repair

Cited by 52 publications

References 150 publications

Platelet lysate induces chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells

Platelet lysate induces chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells

sb203580 preconditioning recharges matrix-expanded human adult stem cells for chondrogenesis in an inflammatory environment – A feasible approach for autologous stem cell based osteoarthritic cartilage repair

Environmental preconditioning rejuvenates adult stem cells' proliferation and chondrogenic potential

Contact Info

Product

Resources

About